- Tytuł:
- An extensive quality control and quality assurance (QC/QA) program significantly improves inter-laboratory concordance rates of flow-cytometric minimal residual disease assessment in acute lymphoblastic leukemia : an I-BFM-FLOW-Network report
- Autorzy:
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Scarparo, Pamela
Groeneveld-Krentz, Stefanie
Dubravcic, Klara
Moller, Claudia
Ratei, Richard
Rodriguez, Cecilia
Kloboves Prevodnik, Veronika
Popov, Alexander
Giordano, Hugo
Svec, Peter
Palazzi, Jorge
Anastasiou-Grenzelia, Theodora
Mejstrikova, Ester
Hrusak, Ondrej
Barcala, Virna
Szczepański, Tomasz
Deniz, Gunnur
Malusardi, Cecilia
Kiss, Csongor
Buldini, Barbara
Martin, Bibiana Maria
Gaipa, Giuseppe
Batinic, Drago
Campbell, Myriam
Paterakis, Georgios
Varotto, Elena
Barna, Gabor
Biondi, Andrea
Bruggemann, Monika
Dworzak, Michael N.
Gaillard, Marie Isabel
Slavkovic, Bojana
Basso, Giuseppe
Sala, Simona
Kohlscheen, Saskia
Sartor, Mary
Sędek, Łukasz
Burnusuzov, Hasan
Vernitsky, Helly
Bourquin, Jean-Pierre
Conter, Valentino
Felice, Marisa
Nikulshin, Sergey
Schumich, Angela
Rossi, Jorge
Suzan, Adin Cinar
Gonzalez, Alejandro
Torrebadell, Montserrat
Attarbaschi, Andishe
Bukowska-Straková, Karolina
Iarossi, Maria Belen
Feuerstein, Tamar
Tzanoudaki, Marianna
Kappelmayer, Janos
Kreminska, Elena
Solari, Liliana
Carelli, Daniela
Schrappe, Martin
Jakso, Pal
Agriello, Evangelina
Maglia, Oscar
Karawajew, Leonid
Galeano, Adriana
Marinov, Neda
Samardzija, Gordana
Taparkou, Anna
Maurer-Granofszky, Margarita
Sajaroff, Elisa Olga
Hevessy, Zsuzsanna - Opis:
- Monitoring of minimal residual disease (MRD) by flow cytometry (FCM) is a powerful prognostic tool for predicting outcomes in acute lymphoblastic leukemia (ALL). To apply FCM-MRD in large, collaborative trials, dedicated laboratory staff must be educated to concordantly high levels of expertise and their performance quality should be continuously monitored. We sought to install a unique and comprehensive training and quality control (QC) program involving a large number of reference laboratories within the international Berlin-Frankfurt-Münster (I-BFM) consortium, in order to complement the standardization of the methodology with an educational component and persistent quality control measures. Our QC and quality assurance (QA) program is based on four major cornerstones: (i) a twinning maturation program, (ii) obligatory participation in external QA programs (spiked sample send around, United Kingdom National External Quality Assessment Service (UK NEQAS)), (iii) regular participation in list-mode-data (LMD) file ring trials (FCM data file send arounds), and (iv) surveys of independent data derived from trial results. We demonstrate that the training of laboratories using experienced twinning partners, along with continuous educational feedback significantly improves the performance of laboratories in detecting and quantifying MRD in pediatric ALL patients. Overall, our extensive education and quality control program improved inter-laboratory concordance rates of FCM-MRD assessments and ultimately led to a very high conformity of risk estimates in independent patient cohorts.
- Dostawca treści:
- Repozytorium Uniwersytetu Jagiellońskiego
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