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    Wyświetlanie 1-5 z 5
    Tytuł:
    Phenytoin decreases pain-like behaviors and improves opioid analgesia in a rat model of neuropathic pain
    Autorzy:
    Ciapała, Katarzyna
    Mika, Joanna
    Kocot-Kępska, Magdalena
    Makuch, Wioletta
    Dobrogowski, Jan
    Pawlik, Katarzyna
    Przeklasa-Muszyńska, Anna
    Zajączkowska, Renata
    Opis:
    Neuropathic pain remains a clinical challenge due to its complex and not yet fully understood pathomechanism, which result in limited analgesic effectiveness of the management offered, particularly for patients with acute, refractory neuropathic pain states. In addition to the introduction of several modern therapeutic approaches, such as neuromodulation or novel anti-neuropathic drugs, significant efforts have been made in the repurposing of well-known substances such as phenytoin. Although its main mechanism of action occurs at sodium channels in excitable and non-excitable cells and is well documented, how the drug affects the disturbed neuropathic interactions at the spinal cord level and how it influences morphine-induced analgesia have not been clarified, both being crucial from a clinical perspective. We demonstrated that single and repeated systemic administrations of phenytoin decreased tactile and thermal hypersensitivity in an animal model of neuropathic pain. Importantly, we observed an increase in the antinociceptive effect on thermal stimuli with repeated administrations of phenytoin. This is the first study to report that phenytoin improves morphine-induced antinociceptive effects and influences microglia/macrophage activity at the spinal cord and dorsal root ganglion levels in a neuropathic pain model. Our findings support the hypothesis that phenytoin may represent an effective strategy for neuropathic pain management in clinical practice, particularly when combination with opioids is needed.
    Dostawca treści:
    Repozytorium Uniwersytetu Jagiellońskiego
    Artykuł
    Tytuł:
    Gravity-driven microfluidic assay for digital enumeration of bacteria and for antibiotic susceptibility testing
    Autorzy:
    Postek, Witold
    Makuch, Karol
    Guzowski, Jan
    Garstecki, Piotr
    Zengerle, Roland
    Ruszczak, Artur
    Kao, Yu-Ting
    Kaminski, Tomasz S.
    von Stetten, Felix
    Wydawca:
    Royal Society of Chemistry
    Cytata wydawnicza:
    Lab Chip, 2020, 20, 54-63
    Opis:
    The alarming dynamics of antibiotic-resistant infections calls for the development of rapid and point-of-care (POC) antibiotic susceptibility testing (AST) methods. Here, we demonstrated the first completely stand-alone microfluidic system that allowed the execution of digital enumeration of bacteria and digital antibiograms without any specialized microfluidic instrumentation. A four-chamber gravity-driven step emulsification device generated ∼2000 monodisperse 2 nanoliter droplets with a coefficient of variation of 8.9% of volumes for 95% of droplets within less than 10 minutes. The manual workload required for droplet generation was limited to the sample preparation, the deposition into the sample inlet of the chip and subsequent orientation of the chip vertically without an additional pumping system. The use of shallow chambers imposing a 2D droplet arrangement provided superior stability of the droplets against coalescence and minimized the leakage of the reporter viability dye between adjacent droplets during long-term culture. By using resazurin as an indicator of the growth of bacteria, we were also able to reduce the assay time to ∼5 hours compared to 20 hours using the standard culture-based test.
    Dostawca treści:
    Repozytorium Centrum Otwartej Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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