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Wyświetlanie 1-10 z 21
Tytuł:
Przekaźnictwo sygnału wapniowego w zrekonstruowanych oocytach i klonalnych zarodkach ssaków w warunkach sztucznej aktywacji
Calcium signal transduction in reconstructed oocytes and somatic cell nuclear transfer embryos of mammals in the conditions of artificial activation
Autorzy:
Samiec, Marcin
Wydawca:
Committee on Biotechnology PAS
Komitet Biotechnologii PAN
Institute of Bioorganic Chemistry PAS
Instytut Chemii Bioorganicznej PAN
Powiązania:
Biotechnologia, vol.88, 1 (2010)-.
Opis:
One of the most important factors that affect the developmental competences of mammalian somatic cell nuclear transfer (SCNT) embryos is artificial activation of reconstructed oocytes (clonal cybrids). However, calcium signal transduction in a cytosol of such oocytes that has been initiated incorrectly by physical or chemical activating factors (electric pulses or specific ionophore antibiotics) can stimulate not only the development of cloned embryos. It can also induce apoptotic cell death following considerable elevation in intracellular calcium concentration and thereby excitotoxicity of Ca^+ ions. Therefore, the basic objective of this paper is to present the current knowledge on the mechanisms regulating biochemical and biophysical proapoptotic changes within SCNT embryo which were triggered by the process of excitotoxic calcium signal transmission resulting from an improper artificial activation of clonal cybrid.
prace przeglądowe
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Tytuł:
Artificial activation of porcine oocytes in somatic cell cloning procedure - biotechnological, genetic and epigenetic aspects
Biotechnologiczne, genetyczne oraz epigenetyczne aspekty sztucznej aktywacji rekonstruowanych oocytów w klonowaniu somatycznym świń
Autorzy:
Samiec, Marcin
Wydawca:
Committee on Biotechnology PAS
Komitet Biotechnologii PAN
Institute of Bioorganic Chemistry PAS
Instytut Chemii Bioorganicznej PAN
Powiązania:
2009
Biotechnologia, vol.86, 3 (2009)-.
Opis:
In the somatic cell cloning of pigs, nuclear transfer-derived oocytes are artificially stimulated with the use one of the three experimental protocols; 1) electrical, chemical or physicochemical delayed activation (i.e., post-activation); 2) simultaneous fusion and electrical activation (SF-EA) or simultaneous electrofusion and physicochemical activation, as well as 3) chemical sequential (combined) electrical and chemical activation.
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Tytuł:
Biochemiczne, molekularne i biofizyczne uwarunkowania dojrzewania i aktywacji oocytów świni w aspekcie zapłodnienia oraz klonowania somatycznego
Maturation and activation of porcine oocytes in the aspect of fertilization and somatic cell cloning - biochemical, molecular and biophysical determinants
Autorzy:
Samiec, Marcin
Wydawca:
Committee on Biotechnology PAS
Komitet Biotechnologii PAN
Institute of Bioorganic Chemistry PAS
Instytut Chemii Bioorganicznej PAN
Powiązania:
2009
Biotechnologia, vol.86, 3 (2009)-.
Opis:
The commonly used source of nuclear recipient cells in the somatic cell cloning of pigs are in wVo-matured (ovulated) or in v;tro-matured oocytes, reversibly blocked at the second metaphase (Mil) stage. One of the most important factors that significantly affect the developmental competences of porcine cloned embryos is the artificial activation of oocytes reconstructed with somatic cell nuclei.
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Tytuł:
The Bcl-2 family proteins and calcium signaling in mammalian embryos generated by somatic cell cloning
Białka z rodziny Bcl-2 a przekaźnictwo sygnału wapniowego w zarodkach ssaków uzyskiwanych techniką klonowania somatycznego
Autorzy:
Samiec, Marcin
Wydawca:
Committee on Biotechnology PAS
Komitet Biotechnologii PAN
Institute of Bioorganic Chemistry PAS
Instytut Chemii Bioorganicznej PAN
Powiązania:
Biotechnologia, vol.88, 1 (2010)-.
Opis:
The efficiency of somatic cell nuclear transfer (SCNT) technology in mammalian species remains unsatisfactory. One of the main causes of low developmental capability of pre- and peri-implanted somatic cell cloned embryos is the high occurrence of apoptotic cell death, which is prompted by incorrect calcium signaling. The latter is accompanied by upregulation of the members from the Bcl-2 protein family in the blastomeres of SCNT embryos derived from the reconstructed oocytes exposed to artificial activating factors that induce the phenomenon of Ca2+ ion excitotoxicity. Overexpression of antiapoptotic proteins from the Bcl-2 family plays a fundamental role in suppression of different pathways involving intracellular transduction of programmed cell death signal in the somatic cell cloned embryos. Enhancement of Bcl-2 synthesis in the cytoplasm as well as on the outer/cytoplasmic surface of cisterns and tubules of granular endoplasmic reticulum (ERg) and thereby increase in its concentration and activity in the membranes of ER and mitochondria prevents the redistribution of free calcium cations from ER to mitochondria. The purpose of this article is to provide an overview of the current knowledge on molecular aspects of controlling calcium intracellular homeostasis in mammalian SCNT embryos, in which apoptotic cell death was stimulated by an improper activation of reconstituted oocytes.
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Tytuł:
The use of new strategies in the studies on somatic cell cloning in pigs
Wykorzystanie nowych kierunków badań w klonowaniu somatycznym świń
Autorzy:
Skrzyszowska, Maria
Samiec, Marcin
Wydawca:
Committee on Biotechnology PAS
Komitet Biotechnologii PAN
Institute of Bioorganic Chemistry PAS
Instytut Chemii Bioorganicznej PAN
Powiązania:
Biotechnologia, vol.72, 1 (2006)-.
Opis:
Somatic cell nuclear transfer (SCNT) technique in pigs remains relatively low (2% to 5% of produced piglets), that is why further efforts have to be made to optimize both a multi-step cloning procedure and to improve a structu- ro-functiona! quality of recipient oocytes and nuclear donor cells. Pre- and postimplantation developmental potential of porcine SCNT-derived embryos depends to a high degree on not only coordination of mitotic cycle stage with phenotype of nuclear donor cell, but also proper combination of the methods of maternal chromosome elimination (enucleation), oocyte reconstruction techniques, the systems of artificial activation of generated nuclear-cytoplasmic hybrids (clonal cybrids) and in vitro culture of reconstructed embryos. Generally, it can result in increasing the competences of both somatic nuclear and mitochondrial genome for epigenetic remodeling/reprogramming in developing cloned embryos.
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Tytuł:
Rozwój badań nad klonowaniem somatycznym kóz
Development of the studies on somatic cell cloning in goats
Autorzy:
Skrzyszowska, Maria
Samiec, Marcin
Wydawca:
Committee on Biotechnology PAS
Komitet Biotechnologii PAN
Institute of Bioorganic Chemistry PAS
Instytut Chemii Bioorganicznej PAN
Powiązania:
Biotechnologia, vol.72, 1 (2006)-.
Opis:
Domestic goat as a species with a relatively great biodiversity of dairy breeds, which possess high genetic merit and yield of milk production, can be a valuable tool for embryo gene engineering. This involves the generation of transgenic specients, providing with xenogeneic (human) recombinant proteins (i.e. biopharmaceuticals), not only by the standard zygote intrapronuclear microinjection of gene constructs, but above all with the use of somatic cell cloning technology.
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Tytuł:
The role of pig in somatic cell cloning and transgenesis
Znaczenie świni dla klonowania somatycznego i transgenezy
Autorzy:
Samiec, Marcin
Skrzyszowska, Maria
Wydawca:
Committee on Biotechnology PAS
Komitet Biotechnologii PAN
Institute of Bioorganic Chemistry PAS
Instytut Chemii Bioorganicznej PAN
Powiązania:
Biotechnologia, vol.72, 1 (2006)-.
Opis:
A stimulus for development of the studies on pig somatic cell cloning, especially in recent years, was above all the possibility of its practical application for production of transgenic piglets using in vitro transfected nuclear donor cells and multiplication of genetically-engineered sows and boars generated so far, on the grounds of important implications for biomedicine, pharmacy and agriculture. However, effective pig somatic cell nuclear transfer, avoiding the sexual reproduction pathway, creates a possibility of providing numerous monoge- netic and monosexual offspring derived not only from genetically-transformed individuals, but also from adult (postpubertal) animals of high genetic merit. Generation of cloned transgenic pigs for biomedical purposes to obtain recombinant xenogeneic proteins or organs suitable in xenotransplantology, or to create cell (gene) therapy foundations for a number of serious monogenic diseases that induce heritable (congenital) developmental anomalies, is perceived as a service to humanity.
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Autorzy:
Duda, Małgorzata
Samiec, Marcin
Opis:
Granulosa cells (GCs) and ovarian putative stem cells (oPSCs) represent distinct but complementary populations within the mammalian ovary. While GCs have long been considered terminally differentiated and hormonally specialized, emerging evidence indicates that they retain epigenetic plasticity and, under defined conditions, can be reprogrammed into cells exhibiting pluripotent-like features. In contrast, oPSCs, including oogonial stem cells (OSCs) and very small embryonic-like stem cells (VSELs), are naturally multipotent and capable of spontaneous or inducible differentiation into neural, endothelial, and other somatic lineages. Both cell types express stemness-related markers, such as OCT4, SOX2, and c-KIT, and demonstrate potential for self-renewal and lineage conversion. Recent advances in chemical modulation of epigenetic reprogramming, particularly with agents from the family of non-specific DNA methyltransferase (DNMT) inhibitors, such as 5-azacytidine (5-azaC), highlight the feasibility of generating functional, lineage-specific derivatives of GCs or oPSCs without genetic manipulation. Not without significance is also the fact that extended/high-dose 5-azaC-mediated modulation can induce cell senescence or apoptotic/necrotic death. Therefore, dosing must be carefully titrated, which strongly supports a dose- and/or time-dependent mechanism for 5-azaC-based epigenetic modification in treated cells. This study aims to summarize the molecular and functional properties of mammalian GCs and oPSCs, emphasizing their applicability in regenerative medicine and reproductive bioengineering, with a focus on safe, patient-specific cell-based therapies.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Wyświetlanie 1-10 z 21

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