- Tytuł:
- Spatiotemporal transcriptomic profiling reveals the dynamic immunological landscape of alveolar echinococcosis
- Autorzy:
-
Xu, Xun
Liao, Sha
Chen, Qi
Jin, Xin
Fu, Bao‐Quan
Zhou, Ting‐Ting
Cai, Huimin
You, Jie
Li, Junhua
He, Xiuju
Tian, Xue‐Qi
Li, Jiandong
Wu, Yao‐Dong
Cai, Xue‐Peng
Ou, Zhihua
Tan, Qiming
Zhang, Qian
Zhao, Yanping
Chen, Jialing
He, Fan
Jia, Wan‐Zhong
Liang, Yingan
Chen, Ao
Li, Wenhui
Li, Li
Wu, Yan‐Tao
Yang, Huanming
Li, Xiu‐Rong
Zhang, Nian‐Zhang
Wang, Jian
Han, Xiumin
Hu, Guohai
Sun, Jiwen
Yan, Hong‐Bin
Ren, Peidi
Chen, Wei‐Gang - Opis:
- Alveolar echinococcosis (AE) is caused by the chronic infection of E. multilocularis, whose tumor-like growth can lead to high fatality if improperly treated. The early diagnosis of infection and the treatment of advanced AE remain challenging. Herein, bulk RNA-seq, scRNA-seq, and spatial transcriptomics technologies are integrated, to reveal the host immune response mechanism against E. multilocularis both spatially and chronologically, collecting mouse liver samples at multiple timepoints up to 15 months post infection. These results unveil an unprecedented high-resolution spatial atlas of the E. multilocularis infection foci and the functional roles of neutrophils, $Spp1^{+}$ macrophages, and fibroblasts during disease progression. The heterogeneity of neutrophil and macrophage subpopulations are critical in both parasite-killing and the occurrence of immunosuppression during AE progression. These findings indicate the transition of parasite control strategy from “active killing” to “negative segregation” by the host, providing instructive insights into the treatment strategy for echinococcosis.
- Dostawca treści:
- Repozytorium Uniwersytetu Jagiellońskiego
Artykuł