Temat: DNA - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: Rapid DNA – technologia umożliwiająca zautomatyzowaną, szybką analizę profilu DNA wykorzystującą polimorfizm loci STR
Rapid DNA – a technology for rapid automated DNA profile analysis based on STR loci polymorphism
Autorzy:: Kartasińska, Ewa
Jurga, Anna
Tematy:: rapid DNA
profil DNA
STR
CODIS
baza danych DNA
DNA profile
DNA database; Pokaż więcej
Data publikacji:: 2020
Powiązania:: https://bibliotekanauki.pl/articles/2057461.pdf Link otwiera się w nowym oknie
Źródło:: Problemy Kryminalistyki; 2020, 309; 5-12
0552-2153
Pojawia się w:: Problemy Kryminalistyki
Opis:: Od połowy lat dziewięćdziesiątych ubiegłego wieku badania DNA stały się synonimem postępu naukowego w technice kryminalistycznej. Profilowanie wykorzystujące analizę polimorfizmu loci STR uznawane jest za tzw. złoty standard i stanowi nieocenione źródło informacji umożliwiające m.in. identyfikację osób podejrzanych i poszukiwanych, ustalenie tożsamości nieznanych zwłok, w tym także na podstawie analizy pokrewieństwa. Badania takie są jednak procesem praco- i czasochłonnym. Aby zredukować tę niedogodność, opracowano technologię opisywaną mianem technologii rapid DNA.
Since the mid-1990s of the last century, DNA research has become synonymous with scientific progress in forensics. DNA profiling based on the analysis of STR loci polymorphism is considered the gold standard and constitutes an invaluable source of information, enabling, inter alia, the identification of suspects and wanted persons and the identification of corpses, including that based on kinship analysis. However, such analysis is laborious and time-consuming. To reduce this inconvenience, a technology described as rapid DNA has been developed.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Profilowanie genetyczne śladów daktyloskopijnych - problem wtórengo transferu materiału
Genetic profiling from fingerprints - the issue of secondary DNA transfer
Autorzy:: Pająk, Katarzyna
Wierzchosławski, Rafał
Tematy:: śladowe ilości DNA
wtórny transfer DNA
ślady daktyloskopijne
profilowanie DNA
trace DNA
secondary DNA transfer
latent fingerprints
DNA profiling; Pokaż więcej
Data publikacji:: 2013
Powiązania:: https://bibliotekanauki.pl/articles/499824.pdf Link otwiera się w nowym oknie
Źródło:: Problemy Kryminalistyki; 2013, 280; 58-64
0552-2153
Pojawia się w:: Problemy Kryminalistyki
Opis:: Rozwój technik molekularnych umożliwił pozyskiwanie profili genetycznych ze śladowych ilości materiału biologicznego. Od połowy lat 90. coraz częściej podejmowane są próby namnażania DNA z odcisków palców. Dotychczasowe badania dowiodły, że wykorzystanie technik ujawniania śladów daktyloskopijnych nie wyklucza późniejszej analizy genetycznej. Coraz liczniejsze są jednak dowody na to, iż narzędzia stosowane do ujawniana śladów mogą akumulować materiał genetyczny i prowadzić do wtórnego transferu DNA, skutkującego zanieczyszczeniem materiału dowodowego. Konieczne wydaje się więc wprowadzenie procedur pozwalających zminimalizować ryzyko zanieczyszczenia śladu, tak aby metody identyfikacji daktyloskopijnej nie kolidowały z metodami identyfikacji genetycznej.
The improvement of molecular biology techniques allowed the obtaining of genetic profiles from minute amounts of biological material. Since the mid-90s DNA typing of latent fingerprints became a common procedure in forensic casework. Numerous studies have shown that the fingerprint detection techniques do not preclude further genetic identification. However, there is a growing evidence that the tools used in the dactyloscopic visualization can accumulate genetic material and become a source of secondary DNA transfer, resulting in contamination of traces. Therefore, it seems necessary to develop procedures that minimize the risk of evidence contamination, so that the dactyloscopic methods do not interfere with genetic identification.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: DNA-Based Criminal Investigation: Problems and Prospects
Autorzy:: Albrecht, Hans-Joerg
Tematy:: dochodzenia kryminalne
DNA
badania DNA
bazy danych DNA
DNA-based criminal investigation
criminal investigation
DNA databases; Pokaż więcej
Data publikacji:: 2008
Powiązania:: https://bibliotekanauki.pl/articles/698803.pdf Link otwiera się w nowym oknie
Źródło:: Archiwum Kryminologii; 2008, XXIX-XXX; 779-790
0066-6890
2719-4280
Pojawia się w:: Archiwum Kryminologii
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Recognition and repair of DNA-cisplatin adducts.
Autorzy:: Woźniak, Katarzyna
Błasiak, Janusz
Tematy:: DNA-protein crosslinks
cisplatin
DNA adducts
DNA damage
DNA repair
cis-diamminedichloroplatinum; Pokaż więcej
Data publikacji:: 2002
Powiązania:: https://bibliotekanauki.pl/articles/1043720.pdf Link otwiera się w nowym oknie
Źródło:: Acta Biochimica Polonica; 2002, 49, 3; 583-596
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Opis:: Anticancer activity of cisplatin (cis-diamminedichloroplatinum) is believed to result from its interaction with DNA. The drug reacts with nucleophilic sites in DNA forming monoadducts as well as intra- and interstrand crosslinks. DNA-cisplatin adducts are specifically recognized by several proteins. They can be divided into two classes. One constitutes proteins which recognize DNA damage as an initial step of the nucleotide excision and mismatch repair pathways. The other class contains proteins stabilizing cellular DNA-protein and protein-protein complexes, including non-histone proteins from the HMG (high-mobility-group) family. They specifically recognize 1,2-interstrand d(GpG) and d(ApG) crosslinks of DNA-cisplatin adducts and inhibit their repair. Many HMG-domain proteins can function as transcription factors, e.g. UBF, an RNA polymerase I transcription factor, the mammalian testis-determining factor SRY and the human mitochondrial transcription factor mtTFA. Moreover, it seems that some proteins, which probably recognize DNA-cisplatin adducts non-specifically, e.g. actin and other nuclear matrix proteins, can disturb the structural and functional organization of the nucleus and whole cell. The formation of complexes between DNA and proteins in the presence of cisplatin and the changes in the cell architecture may account for the drug cytotoxicity.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons
Autorzy:: Jeng, Hueiwang Anna
Pan, Chih-Hong
Chao, Mu-Rong
Chiu, Chien-Chih
Zhou, Guodong
Chou, Chon-Kit
Lin, Wen-Yi
Tematy:: DNA damage
semen quality
sperm
DNA integrity
DNA fragmentation
bulky DNA adducts; Pokaż więcej
Data publikacji:: 2016-11-18
Powiązania:: https://bibliotekanauki.pl/articles/2168377.pdf Link otwiera się w nowym oknie
Źródło:: International Journal of Occupational Medicine and Environmental Health; 2016, 29, 6; 915-926
1232-1087
1896-494X
Pojawia się w:: International Journal of Occupational Medicine and Environmental Health
Opis:: Objectives The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. Material and methods The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo). A questionnaire was conducted to collect demographic and potential confounding data. Results The coke oven workers had lower percentages of sperm motility, vitality and normal morphology than the control group, but the difference was not significant. For DNA integrity, the coke oven workers had significantly higher concentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048, respectively). However, DNA fragmentation percentages did not significantly increase as compared to those in the subjects from the control group (p = 0.232). There was no correlation between sperm quality parameters and DNA integrity indicators. Conclusions Occupational exposure of the coke oven workers to PAHs was associated with decreased sperm DNA integrity. Int J Occup Med Environ Health 2016;29(6):915–926
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
Autorzy:: Kaczmarczyk, D.
Kohlmann, K.
Kersten, P.
Luczynski, M.
Tematy:: izolacja DNA
DNA mikrosatelitarne
amplifikacja PCR
DNA extraction
microsatellite DNA
PCR amplification; Pokaż więcej
Data publikacji:: 2007
Powiązania:: https://bibliotekanauki.pl/articles/363088.pdf Link otwiera się w nowym oknie
Źródło:: Environmental Biotechnology; 2007, 3, 2; 44-48
1734-4964
Pojawia się w:: Environmental Biotechnology
Opis:: American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Mutations in DNA polymerase gamma cause error prone DNA synthesis in human mitochondrial disorders
Autorzy:: Copeland, William
Ponamarev, Mikhail
Nguyen, Dinh
Kunkel, Thomas
Longley, Matthew
Tematy:: aging
DNA replication
mitochondria
DNA repair
DNA polymerase; Pokaż więcej
Data publikacji:: 2003
Powiązania:: https://bibliotekanauki.pl/articles/1043659.pdf Link otwiera się w nowym oknie
Źródło:: Acta Biochimica Polonica; 2003, 50, 1; 155-167
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Opis:: This paper summarizes recent advances in understanding the links between the cell's ability to maintain integrity of its mitochondrial genome and mitochondrial genetic diseases. Human mitochondrial DNA is replicated by the two-subunit DNA polymerase γ (pol γ). We investigated the fidelity of DNA replication by pol γ with and without exonucleolytic proofreading and its p55 accessory subunit. Pol γ has high base substitution fidelity due to efficient base selection and exonucleolytic proofreading, but low frameshift fidelity when copying homopolymeric sequences longer than four nucleotides. Progressive external ophthalmoplegia (PEO) is a rare disease characterized by the accumulation of large deletions in mitochondrial DNA. Recently, several mutations in the polymerase and exonuclease domains of the human pol γ have been shown to be associated with PEO. We are analyzing the effect of these mutations on the human pol γ enzyme. In particular, three autosomal dominant mutations alter amino acids located within polymerase motif B of pol γ. These residues are highly conserved among family A DNA polymerases, which include T7 DNA polymerase and E. coli pol I. These PEO mutations have been generated in pol γ to analyze their effects on overall polymerase function as well as the effects on the fidelity of DNA synthesis. One mutation in particular, Y955C, was found in several families throughout Europe, including one Belgian family and five unrelated Italian families. The Y955C mutant pol γ retains a wild-type catalytic rate but suffers a 45-fold decrease in apparent binding affinity for the incoming dNTP. The Y955C derivative is also much less accurate than is wild-type pol γ, with error rates for certain mismatches elevated by 10- to 100-fold. The error prone DNA synthesis observed for the Y955C pol γ is consistent with the accumulation of mtDNA mutations in patients with PEO. The effects of other pol γ mutations associated with PEO are discussed.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Mammalian DNA methyltransferases
Autorzy:: Siedlecki, Pawel
Zielenkiewicz, Piotr
Tematy:: DNA methyltransferases
DNA methylation; Pokaż więcej
Data publikacji:: 2006
Powiązania:: https://bibliotekanauki.pl/articles/1041231.pdf Link otwiera się w nowym oknie
Źródło:: Acta Biochimica Polonica; 2006, 53, 2; 245-256
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Opis:: DNA methylation is an epigenetic process affecting gene expression and chromatin organization. It can heritably silence or activate transcription of genes without any change in their nucleotide sequences, and for a long time was not recognized as an important regulatory mechanism. However, during the recent years it has been shown that improper methylation, especially hypermethylation of promoter regions, is observed in nearly all steps of tumorigenesis. Aberrant methylation is also the cause of several major pathologies including developmental disorders involving chromosome instabilities and mental retardation. A great progress has been made in our understanding of the enzymatic machinery involved in establishing and maintaining methylation patterns. This allowed for the development of new diagnostic tools and epigenetic treatment therapies. The new approaches hold a great potential; several inhibitors of DNA methyltransferases have already shown very promising therapeutic effects.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: A comparison of the in vitro genotoxicity of anticancer drugs idarubicin and mitoxantrone.
Autorzy:: Błasiak, Janusz
Gloc, Ewa
Warszawski, Mariusz
Tematy:: mitoxantrone
oxidative DNA damage
DNA damage
idarubicin
comet assay
DNA methylation
DNA repair; Pokaż więcej
Data publikacji:: 2002
Powiązania:: https://bibliotekanauki.pl/articles/1043821.pdf Link otwiera się w nowym oknie
Źródło:: Acta Biochimica Polonica; 2002, 49, 1; 145-155
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Opis:: Idarubicin is an anthracycline antibiotic used in cancer therapy. Mitoxantrone is an anthracycline analog with presumed better antineoplastic activity and lesser toxicity. Using the alkaline comet assay we showed that the drugs at 0.01-10 μM induced DNA damage in normal human lymphocytes. The effect induced by idarubicin was more pronounced than by mitoxantrone (P < 0.001). The cells treated with mitoxantrone at 1 μM were able to repair damage to their DNA within a 30-min incubation, whereas the lymphocytes exposed to idarubicin needed 180 min. Since anthracyclines are known to produce free radicals, we checked whether reactive oxygen species might be involved in the observed DNA damage. Catalase, an enzyme inactivating hydrogen peroxide, decreased the extent of DNA damage induced by idarubicin, but did not affect the extent evoked by mitoxantrone. Lymphocytes exposed to the drugs and treated with endonuclease III or formamidopyrimidine-DNA glycosylase (Fpg), enzymes recognizing and nicking oxidized bases, displayed a higher level of DNA damage than the untreated ones. 3-Methyladenine-DNA glycosylase II (AlkA), an enzyme recognizing and nicking mainly methylated bases in DNA, increased the extent of DNA damage caused by idarubicin, but not that induced by mitoxantrone. Our results indicate that the induction of secondary malignancies should be taken into account as side effects of the two drugs. Direct strand breaks, oxidation and methylation of the DNA bases can underlie the DNA-damaging effect of idarubicin, whereas mitoxantrone can induce strand breaks and modification of the bases, including oxidation. The observed in normal lymphocytes much lesser genotoxicity of mitoxantrone compared to idarubicin should be taken into account in planning chemotherapeutic strategies.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Muzealnictwo: nowoczesne technologie w służbie historii. Część pierwsza: Kopalne DNA – historia odkryć, perspektywy i problemy
Museology: modern technologies in service of history Part 1: Ancient DNA – the history of discoveries, new perspectives, and problems
Autorzy:: Pluta, Dominika
Janiszewska, Ewa
Bonar, Małgorzata
Alama, Aneta
Dobosz, Tadeusz
Tematy:: DNA
aDNA
izolacja DNA
DNA isolation; Pokaż więcej
Data publikacji:: 2018
Powiązania:: https://bibliotekanauki.pl/articles/61043066.pdf Link otwiera się w nowym oknie
Źródło:: Opuscula Musealia; 2018, 25; 93-99
0239-9989
2084-3852
Opis:: For centuries, humankind has been fascinated by the possibilities of exploring the past. People search for answers to significant questions regarding evolution, the origin of species and pathogens, and so on. Using advanced methods, museum institutions have created an opportunity to study major specimens from the past, bringing us closer to understanding these fundamental issues. Until the 1980s, studying objects from museums’ collections, concerning human, plant or animal evolutionary development, were conducted using methods based on anatomical or morphological findings. However, the results were often inaccurate and of solely theoretical value. The use of genetic methods including DNA analysis of museum objects has brought new perspectives for historians, archaeologist, anthropologists, and zoologists.
Dostawca treści:: Biblioteka Nauki

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