- Tytuł:
- Sample Preparation Followed by High Performance Liquid Chromatographic (HPLC) Analysis for Monitoring Muconic Acid as a Biomarker of Occupational Exposure to Benzene
- Autorzy:
-
Shahtaheri, S. J.
Ghamari, F.
Golbabaei, F.
Rahimi-Froushani, A.
Abdollahi, M. - Tematy:
-
benzene
muconic acid
sample preparation
solid-phase extraction
biological monitoring
chromatography - Pokaż więcej
- Wydawca:
- Centralny Instytut Ochrony Pracy
- Powiązania:
- https://bibliotekanauki.pl/articles/91059.pdf Link otwiera się w nowym oknie
- Opis:
- Factors affecting solid phase extraction (SPE) of trans,trans-muconic acid (ttMA), as a benzene biomarker, including sample pH, sample concentration, sample volume, sample flow rate, washing solvent, elution solvent, and type of sorbent were evaluated. Extracted samples were determined by HPLC-UV (high performance liquid chromatography-ultraviolet). The analytical column was C18, UV wave length was 259 nm, and the mobile phase was H2O/methanol/acetic acid run at flow rate of 1 ml/min. A strong anion exchange silica cartridge was found successful in simplifying SPE. There was a significant difference between recoveries of ttMA when different factors were used (p < .001). An optimum recovery was obtained when sample pH was adjusted at 7. There was no significant difference when different sample concentrations were used (p > .05). The optimized method was then validated with 3 different pools of samples showing good reproducibility over 6 consecutive days and 6 within-day experiments.
- Dostawca treści:
- Biblioteka Nauki
Artykuł