Autor: Koglin, Jason - Prolib Integro

Skocz do pozycji: 2.

Tytuł:: Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
Autorzy:: Nogły, Przemysław
Kimura, Tetsunari
Coe, Jesse
Dods, Robert
Iwata, So
Schertler, Gebhard
Harimoorthy, Rajiv
Standfuss, Jörg
Rheinberger, Jan
James, Daniel
Zhao, Yun
Spence, John
Frank, Matthias
Hunter, Mark S.
Sala, Leonardo
Barty, Anton
Wu, Wenting
Beyerlein, Kenneth R.
DePonte, Daniel
Panneels, Valerie
White, Thomas A.
Conrad, Chelsie
Milathianaki, Despina
Kubo, Minoru
Li, Chufeng
Williams, Garth J.
Båth, Petra
Weierstall, Uwe
Neutze, Richard
Boutet, Sébastien
Berntsen, Peter
Gati, Cornelius
Milne, Christopher
Koglin, Jason E.
Chapman, Henry N.
Nelson, Garrett
Abela, Rafael
Fromme, Petra
Nango, Eriko
Bean, Richard
Opis:: Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
Dostawca treści:: Repozytorium Uniwersytetu Jagiellońskiego

Artykuł

na półce

Informacja

Wyszukujesz frazę "Koglin, Jason" wg kryterium: Autor