Autor: Richter, Lars - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: Exploring the Synergies of Single-Molecule Fluorescence and 2D Materials Coupled by DNA
Autorzy:: Kamińska, Izabela
Richter, Lars
Manzanares-Palenzuela, Lorena
Szalai, Alan M.
Tinnefeld, Philip
Wydawca:: Wiley Online Library
Cytata wydawnicza:: Richter, L., Szalai, A. M., Manzanares‐Palenzuela, C. L., Kamińska, I., & Tinnefeld, P. (2023). Exploring the Synergies of Single‐Molecule Fluorescence and 2D Materials Coupled by DNA. Advanced Materials, 35 (41), 2303152. https://doi.org/10.1002/adma.202303152
Opis:: The world of 2D materials is steadily growing, with numerous researchers attempting to discover, elucidate, and exploit their properties. Approaches relying on the detection of single fluorescent molecules offer a set of advantages, for instance, high sensitivity and specificity, that allow the drawing of conclusions with unprecedented precision. Herein, it is argued how the study of 2D materials benefits from fluorescence-based single-molecule modalities, and vice versa. A special focus is placed on DNA, serving as a versatile adaptor when anchoring single dye molecules to 2D materials. The existing literature on the fruitful combination of the two fields is reviewed, and an outlook on the additional synergies that can be created between them provided.
Dostawca treści:: Repozytorium Centrum Otwartej Nauki

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Skocz do pozycji: 2.

Tytuł:: Expanding the range of graphene energy transfer with multilayer graphene
Autorzy:: Leidinger, Paul
Kamińska, Izabela
Richter, Lars
Günther, Sebastian
Gronkiewicz, Karolina
Ploetz, Evelyn
Pyrcz, Patryk
Knechtel, Fabian
Tinnefeld, Philip
Wydawca:: Royal Society of Chemistry
Cytata wydawnicza:: Nanoscale, 2024,16, 13464-13470. https://doi.org/10.1039/D4NR01723D
Opis:: The interaction between single emitters and graphene in the context of energy transfer has attracted significant attention due to its potential applications in fields such as biophysics and super-resolution microscopy. In this study, we investigate the influence of the number of graphene layers on graphene energy transfer (GET) by placing single dye molecules at defined distances from monolayer, bilayer, and trilayer graphene substrates. We employ DNA origami nanostructures as chemical adapters to position the dye molecules precisely. Fluorescence lifetime measurements and analysis reveal an additive effect of graphene layers on the energy transfer rate extending the working range of GET up to distances of approximately 50–60 nm. Moreover, we show that switching a DNA pointer strand between two positions on a DNA origami nanostructure at a height of >28 nm above graphene is substantially better visualized with multilayer graphene substrates suggesting enhanced capabilities for applications such as biosensing and super-resolution microscopy for larger systems and distances. This study provides insights into the influence of graphene layers on energy transfer dynamics and offers new possibilities for exploiting graphene's unique properties in various nanotechnological applications.
Dostawca treści:: Repozytorium Centrum Otwartej Nauki

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Skocz do pozycji: 3.

Tytuł:: Integration of highly sensitive large-area graphene-based biosensors in an automated sensing platform
Autorzy:: Kamińska, Izabela
Meincke, Melanie
Richter, Lars
Fertig, Niels
Bazzone, Andre
Ploetz, Evelyn
George, Michael
Holzhauser, Stephan
Knechtel, Fabian
Barthmes, Maria
Tinnefeld, Philip
Wydawca:: Elsevier
Cytata wydawnicza:: Meincke, M., Bazzone, A., Holzhauser, S., Barthmes, M., Richter, L., Knechtel, F., Ploetz, E., George, M., Fertig, N., Kamińska, I., & Tinnefeld, P. (2025). Integration of Meincke, M., Bazzone, A., Holzhauser, S., Barthmes, M., Richter, L., Knechtel, F., Ploetz, E., George, M., Fertig, N., Kamińska, I., & Tinnefeld, P. (2025). Integration of highly sensitive large-area graphene-based biosensors in an automated sensing platform. In Measurement (Vol. 240, p. 115592). Elsevier BV. https://doi.org/10.1016/j.measurement.2024.115592
Opis:: Graphene-based biosensors, featuring exceptional electronic, mechanical, and surface properties, have emerged as frontrunners in advanced sensing technologies. However, to achieve widespread industrial adoption, advancements in the fabrication and integration of large-area graphene devices are essential. Critical parameters such as enhanced sensitivity, scalable production methods, economic viability, integration capabilities, and consistent uniformity must be meticulously addressed. In this work, we demonstrate that our ultra-clean, chemical wet transfer protocol of large-area graphene enables a scalable, smooth integration of graphene into an established assay platform for transporter protein drug discovery. Furthermore, we demonstrate sensitive detection of electrolytic buffers, varying pH, bovine serum albumin (BSA) and single-stranded DNA (ssDNA) adsorption, using our large-area graphene solution-gated field-effect transistor (SGFET) sensors, thereby proving their robust and reliable performance. The sensors’ biocompatibility and ion sensitivity, down to the picomolar range, substantiate their suitability for the investigation of electroactive transport in ion channels and membrane transporters
Dostawca treści:: Repozytorium Centrum Otwartej Nauki

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