Autor: Nicholas, Ben - Prolib Integro

Skocz do pozycji: 1.

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Skocz do pozycji: 2.

Tytuł:: Stratification of asthma phenotypes by airway proteomic signatures
Autorzy:: Nicholas, Ben
Lefaudeux, Diane
Horvath, Ildiko
Riley, John
Dahlen, Sven-Erik
Sun, Kai
Sandstrom, Thomas
Bakke, Per S.
Bansal, Aruna T.
Djukanovic, Ratko
Sousa, Ana R.
Montuschi, Paolo
Sanak, Marek
Chung, Kian Fan
Lutter, Rene
Krug, Norbert
Auffray, Charles
Caruso, Massimo
Xian, Yang
Staykova, Doroteya
Shaw, Dominick E.
Pandis, Ioannis
Corfield, Julie
De Meulder, Bertrand
Folisi, Caterina
Howarth, Peter
Adcock, Ian M.
Rowe, Anthony
Sterk, Peter J.
Wilson, Susan
Guo, Yike
Burg, Dominic
Skipp, Paul J.
Strazzeri, Fabio
Ward, Jonathan
Brandsma, Joost
Schofield, James P.R.
Fowler, Stephen J.
Opis:: neutrophils
proteomics
biomarkers
asthma
Background: Stratification by eosinophil and neutrophil counts increases our understanding of asthma and helps target therapy, but there is room for improvement in our accuracy in prediction of treatment responses and a need for better understanding of the underlying mechanisms. Objective: We sought to identify molecular subphenotypes of asthma defined by proteomic signatures for improved stratification. Methods: Unbiased label-free quantitative mass spectrometry and topological data analysis were used to analyze the proteomes of sputum supernatants from 246 participants (206 asthmatic patients) as a novel means of asthma stratification. Microarray analysis of sputum cells provided transcriptomics data additionally to inform on underlying mechanisms. Results: Analysis of the sputum proteome resulted in 10 clusters (ie, proteotypes) based on similarity in proteomic features, representing discrete molecular subphenotypes of asthma. Overlaying granulocyte counts onto the 10 clusters as metadata further defined 3 of these as highly eosinophilic, 3 as highly neutrophilic, and 2 as highly atopic with relatively low granulocytic inflammation. For each of these 3 phenotypes, logistic regression analysis identified candidate protein biomarkers, and matched transcriptomic data pointed to differentially activated underlying mechanisms. Conclusion: This study provides further stratification of asthma currently classified based on quantification of granulocytic inflammation and provided additional insight into their underlying mechanisms, which could become targets for novel therapies.
eosinophils
Dostawca treści:: Repozytorium Uniwersytetu Jagiellońskiego

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Skocz do pozycji: 3.

Tytuł:: Adaptive responses to cool climate promotes persistence of a non-native lizard
Autorzy:: Halliwell, Ben
Horváthová, Terézia
Michaelides, Sozos
Fresnillo, Belén
Beeton, Nicholas J.
Williamson, Joseph
Uller, Tobias
Prescott, Graham
While, Geoffrey M.
Opis:: Successful establishment and range expansion of non-native species often require rapid accommodation of novel environments. Here, we use common-garden experiments to demonstrate parallel adaptive evolutionary response to a cool climate in populations of wall lizards (Podarcis muralis) introduced from southern Europe into England. Low soil temperatures in the introduced range delay hatching, which generates directional selection for a shorter incubation period. Non-native lizards from two separate lineages have responded to this selection by retaining their embryos for longer before oviposition-hence reducing the time needed to complete embryogenesis in the nest-and by an increased developmental rate at low temperatures. This divergence mirrors local adaptation across latitudes and altitudes within widely distributed species and suggests that evolutionary responses to climate can be very rapid. When extrapolated to soil temperatures encountered in nests within the introduced range, embryo retention and faster developmental rate result in one to several weeks earlier emergence compared with the ancestral state. We show that this difference translates into substantial survival benefits for offspring. This should promote short- and long-term persistence of non-native populations, and ultimately enable expansion into areas that would be unattainable with incubation duration representative of the native range.
Dostawca treści:: Repozytorium Uniwersytetu Jagiellońskiego