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Wyszukujesz frazę "Fitzner, A." wg kryterium: Autor


Wyświetlanie 1-4 z 4
Tytuł:
Skuteczność szczepień przeciwko krwotocznej chorobie królików (RHD) w kontekście zmienności genetycznej i antygenowej wirusa
The effectiveness of vaccination against rabbit haemorrhagic disease (RHD) in the context of the genetic and antigenic variability of the virus
Autorzy:
Fitzner, A.
Niedbalski, W.
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Powiązania:
https://bibliotekanauki.pl/articles/860417.pdf  Link otwiera się w nowym oknie
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serological survey for RHD antibodies in rabbits from two types of rabbit breeding farms
Autorzy:
Fitzner, A.
Niedbalski, W.
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/30977.pdf  Link otwiera się w nowym oknie
Opis:
Seroprevalence studies of RHDV antibodies in domestic rabbits were conducted between 2008-2014. A total of 12,169 sera from the provinces of central, southern and south-east Poland, including 7,570 samples collected from mixed-breed rabbits reared in smallholder farms and nearly 4,600 sera taken mainly from unvaccinated rabbits kept in industrial farms, were examined using ELISA tests. Additionally, cross-reactivity of selected tested and control archival sera using both classic RHDV and RHDVa antigens was determined by HI assay. The overall seroprevalence was 13.3%. In rabbits with unkown history of immunisation or RHD infection which came from small farms, RHDV antibodies were detected in 6.1% ranging between 1.0% to 17.2% of animals. In rabbits of the same group, but with a declared vaccination status, or confirmed exposure to an infectious virus, or coming from exposed females, the seroprevalence ranged from 83% to 100%. Among unvaccinated meat rabbits aged 71 to 90 days from industrial farms, low (1.85%, 4.17%, 11%), medium (34%, 54%) or high rates (98.7%) of seropositivity were detected. The seroconversion recorded in adult vaccinated females from industrial farms was 70% and 95%. Generally, the antibody levels examined by ELISAs and HI were comparable. However, a number of sera from the rabbits from small farms, as well as archival sera, showed clear differences. Several-fold differences in antibody titers, evidenced mainly in the postoutbreak sera, indictaed the contact of animals with RHDVa antigen. The overall results of the survey revealed a great proportion of seronegative rabbits potentially highly susceptible to RHD infection. In combination with the emergence of a novel pathogenic RHD virus type (RHDV2), it poses a severe risk of a next wave of fatal disease cases spreading in the native population of domestic rabbits, especially in farms with a traditional system of husbandry.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Polish RHDVa subtype strains based on the analysis of a highly variable part VP60 gene
Autorzy:
Fitzner, A.
Niedbalski, W.
Paprocka, G.
Kesy, A.
Tematy:
identification
RHD virus
genetic variability
Polska
virus subtype strain
genetic variant
sequence analysis
VP60 gene
rabbit
nucleotide sequence
phylogenetic analysis
hemorrhagic disease
virus strain
Pokaż więcej
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/31138.pdf  Link otwiera się w nowym oknie
Opis:
In order to determine the genetic variability of Polish RHD virus strains and to confirm the presence of genetic variant (RHDVa) subtype the partial nucleotide sequences of capsid protein gene, including two highly variable regions C and E, were examined. Phylogenetic analyses of 15 viral strains obtained over 18 years revealed the presence of three genetic groups. The oldest RHDV strains exhibit very close amino acid sequence similarity (98-99%) to the German FRG89 reference strain and most of European strains of the same period, as well as Chinese isolate from 1984. The HA-negative strains and isolates with variable reactivity in the HA test belong to the second subgroup and exhibit an intermediate level of variability (about 3%) in the analysed VP60 gene fragment. The most genetically variable strains (6-7%) clustered to RHDVa subtype. The analysis of nucleotides and amino acid sequences demonstrated three pairs of well conserved RHDV strains, isolated over 3, 6 and 10-year period.
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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