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    Wyświetlanie 1-2 z 2
    Tytuł:
    Data of the Insect Biome Atlas : a metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar
    Autorzy:
    Prus-Frankowska, Monika
    Lindholm, L.-T.
    Mertz, L.
    Kanerot, S.
    Skord, J.
    Martinelli, J.
    Patchett, A.
    Karlsson, A.
    Rajoelison, E. T.
    Gunnarsson, M.
    Hed, A.
    Lundin, E.
    Regazzoni, E.
    Szpryngiel, S.
    Stålhandske, T.
    Rosander, K.-G.
    Lundberg, J.
    Aspebo, M.
    Fahlen, L.
    Alberg, L.
    Karlsson, D.
    Fürst, P.
    Emanuelsson, H.
    Desborough, L.
    Eliasson, A.
    Vilhelmsson, J.
    Dolff, E.
    Sundh, J.
    Tyboni, M.
    Åström, S.
    Fisher, B. L.
    Bergman, P.-E.
    Kraft, T.
    Persson, N.
    Nilsson, J.
    Tajani, K.
    Raharinjanahary, D.
    Vestermark, L.
    Wass, A.
    Persson, J.
    Jönsson, A.
    Randrianandrasana, C.
    Deng, Junchen
    Ugarph, C.
    Guasconi, D.
    Åhlén, D.
    Ljung, K.
    Wetterstrand, P.
    Klinth, M.
    Łukasik, Piotr
    Iwaszkiewicz-Eggebrecht, E.
    Ljung, U.
    Bellviken, A.
    Erixon, R.
    Ranaivo, C.
    Lindholm, M.
    Miraldo, A.
    Manoharan, L.
    Grudd, H.
    Hörnström, E.
    Andersson, A. F.
    Sandblom, C.
    Frogner, C.
    Goodsell, R.
    Wahlgren, N.
    Marquina, D.
    Lernefalk, H.
    Björkman, M. P.
    Lahti, R.
    Elmquist, H.
    Granqvist, E.
    Buczek, Mateusz
    Rydgård, M.
    Larsson, M.
    Malmenius, M.
    Roslin, T.
    Häggqvist, S.
    Svensson, F.
    van Dijk, L. J. A.
    Ronquist, F.
    Johansson, H.
    Tack, A. J. M.
    Grabs, A.
    Björklund, S.
    Lestander, Y.
    Rafanomezantsoa, J.-J.
    Opis:
    We present the data from the Insect Biome Atlas project (IBA), characterizing the terrestrial arthropod faunas of Sweden and Madagascar. Over 12 months, Malaise trap samples were collected weekly (biweekly or monthly in the winter, when feasible) at 203 locations within 100 sites in Sweden and weekly at 50 locations within 33 sites in Madagascar; this was complemented by soil and litter samples from each site. The field samples comprise 4,749 Malaise trap, 192 soil and 192 litter samples from Sweden and 2,566 Malaise trap and 190 litter samples from Madagascar. Samples were processed using mild lysis or homogenization, followed by DNA metabarcoding of CO1 (418 bp). The data comprise 698,378 non-chimeric sequence variants from Sweden and 687,866 from Madagascar, representing 33,989 (33,046 Arthropoda) and 77,599 (77,380 Arthropoda) operational taxonomic units, respectively. These are the most comprehensive data presented on these faunas so far, allowing unique analyses of the size, composition, spatial turnover and seasonal dynamics of the sampled communities. They also provide an invaluable baseline against which to gauge future changes.
    Dostawca treści:
    Repozytorium Uniwersytetu Jagiellońskiego
    Artykuł
    Tytuł:
    High-throughput biodiversity surveying sheds new light on the brightest of insect taxa
    Autorzy:
    van Dijk, Laura J. A.
    Roslin, Tomas
    Goodsell, Robert M.
    Tack, Ayco Jerome Michel
    Iwaszkiewicz-Eggebrecht, Ela
    Ronquist, Fredrik
    Andersson, Anders
    Klinth, Mårten
    Mutanen, Marko
    Łukasik, Piotr
    Bengsson, Bengt-Åke
    Miraldo, Andreia
    Opis:
    DNA metabarcoding of species-rich taxa is becoming a popular high-throughput method for biodiversity inventories. Unfortunately, its accuracy and efficiency remain unclear, as results mostly pertain to poorly known taxa in underexplored regions. This study evaluates what an extensive sampling effort combined with metabarcoding can tell us about the lepidopteran fauna of Sweden - one of the best-understood insect taxa in one of the most-surveyed countries of the world. We deployed 197 Malaise traps across Sweden for a year, generating 4749 bulk samples for metabarcoding, and compared the results to existing data sources. We detected more than half (1535) of the 2990 known Swedish lepidopteran species and 323 species not reported during the sampling period by other data providers. Full-length barcoding confirmed three new species for the country, substantial range extensions for two species and eight genetically distinct barcode variants potentially representing new species, one of which has since been described. Most new records represented small, inconspicuous species from poorly surveyed regions, highlighting components of the fauna overlooked by traditional surveying. These findings demonstrate that DNA metabarcoding is a highly efficient and accurate biodiversity sampling method, capable of yielding significant new discoveries even for the most well known of insect faunas.
    Dostawca treści:
    Repozytorium Uniwersytetu Jagiellońskiego
    Artykuł
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