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Wyświetlanie 1-10 z 59
Tytuł:
Wpływ chromu (III) na metabolizm kwasów tłuszczowych oraz ekspresję genów szlaku insulinowego w komórkach mięśniowych myszy linii C2C12
The effect of chromium (III) on fatty acid metabolism and insulin path related gene expression in mouse myocytes cell line C2C12
Autorzy:
Lewicki, S.
Rattman, D.
Kuryl, T.
Snochowski, M.
Debski, B.
Tematy:
chlorek chromu
chrom
czlowiek
ekspresja genow
jony chromu
komorki zwierzece
kwasy tluszczowe
metabolizm
miesnie
myszy
pikolinian chromu
zwierzeta
Pokaż więcej
Wydawca:
Polskie Towarzystwo Technologów Żywności
Powiązania:
https://bibliotekanauki.pl/articles/825955.pdf  Link otwiera się w nowym oknie
Opis:
Jony chromu (III) mają istotny wpływ na przemianę węglowodanowo-lipidową ludzi i zwierząt, powodując u chorych na cukrzycę wzrost wrażliwości komórek na insulinę, zwiększenie przemian kwasów tłuszczowych, spadek masy ciała i poziomu wolnych kwasów tłuszczowych w surowicy. Celem pracy było zbadanie działania chromu na proces beta-oksydacji kwasów tłuszczowych oraz zmiany ekspresji genów szlaku insulinowego w tkance mięśniowej. Do badań użyto mysich komórek mięśniowych linii C2C12 poddanych 4-dniowemu różnicowaniu. Chrom dodawano do medium w postaci chlorku lub pikolinianu chromu a aktywność β-oksydacji mierzono po 1, 3, 6 lub 48 godzinnej inkubacji. Suplementacja chromem w stężeniu 1 μgCr³⁺/L spowodowała wzrost (p < 0,001) aktywności procesu spalania kwasów tłuszczowych w 1, 3 godzinie inkubacji z pikolinianem oraz w 1, 3, i 6 godzinie inkubacji z chlorkiem chromu. Po 48 godzinnej inkubacji z jonami chromu obserwowano obniżenie aktywności tego procesu. Wpływ chlorku chromu 10 μgCr³⁺/L na ekspresję genów zaangażowanych w szlak przekazywania sygnału od insuliny określono z wykorzystaniem mikromacierzy SuperArray. Chrom po 4 godz. inkubacji spowodował wzrost ekspresji 22 genów natomiast po 24 godzinach wzrost dwóch i spadek ekspresji dwóch genów w porównaniu z kontrolą. Uzyskane wyniki wskazują na pozytywny wpływ suplementacji chromem na zwiększenie aktywności β-oksydacji. Dane uzyskane techniką mikromacierzy wskazują na interakcję jonów chromu ze szlakiem przewodzenia sygnału insulinowego również na poziomie transkrypcyjnym. Wzrost ekspresji genów związanych z metabolizmem lipidów i genów docelowych dla PPAR sugerują trwałe efekty wywołane przez jony chromu.
Chromium (III) ions influence significantly carbohydrate-lipid metabolism causing in diabetic subjects an optimalisation of insulin action, increase of lipid alteration, decrease body weight and free fatty acids plasma level. The aim of the present study was to describe changes in β-oxidation of fatty acids and gene expression following chromium supplementation. The experiments were performed in mouse myoblast C2C12 cell line over 4 days differentiation. Chromium was added to medium (DMEM), as chromium chloride (CrCl₃) or chromium picolinate, in 1 and 10 μgCr³⁺/L concentrations. Beta-oxidation of fatty acids activity was measured after 1, 3, 6, or 48 h. incubations. Introduction of chromium 1 μgCr³⁺/L to cell culture resulted intensification of fatty acids oxidation after 1 and 3 h (picolinate, p < 0,001) and 1, 3, and 6 h (chloride, p < 0,001) incubation. We observed higher stimulation effect along chromium chloride administration (about 50% of control value) than chromium picolinate (25% of control value). After 48h incubation decrease of fatty acids oxidation were noticed. The influence of chromium chloride (10 μgCr³⁺/L) supplementation on gene expression was examined using microarray SuperArray technique. Chromium added caused increase in 22 genes expression over 4h while only 2 increase and 2 decrease over 24h incubation in compare with control. The results in these studies showed positive effect of chromium supplementation on activity of β-oxidation. Results from microarray analysis indicate chromium interaction on signaling insulin pathway, also on transcription level. Increased expression of genes engaged in lipid metabolism and genes activated by peroxisome proliferator-activated receptor PPAR suggest permanent effect caused by chromium ions.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Feeding pregnant and lactating mice Rhodiola kirilowii extracts helps to preserve thymus function of their adult progeny
Autorzy:
Bien, K.
Lewicki, S.
Zdanowski, R.
Skopinska-Rozewska, E.
Krzyzowska, M.
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/30870.pdf  Link otwiera się w nowym oknie
Opis:
Plants belonging to Rhodiola kirilowii species, members of Rhodiola genus and Crassulaceae family, grow wildly in Tibet, Mongolia and China mountains and are traditionally used as adaptogens, antidepressants and anti-inflammatory remedies. Nowadays, R. kirilowii is cultivated in some countries, also in Poland. In our previous papers we reported immuno- and angio-modulatory effects of aqueous and hydro-alcoholic extracts of radix and rhizome of this plant in non-pregnant and pregnant mice. The aim of the present study was to evaluate the effect of feeding pregnant and further lactating mice these extracts on selected thymus function parameters in adult progeny. The counts of M-30+ apoptotic cells, in the thymuses obtained from progeny of mice fed during pregnancy and lactation water or 50% water-alcoholic extract of Rhodiola kirilowii, were significantly lower (p<0.05) than apoptotic cells counts observed in the control mice. No significant differences in the counts of IL-7-positive cells in the thymuses obtained from progeny of the control mice and mothers treated with water or hydro-alcohol extracts of Rhodiola kirilowii were observed.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vivo immuno - and angiomodulatory effects of Aloe arborescens folii recentis extractum siccum (AAES) in mice
Autorzy:
Zdanowski, R.
Bałan, B.J.
Schonknecht, K.
Skopiński, P.
Stelmasiak, M.
Skopińska-Różewska, E.
Lewicki, S.
Tematy:
Aloe arborescens
herbal medicinal product
Biostymina preparation
Phytopharm Kleka
water extract
antibody response
angiogenesis
mice
mouse
wyciąg wodny z Aloe arborescens
myszy
splenocyty
angiogeneza (test LIA)
proliferacja komórek (PHA)
produkcja przeciwciał
Pokaż więcej
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Powiązania:
https://bibliotekanauki.pl/articles/2049078.pdf  Link otwiera się w nowym oknie
Opis:
Introduction: AAES is a powdered form of Biostymina, herbal medicinal product of Phytopharm Klęka S.A., a water extract of Aloe arborescens Mill. leaves. Aloe arborescens Mill. (woody aloe, tree-like aloe) is known to have several traditional medicinal properties including anti-inflammatory, immunomodulatory, antiviral and antimicrobial activity. Objective: The aim of this work was to study the in vivo effect of AAES on cellular (leukocyte-induced cutaneous angiogenesis, LIA test, and proliferative response to PHA) and humoral (anti-SRBC antibody response)immunity in mice. Methods: Balb/c mice were fed AAES from 0.5 to 75 mg/kg body mass for seven days before grafting their splenocytes intradermally to F1 (Balb/cxC3H) recipients (LIA test). Neovascular reaction was evaluated 72 h later in dissection microscope. Spleen cell cultures were incubated with 0.5, 1 and 2 µg/ml of PHA. After 48 h of incubation, tritiated thymidine was added. After further 24 h, cells were harvested (Skatron) and incorporation of tritiated thymidine was measured using Beta-scintillation counter. Balb/c mice were fed for 7 days with AAES, then immunized intraperitoneally with 5% SRBC suspension and 7 days later the antibody response was measured with hemagglutination test. Results: Neovascular reaction was significantly higher in groups grafted with splenocytes collected from all AAES fed donors than from the controls. The proliferation of splenocytes taken from mice fed AAES at doses ranging from 0.5 mg/kg to 7.5 mg/kg was stimulated in all cultures. Suppression of proliferation was observed in cell cultures derived from mice fed with higher doses of AAES. Stimulation of anti-SRBC antibody production was seen in mice fed both 2.5 and 7.5 mg/kg dose of AAES. Conclusion: Powdered form of Biostymina (AAES) might be useful in the treatment of patients with ischaemia of tissues and organs (myocardial infarction, stroke, necrosis) and in deficiency in the production of immune cells and growth factors (infections, chronic wound healing, ulceration and bone fusion).
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphometric abnormalities in the spleen of the progeny of mice fed epigallocatechin during gestation and nursing
Autorzy:
Balan, B.J.
Skopinska-Rozewska, E.
Skopinski, P.
Zdanowski, R.
Lesniak, M.
Kiepura, A.
Lewicki, S.
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/31390.pdf  Link otwiera się w nowym oknie
Opis:
It is very difficult to cure pregnant females suffering from infections, because of the risk which might occur during treatment by several, even herbal, medications. Many of these substances, among them extracts from plants, have antimicrobial, anti-inflammatory and immunostimulatory properties owing to their polyphenols content, but also may reveal unwanted effects on the fetal development because of their anti-angiogenic properties. The aim of the present study was to elucidate whether daily feeding pregnant and nursing mice 0.2 mg/kg epigallocatechin (EGC), previously recognized as angiogenesis inhibitor, may lead to abnormalities in morphology of spleen and in some parameters of immune function of their adult, 6-week old progeny. Morphometry of EGC offspring spleens revealed lower number of lymphatic nodules and their larger diameter than those found in the control offspring. Cellularity of spleens was lower in EGC offspring than in the controls. Cytometric analysis showed that this decline concerns lymphocytes with CD335 (p<0.001), CD19 (p<0.01) and CD4 (p<0.05) markers. No differences were observed in the humoral response to the immunization with SRBC, and in the proliferative response of splenocytes to mitogens PHA, ConA and LPS.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vivo inhibitory effect of Aloe vera gel on the ability of mouse parental splenic lymphocytes to induce cutaneous angiogenesis in recipient F1 mice
Autorzy:
Skopinski, P.
Lewicki, S.
Balan, B.J.
Kocik, J.
Zdanowski, R.
Skopinska-Rozewska, E.
Siwicki, A.K.
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/30546.pdf  Link otwiera się w nowym oknie
Opis:
Lymphocyte-induced angiogenesis test (LIA) is a model of local graft-versus-host (GVH) reaction, marker of the earliest events resulting from activation of donor lymphocytes after contact with host semi-allogeneic histocompatibility antigens. The effect of in vivo oral administration of Aloe vera gel for 21 days to maternal strain (Balb/c) donor mice on the ability of their splenic lymphocytes to induce cutaneous angiogenesis (LIA test) in F1 Balb/c x C3H recipients, was studied. Results: Neovascular reaction evaluated 72 hours after cells grafting was significantly lower in F1 mice grafted with lymphocytes collected from Aloe- fed donors, than in recipients of lymphocytes collected from respective controls. Conclusions: This observation opens the promise of safe and ethically acceptable possibility of use of Aloe vera gel in human donors in prevention of GVHD in recipients of bone marrow grafts.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphometric abnormalities in spleen and kidney of the progeny of mice fed American cranberry extract (Vaccinium macrocarpon) during pregnancy and lactation
Autorzy:
Balan, B.J.
Lewicki, S.
Siwicki, A.K.
Stelmasiak, M.
Skopinski, P.
Skopinska-Rozewska, E.
Wasiutynski, A.
Zdanowski, R.
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/30729.pdf  Link otwiera się w nowym oknie
Opis:
Cranberries and cranberry-derived diet supplements are often recommended for the treatment of urinary tract infections, also during pregnancy. These products contain strongly anti-angiogenic chemical compounds which could not be indifferent to the developing fetus. In the present work we evaluated the effect of feeding pregnant and lactating mice American cranberry extract (daily dose 0.88 mg) on the morphology and some parameters of spleen and kidney function of their adult progeny. Six weeks after delivery the morphometry of spleen and kidney, cytometric analysis of spleen lymphocytes, evaluation of humoral response to SRBC (Sheep Red Blood Cells), and examination of serum creatinine/urea concentration, were performed in the offspring. Spleens of progeny from experimental (E) group differed from the spleens of progeny of control mice in the lower number of lymphatic nodules and their larger diameter. Cytometry of spleen cells from progeny of E mothers revealed more CD19⁺ and CD8⁺ lymphocytes than in the control group. No difference was seen in the response to immunization by red blood cells of sheep (SRBC) between control and E offspring. An increase in the diameter of glomeruli was observed in the kidneys of the experimental group in comparison with the control group. No abnormalities in creatinine and urea serum level were observed. A higher concentration of VEGF and bFGF in E offspring sera in comparison to the controls was seen. Conclusion: Although the observed differences between the control and experimental group were not large, caution is recommended in using cranberries and their extracts during pregnancy until more research will be done on this topic.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spleen content of selected polyphenols, splenocytes morphology and function in mice fed Rhodiola kirilowii extracts during pregnancy and lactation
Autorzy:
Lewicki, S.
Stankiewicz, W.
Skopinska-Rozewska, E.
Wilczak, J.
Lesniak, M.
Suska, M.
Siwicki, A.K.
Skopinski, P.
Zdanowski, R.
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/31572.pdf  Link otwiera się w nowym oknie
Opis:
The genus Rhodiola (Crassulaceae) consists of many species, growing mainly in Asia and traditionally used as adaptogens and anti-inflammatory drugs. In order to elaborate herbal immunostimulator which could be safely given to pregnant women, we performed a study on immunotropic effects of feeding pregnant and lactating mice Rhodiola kirilowii extracts. This paper presents the results of the first part of our study – spleen content of selected polyphenols, spleen cellularity, splenocytes phenotype and their response to mitogens. Experiments were performed on adult inbred females of Balb/c strain, mated with adult males. Females, since copulatory plug was noted, up to the 28-th day after delivery were fed daily with 20 mg/kg b.m. water (RKW) or hydro- lcoholic (RKW-A) extracts of Rhodiola kirilowii. Results: 1. Significantly lower proportion of pregnant mice in experimental groups than in the control. 2. Cellularity of spleen and flavonol quercetin spleen concentration were significantly lower in experimental groups in comparison to the controls. 3. Flavanols ( (+)-catechin and epicatechin) levels were significantly higher in the spleens of experimental mice than in the controls. 4. Positive correlation between spleen cellularity and quercetin, and negative correlation between spleen cellularity and epicatechin content were observed. 5. Spleen mass and spleen lymphocytes phenotype and proliferation in RKW and RKW-A fed mice did not differ from the control. These results, together with suspicion of some embryo- oxicity, are worrying and eliminate the possibility of use Rhodiola kirilowii extracts for long-term treatment in pregnant females.
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 59

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