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Wyświetlanie 1-6 z 6
Tytuł:
Nowe trendy w usuwaniu azotu amonowego ze ścieków: nitrytacja – anammox w niskiej temperaturze
new trends in ammonia nitrogen removal from wastewater: nitritation – anammox at low temperature
Autorzy:
Tomaszewski, M.
Cema, G.
Ziembińska-Buczyńska, A.
Tematy:
nitrytacja–anammox
usuwanie azotu amonowego
oczyszczanie ścieków
nitritation–anammox
ammonia nitrogen removal
wastewater treatment
Pokaż więcej
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Powiązania:
https://bibliotekanauki.pl/articles/400316.pdf  Link otwiera się w nowym oknie
Opis:
Proces częściowej nitryfikacji (nitrytacji) – anammox (beztlenowego utleniania azotu amonowego) znajduje coraz szersze zastosowanie w oczyszczaniu ścieków o wysokim ładunku azotu amonowego i wysokiej temperaturze (25–40°C). Podyktowane jest to optymalną temperaturą rozwoju bakterii anammox, która mieści się w zakresie od 30 do 40°C. Ze względu na korzyści płynące z wykorzystania procesu anammox do oczyszczania ścieków komunalnych, których temperatura znacznie odbiega od optymalnej dla procesu, coraz więcej uwagi poświęca się badaniom nad możliwościami efektywnego prowadzenia procesu nitrytacji – anammox w niższej temperaturze (10–20°C). W porównaniu do tradycyjnego systemu nitryfikacji – denitryfikacji, proces nitrytacji – anammox cechuje się niższym zapotrzebowaniem na tlen, niższym przyrostem osadu nadmiernego oraz brakiem zapotrzebowania na źródło węgla organicznego, co przekłada się na znaczne obniżenie kosztów eksploatacyjnych. W prezentowanej pracy dokonano przeglądu najnowszych badań i osiągnięć dotyczących zastosowania procesu nitrytacji – anammox w niskich temperaturach. Wykazały one, że możliwe jest skuteczne usuwanie azotu amonowego ze ścieków komunalnych w temperaturze 15°C w skali pilotażowej, a nawet 12°C w skali laboratoryjnej. Najlepsze rezultaty osiągane są dzięki zastosowaniu reaktorów sekwencyjnych i/lub ze złożem ruchomym, w których biomasa występuje w formie granul i/lub biofilmu, a także kombinacje tych technologii. Badania oparte na biologii molekularnej sugerują natomiast, że największe zdolności adaptacyjne do niskiej temperatury wykazują bakterie anammox z rodzaju Candidatus Brocadia. W dalszym ciągu wyzwaniem pozostaje jednak utrzymanie stabilnego procesu nitrytacji – anammox w temperaturze 10°C oraz sposób i czas adaptacji biomasy.
Partial nitrification (nitritation) – anammox (anaerobic ammonia oxidation) process is increasingly used to treat wastewater, characterized by a high nitrogen content and high temperature (25–40°C). It is connected with the optimal temperature of anammox bacteria, which is at the range between 30 and 40°C. Mainstream application of anammox for the municipal wastewater, characterized by lower temperature seems to be one of the most challenging, but profitable process. Thenceforth, the research performed in the field of the nitritation – anammox at low temperature (10–20°C) become more and more intense. Compared with the conventional nitrification – denitrification system, nitritation – anammox reduces oxygen demand, eliminates the need for organic carbon source and produces less excess sludge. As a result, it allows to a significant cost reduction. This paper reviews the most important and recent information in the field of nitritation – anammox process at low temperature. Effective nitrogen removal from the municipal wastewater was demonstrated at 15°C in a pilot scale and at 12°C in a laboratory scale reactor. The best performance is achieved in sequencing batch reactors and moving bed reactors with biofilm or granular biomass, as well as combinations of these technologies. Molecular biology studies shows that anammox bacteria of the genus Candidatus Brocadia may have the biggest predispositions to adapt to low temperature. However, temperature about 10°C, time and method of biomass adaptation are still the main challenges for stable and common nitritation – anammox process.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nowoczesne techniki i technologie inżynierii środowiska
Novel Methods and Technologies in Environmental Engineering
Autorzy:
Miksch, K.
Cema, G.
Felis, E.
Sochacki, A.
Tematy:
tlenowe granule
zewnątrzkomórkowe polimery
częściowa nitryfikacja
proces Anammox
zaawansowane procesy utleniania
systemy hybrydowe
oczyszczalnie hydrofitowe
dekoloryzacja barwników
bioremediacja gruntu
mikoryza roślin
techniki biologii molekularne
PCR
FISH
aerobic granules
granule formation
extracellular polymeric substances
partially nitrification
anammox process
advanced oxidation processes
hybrid systems
constructed wetlands
decolorization
synthetic dyes
soil bioremediation
plant mycorrhization
molecular biology techniques
Pokaż więcej
Wydawca:
Politechnika Koszalińska. Wydawnictwo Uczelniane
Powiązania:
https://bibliotekanauki.pl/articles/1818151.pdf  Link otwiera się w nowym oknie
Opis:
The novel technologies used in environmental engineering were discussed in this paper – the formation of aerobic granules, the Anammox process, the advanced oxidation processes, the use of fungi for dyes decolorization, constructed wetlands, the soil phytoremediation supported by rhizosphere microorganisms and the use of molecular biology technique in environmental engineering. The structure of granular sludge is influenced by EPS production. The average diameter and density of biogranules increase due to EPS production. Although polysaccharides are essential, proteins were found to be the predominant component of aerobic granular sludge. Compared to loosely bound EPS (LB-EPS), tightly bound EPS (TB-EPS) showed more significant correlations with granules formation. This investigation will contribute towards a better understanding of the behavior and composition of EPS in sequencing batch reactors. The traditional nitrification and denitrification processes proceed well with typical municipal wastewater. Nevertheless, there are also nitrogen-rich wastewater streams like landfill leachate or reject waters from dewatering of digested sludge, for which traditional nitrification/denitrification can be generally ineffective due to free ammonia inhibition of nitrification and unfavorable biodegradable carbon content for denitrification. Because of high requirements for oxygen and the necessity for addition of external carbon source, treating such nitrogen-rich streams with nitrification/denitrification would become expensive and unsustainable. The least resources consuming pathway for the conversion of ammonium to nitrogen gas is a combination of partial nitrification and the Anammox process. The main advantages of this process compared to the conventional nitrification/denitrification are: low sludge production, decrease of the aeration costs by almost 60% (only half of the ammonia is oxidized to nitrite in the nitritation process without further oxidation to nitrate), and no need for external organic carbon source addition (Anammox process). Furthermore, anammox bacteria oxidize ammonium under anoxic conditions with nitrite as the electron acceptor, and converse energy for CO2 fixation. Additionally, the biomass yield of the Anammox process is very low (0.08 kg VSS kg NH4-N-1 in comparison to 1 kg VSS kg NH4-N-1 in conventional nitrification/denitrification process) consequently, little sludge is produced. The low sludge production is another factor that contributes to the substantially lower operation costs compared to conventional denitrification systems. Advanced oxidation processes (AOPs) are oxidative methods which are based on the generation of the hydroxyl radicals, which are very reactive and less selective than other oxidants. In the wastewater treatment technology, AOPs can be used in a combination with conventional biological techniques (so called hybrid processes), as pre- and post- treatment processes. The advanced oxidation processes have been used in order to increase the biodegradability and also detoxification of the wastewater. The ability of fungi to degrade lignin-cellulose debris is well known. In addition to these natural molecules they may also degrade synthetic compounds, including synthetic dyes. High effectiveness of Evans blue and brilliant green mixture removal by all tested strains was demonstrated. The process was the most effective and fast in shaken conditions. Finally strain MB removed 90% of tested mixture in shaken samples after 96h. It was the best result reached among all the strains used in the experiment. High removal efficiency was accompanied by a decrease of toxicity (from V class to III class in test with D. magna and from IV class even to non-toxic in test with L. minor). The highest decrease of phytotoxicity was noticed in samples with shaken biomass in which the effect of dyes mixture elimination was the best. The research indicates very high potential of tested strains for decolorization and detoxification of dyes mixture. Constructed wetlands are man-made system mimicking the process occurring in natural wetlands. These systems are considered to be an alternative to more technically advanced waste water treatment technologies. The development of constructed wetlands is envisaged to pursue the following directions grouped according to: the type of the waste water to be treated, target contaminants, treatment intensification methods, ancillary benefits and the locality. Mycorrhiza fungi can be used for phytoremediation proccess. They support plant growth by lowering the stress caused by the lack of phosphorus and water. They produce enzymes participating in several stages of xenobiotics decomposition, which is helpful in their further biodegradation performed by the other rhisospherical organisms. The natural colonisation of PAHs contaminated soil is a long-term process. It could be shortend by adding fungal propagules as an inoculum to the soil. Fungi used for the injections should be isolated from PAHs contaminated soil. That guarantees their survival and development in the contaminated environment. The level of PAHs elimination from soil depends on a type of bioremediation modification used. It was shown that the best results are obtained with monocotylous plants combined with bacterial and fungal biopreparations obtained from contaminated soil. The symbiosis of mycorrhiza fungi with monocotylous plants caused ca. 40% increase of 3, 4, 5 and 30% of 6-ring hydrocarbons removal from soil in comparison with the conventional methods. Important aspect of environmental protection and engineering is the possibility for qualitative and quantitative monitoring of complex microbial communities, responsible for biotechnological processes, such as: soil bioremediation, wastewater treatment or composting. Due to the fact that most of the environmental bacteria cannot be grown in the laboratory conditions molecular techniques are widely used in environmental engineering. Among these methods the Polymerase Chain Reaction (PCR)-based and hybridization-based (such as Fluorescent in situ Hybridization; FISH) techniques are known to be the most useful.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of the duration of feeding phase on the genotypic structure of bacterial communities in two sequencing batch reactors treating reject water by partial nitritation and anammox
Autorzy:
Ziembińska-Buczyńska, A.
Cema, G.
Meresta, A.
Płonka, L.
Tematy:
activated sludge
anammox
ammonia
biocenosis
dynamics nitrification
osad czynny
amoniak
biocenoza
dynamika nitryfikacji
Pokaż więcej
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Powiązania:
https://bibliotekanauki.pl/articles/208156.pdf  Link otwiera się w nowym oknie
Opis:
Microbial community and physiochemical processes were monitored by means of denaturing gradient gel electrophoresis (PCR-DGGE) in two sequencing batch reactors (SBRs) running for partial nitritation-anammox and differing in the feeding phase durations (6 h 40 min for SBR1 and 40 min for SBR2). Both SBRs were treated with reject water with a high ammonia concentration (>600 mg for over 370 days. The aim of the experiment was to present the influence of this parameter on total bacterial and anammox bacterial community structure. Molecular analysis revealed that a drastic decrease in influent ammonia concentration to the studied communities caused a change of genotypic structure in their composition. The difference in the reactors working scheme can be the reason for divergence in the community structure though having no drastic influence on its performance and biodiversity level. Feeding time has stronger influence on the genotypic composition of the total bacterial community than on anammox biocenosis.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie metody PCR-DGGE do badania zmienności genotypowej bakterii zasiedlających złoże tarczowe oczyszczające modelowe ścieki koksownicze
Application of PCR-DGGE to study genotypic variability of bacteria inhabiting rotating biological contactors treating synthetic coke wastewater
Autorzy:
Ziembińska-Buczyńska, A.
Cema, G.
Kalbarczyk, M.
Żabczyński, S.
Tematy:
bakterie Anammox
monitoring zmienności genotypowej
ścieki przemysłowe
Anammox bacteria
genotypic monitoring
industrial wastewater
Pokaż więcej
Wydawca:
Polskie Zrzeszenie Inżynierów i Techników Sanitarnych
Powiązania:
https://bibliotekanauki.pl/articles/237434.pdf  Link otwiera się w nowym oknie
Opis:
Ze względu na znaczny ładunek azotu amonowego, którym charakteryzują się ścieki koksownicze, dostrzeżono możliwość wykorzystania procesu beztlenowego utleniania amoniaku (Anammox) w procesie biologicznego oczyszczania tych ścieków. W czasie 8-miesięcznego eksperymentu zbadano bioróżnorodność i zmienność mikroorganizmów błony biologicznej w złożu tarczowym oczyszczającym modelowe ścieki koksownicze, ze szczególnym uwzględnieniem grupy bakterii Anammox. Wykorzystując metodę PCR-DGGE wykazano, że w biofilmie złoża tarczowego funkcjonują bakterie z grupy Anammox. Możliwe, że w trakcie trwania eksperymentu nieznany czynnik środowiskowy (prawdopodobnie nagromadzenie azotanów(III)) spowodował znaczne zubożenie biocenozy bakterii Anammox, powodując jednocześnie zmianę jakościową w strukturze całości konsorcjum bakterii. Pomimo zmian jakościowych poziom bioróżnorodności bakterii w trakcie trwania eksperymentu był względnie stały. W oparciu o uzyskane wyniki można przypuszczać, że polimerazy typu TAQ amplifikują cześć materiału DNA ze złoża tarczowego w sposób niespecyficzny, tj. nieulegający rozdziałowi w gradiencie czynnika denaturującego. Zakłada się konieczność wykorzystania innego typu polimerazy (np. proofreading) i/lub zmiany gradientu DGGE w celu rozwiązania tego problemu.
High ammonia nitrogen load of coke plant wastewater provided an opportunity to use the Anammox (ANaerobic AMmonium OXidation) process for its biological treatment. Biodiversity and variability of microorganisms in rotating biological contactor (RBC) biofilm treating synthetic coke wastewater was analyzed in the eight-month experiment with the main focus on the Anammox bacteria. Using PCR-DGGE (polymerase chain reaction – denaturing gradient gel electrophoresis) it was shown that the RBC biofilm was populated by Anammox bacteria. Possibly, an unknown environmental factor (probably nitrate(III) build-up) caused significant decrease in Anammox bacteria number, leading to a qualitative change in the total bacterial community structure at the same time. However, despite qualitative changes the bacterial biodiversity level remained relatively constant during the course of the experiment. On the basis of the obtained results it may be assumed that TAQ polymerases amplify part of DNA material from the RBC in a non-specific manner and this material is not separated properly in denaturing gradient. Use of other type of polymerase (e.g. proofreading) and/or change of DGGE gradient is presumed necessary as a solution to this problem.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Qualitative analysis of bacterial biocenoses in two sequencing batch reactors treating reject water under different technological conditions
Autorzy:
Karło, A.
Ziembińska-Buczyńska, A.
Cema, G.
Surmacz-Górska, J.
Tematy:
anammox
CANON
nitrification
PCR-DGGE
RT-PCR-DGGE
nitryfikacja
Pokaż więcej
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Powiązania:
https://bibliotekanauki.pl/articles/363132.pdf  Link otwiera się w nowym oknie
Opis:
Complete nitrogen removal over nitrite (CANON) was used to treat reject water with ammonia concentrations ranging from 70 to 154mg·L-1. Two experimental sequential batch reactors, SBR_A and SBR_B, differed in the time of the reject water inflow (6h40min vs 40min), process temperature (25 vs 29°C), and the number of aeration periods per day (3 vs 6, respectively). Nitrogen removal efficiency was higher in SBR_B (50-90%) than in SBR_A (40-80%). Analysis of total (PCR-DGGE) and active (RT-PCR-DGGE) bacteria revealed that the biodiversity of the bacterial biocenoses, expressed as the Shannon-Wiener Biodiversity Index, was higher in SBR_B (2.75-3.10) than in SBR_A (1.80-2.75).
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Start-up, modelling and simulation of the anammox process in a membrane bioreactor
Autorzy:
Cema, G.
Sochacki, A.
Kubiatowicz, J.
Gutwiński, P.
Surmacz-Górska, J.
Tematy:
activate sludge model
anammox
computer simulation
modeling
nitrogen removal
aktywowane modelu osadu
symulacja komputerowa
modelowanie
usuwanie azotu
Pokaż więcej
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Powiązania:
https://bibliotekanauki.pl/articles/185149.pdf  Link otwiera się w nowym oknie
Opis:
There are certain well-known methods of diminishing concentrations of nitrogen compounds, but they are ineffective in case of nitrogen-rich wastewater with a low content of biodegradable carbon. Partial nitritation followed by anaerobic ammonium oxidation (Anammox) process appear to be an excellent alternative for traditional nitrification and denitrification. This paper presents the feasibility of successful start-up of Anammox process in a laboratory-scale membrane bioreactor (MBR). It was shown that the combination of membrane technology and Anammox process allowed to create a new highly efficient and compact system for nitrogen removal. It was possible to achieve average nitrogen removal efficiency equal to 76.7 +/-8.3%. It was shown that the start-up period of 6 months was needed to obtain high nitrogen removal efficiency. The applied biochemical model of the Anammox process was based on the state-of-the-art Activated Sludge Model No.1 (ASM 1) which was modified for accounting activity of autotrophs (nitrite-oxidising bacteria and nitrateoxidising bacteria) and anammox bacteria. In order to increase the predictive power of the simulation selected parameters of the model were adjusted during model calibration. Readjustment of the model parameters based on the critically evaluated data of the reactor resulted in a satisfactory match between the model predictions and the actual observations.
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-6 z 6

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