Autor: Escobar, Carlos - Prolib Integro

Skocz do pozycji: 1.

Autorzy:: Ntaios, George
Solnica, Bogdan
Toth, Peter P.
Sosnowska, Bozena
Gaita, Dan
Latkovskis, Gustavs
Jóźwiak, Jacek
Okopień, Boguslaw
Lewek, Joanna
Penson, Peter E.
Proietti, Marco
Sadowski, Jakub
Escobar, Carlos
Dalakoti, Mayank
Bielecka-Dabrowa, Agata
Banach, Maciej
Covic, Adrian
Pella, Daniel
Cicero, Arrigo F.G.
Lip, Gregory Y.H.
Gaita, Laura
Viigimaa, Margus
Pećin, Ivan
Fogacci, Federica
Ahn, Hyo-Jeong
Dostawca treści:: Repozytorium Uniwersytetu Jagiellońskiego

Artykuł

na półce

Skocz do pozycji: 2.

Tytuł:: Alkaloid production and response to natural adverse conditions in Peganum harmala: in silico transcriptome analyses
Autorzy:: Jazayeri, Seyed Mehdi
Pooralinaghi, Mahtab
Torres-Navarrete, Yenny
Oviedo-Bayas, Byron
Espinoza Guerra, Italo
Herrera Jacome, Dario
Quinaluisa Morán, César
Salas Macias, Carlos
Montes Escobar, Karime
Ghafoor, Seyed Mohammad Hossein Ale Seyed
Veiskarami, Gholamhasan
Jandaghi, Pouria
Villamar Torres, Ronald Oswaldo
Tematy:: SSR
model plant
transcription factor
RNA-Seq
halophyte; Pokaż więcej
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Powiązania:: https://bibliotekanauki.pl/articles/16648150.pdf Link otwiera się w nowym oknie
Opis:: Peganum harmala is a valuable wild plant that grows and survives under adverse conditions and produces pharmaceutical alkaloid metabolites. Using different assemblers to develop a transcriptome improves the quality of assembled transcriptome. In this study, a concrete and accurate method for detecting stress-responsive transcripts by comparing stress-related gene ontology (GO) terms and public domains was designed. An integrated transcriptome for P. harmala including 42 656 coding sequences was created by merging de novo assembled transcriptomes. Around 35 000 transcripts were annotated with more than 90% resemblance to three closely related species of Citrus, which confirmed the robustness of the assembled transcriptome; 4853 stress responsive transcripts were identified. CYP82 involved in alkaloid biosynthesis showed a higher number of transcripts in P. harmala than in other plants, indicating its diverse alkaloid biosynthesis attributes. Transcription factors (TFs) and regulatory elements with 3887 transcripts comprised 9% of the transcriptome. Among the TFs of the integrated transcriptome, cystein2/histidine2 (C2H2) and WD40 repeat families were the most abundant. The Kyoto Encyclopedia of Genes and Genomes (KEGG) MAPK (mitogen-activated protein kinase) signaling map and the plant hormone signal transduction map showed the highest assigned genes to these pathways, suggesting their potential stress resistance. The P. harmala whole-transcriptome survey provides important resources and paves the way for functional and comparative genomic studies on this plant to discover stress-tolerance-related markers and response mechanisms in stress physiology, phytochemistry, ecology, biodiversity, and evolution. P. harmala can be a potential model for studying adverse environmental cues and metabolite biosynthesis and a major source for the production of various alkaloids.
Dostawca treści:: Biblioteka Nauki