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Wyświetlanie 1-10 z 58
Tytuł:
Determination of plasma bone-specific alkaline phosphatase isoenzyme activity in Holstein calves using a commercial agarose gel electrophoresis kit
Autorzy:
Onomi, R.
Hatate, K.
Yamagishi, N.
Tematy:
agarose gel electrophoresis (AGE)
bone-specific alkaline phosphatase isoenzyme
calf
Pokaż więcej
Data publikacji:
2019
Powiązania:
https://bibliotekanauki.pl/articles/2087395.pdf  Link otwiera się w nowym oknie
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 789-792
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isoenzyme markers of two hepatic species: Barbilophozia lycopodioides [Wallr.] Loeske, and B. hatcheri [A. Evans] Loeske
Autorzy:
Baczkiewicz, A
Buczkowska, K.
Lembicz, M.
Tematy:
Tatras Mountains
Polska
Barbilophozia hatcheri
plant community
Barbilophozia lycopodioides
isoenzyme marker
population
hepatic species
Pokaż więcej
Data publikacji:
2003
Powiązania:
https://bibliotekanauki.pl/articles/57952.pdf  Link otwiera się w nowym oknie
Źródło:
Acta Societatis Botanicorum Poloniae; 2003, 72, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Opis:
Two closely related species of the genus Barbilophozia: B. lycopodioides and B. hatcheri were studied in populations from the Tatra Range (S Poland), where they are frequent and widely distributed. Both species play an important role in plant communities and grow here very often side by side. Typically developed plants are quite easy to distinguish (even in the field), however morphologically intermediate forms, difficult to recognize by using of classical taxonomic methods, sometimes are found. We found enzymatic markers, that allow to recognize the critical forms. Both studied species are different in enzymatic patterns of glutamate oxaloacetate transaminase (GOT) and peroxidases (PX). In GOT four different phenotypes were detected. The first two (GOT 1 and GOT 2) were characteristic for B. hatcheri and next two (GOT 3 and GOT 4) for B. lycopodioides. Peroxidase patterns, that were monomorphic and specific for each species, exhibit different mobility in anodal and cathodal parts of gel. Results of the studies allowed us to draw the conclusion, that PX and GOT are good isoenzymatic markers and they can have practical application for identification of Barbilophozia species.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induced isozyme polymorphism in spring barley mutants
Autorzy:
Kucharska, M
Maluszynski, M
Tematy:
polymorphism
isoenzyme
barley
induced mutant
mutant
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Data publikacji:
1996
Powiązania:
https://bibliotekanauki.pl/articles/2047711.pdf  Link otwiera się w nowym oknie
Źródło:
Journal of Applied Genetics; 1996, 37, 1; 1-9
1234-1983
Pojawia się w:
Journal of Applied Genetics
Opis:
The usefulness of mutagenic treatment to enlarge isozymic variability of barley and the use of induced mutants for genetic analysis were evaluated. N-methyl-N-nitroso urea, sodium azide and gamma rays were employed as mutagenic agents. Electrophoretic assays of 3848 M₂ seedlings obtained by chemical mutagenic treatment of the spring barley cultivars Dema, Aramir, Bielik and 3100 M₂ seedlings obtained by physical mutagenic treatment of the cv. Dema revealed 70 isozymic mutants, which represent 30 separate mutants in 25 M₁ plants. Most of mutations (27) were induced by chemical mutagen at polymorphic esterase loci. The occurrence of induced mutants at monomorphic loci, Got2 and Lap2, made it possible to perform genetic analysis of those loci in barley including mapping respective genes within chromosomes.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Role of oligochitosans in regulation of cellular activity and location of pyruvate kinase (pk) isoenzyme m2 that affects proliferation of ehrlich ascites tumor cells (eat)
Autorzy:
Ignacak, Jan
Wiśniewska-Wrona, Maria
Pałka, Iwona
Niekraszewicz, Antoni
Tematy:
isoenzyme M1 and M2 pyruvate kinase
nucleus
oligochitosans
ε-methyl-L-lysyne
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Data publikacji:
2013
Powiązania:
https://bibliotekanauki.pl/articles/1035231.pdf  Link otwiera się w nowym oknie
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2013, 18, 18; 67-76
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Opis:
The pyruvate kinase isoenzyme M2 originating from the nucleoplasm and cytoplasm of tumor cells, with its highest affinity to the 2-phosphoenolpyruvate (2-PEP) and sensitivity to L-cysteine, contributes to an increased generation of energy as ATP, necessary for tumor cell proliferation. In the presence of L-cysteine, the isoenzyme M2 PK demonstrates the activity of histone kinase, transferring the phosphoryl group from 2-PEP to the ε-amine residue of the H1 histone lysine. Oligochitosans induce expression of the inducible nitric oxide synthase gene (iNOS), what results in an increased synthesis of nitric oxide, which reacts with L-cysteine and produces L-S-nitrosocysteine. Lack of L-cysteine contributes to inhibition of kinase activity of the H1 histone, an M2 PK isoenzyme. Decreased phosphorylation of the H1 histone contributes to inhibition of EAT cell proliferation. No effect on proliferation of normal cells that include the PK M1 isoenzyme has been observed in the presence of oligochitosans.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Arginase isoenzymes in human cirrhotic liver
Autorzy:
Chrzanowska, Alicja
Gajewska, Beata
Barańczyk-Kuźma, Anna
Tematy:
arginase activity
liver cirrhosis
isoenzyme expression
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Data publikacji:
2009
Powiązania:
https://bibliotekanauki.pl/articles/1040542.pdf  Link otwiera się w nowym oknie
Źródło:
Acta Biochimica Polonica; 2009, 56, 3; 465-469
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Opis:
Cirrhosis leads to an inability of the liver to perform its biochemical functions. It can also lead to hepatocellular carcinoma in which, as we showed lately, arginase isoenzyme pattern changes. The present work presents our results on arginase isoenzymes and their possible role in liver cirrhosis. The study was performed on tissues obtained during liver transplantation from 60 patients with liver cirrhosis, and on samples of histologically normal liver (control) from 40 patients with benign or colorectal cancer liver metastases removed during surgery, 6-7 cm from the tumor border. Arginase isoenzymes AI (so-called liver-type arginase) and AII (called extrahepatic arginase) were identified by Western blotting and isolated by ion-exchange chromatography. Their expression on mRNA level was studied by RT-PCR. A significant decrease in arginase activity, dependent of the liver clinical stage, was observed in cirrhotic tissue. Arginase AI activity and its mRNA level were significantly decreased in cirrhotic liver, whereas the activity and expression of arginase AII were concurrently raised, as compared to normal liver. Since arginase AI is a key enzyme of the urea cycle, whereas arginase AII most probably takes part in the biosynthesis of ornithine and polyamines, the defective ammonia inactivation and increased collagen biosynthesis observed in cirrhotic liver may be related to the changes in arginase AI and AII levels, respectively.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Role of oligochitosans in regulation of cellular activity and location of pyruvate kinase (pk) isoenzyme m2 that affects proliferation of ehrlich ascites tumor cells (eat)
Autorzy:
Ignacak, Jan
Wiśniewska-Wrona, Maria
Pałka, Iwona
Niekraszewicz, Antoni
Tematy:
isoenzyme M1 and M2 pyruvate kinase
nucleus
oligochitosans
ε-methyl-L-lysyne
Pokaż więcej
Data publikacji:
2013
Powiązania:
https://bibliotekanauki.pl/articles/1035209.pdf  Link otwiera się w nowym oknie
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2013, 18, 18; 67-76
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Opis:
The pyruvate kinase isoenzyme M2 originating from the nucleoplasm and cytoplasm of tumor cells, with its highest affinity to the 2-phosphoenolpyruvate (2-PEP) and sensitivity to L-cysteine, contributes to an increased generation of energy as ATP, necessary for tumor cell proliferation. In the presence of L-cysteine, the isoenzyme M2 PK demonstrates the activity of histone kinase, transferring the phosphoryl group from 2-PEP to the ε-amine residue of the H1 histone lysine. Oligochitosans induce expression of the inducible nitric oxide synthase gene (iNOS), what results in an increased synthesis of nitric oxide, which reacts with L-cysteine and produces L-S-nitrosocysteine. Lack of L-cysteine contributes to inhibition of kinase activity of the H1 histone, an M2 PK isoenzyme. Decreased phosphorylation of the H1 histone contributes to inhibition of EAT cell proliferation. No effect on proliferation of normal cells that include the PK M1 isoenzyme has been observed in the presence of oligochitosans.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proposal for a seed certification scheme
Autorzy:
Behm, A.
Konnert, M.
Tematy:
representative sample
seed quality
certification scheme
isoenzyme
plant quality
seed
Pokaż więcej
Data publikacji:
2002
Powiązania:
https://bibliotekanauki.pl/articles/41261.pdf  Link otwiera się w nowym oknie
Źródło:
Dendrobiology; 2002, 47 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Opis:
Identity of reproductive material is essential in artificial forest regeneration. Legal regulations cannot guarantee proof of identity. State and private organizations have been cooperating to develop a seed certification scheme in forestry. Representative samples of forest reproductive material are taken at any production stage starting at the time of seed collection through to plant delivery as well as at time of changing ownership.The first sample at the time of seed harvest is used to determine the maximum potential yield of living germinants from the total collection. Furhtermore this and the other samples are tested randomly at a determined level of intensity or in case of doubt by Isozyme/DNA methods. Sampling techniques have been tested and described in a handbook. The biochemical-genetic tests are being standardized for all major commercial species. Forest reproductive material having been sampled in the described way will be certified "of provable identity" by a neutral agency. The Scheme will be open to anyone complying with the rules laid down. Larger forest owners have confirmed to preferably purchase "forest plants of provable identity".
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Class III peroxidases in maize plasma membranes
Autorzy:
Moller, B.
Luthje, S.
Tematy:
conference
class III peroxidase
maize
plasma membrane
isoenzyme
thylakoid membrane
tonoplast membrane
oxidative stress
Pokaż więcej
Data publikacji:
2013
Powiązania:
https://bibliotekanauki.pl/articles/79851.pdf  Link otwiera się w nowym oknie
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 58

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