- Tytuł:
- Functional reconstitution of Ral-binding GTPase activating protein, RLIP76, in proteoliposomes catalyzing ATP-dependent transport of glutathione conjugate of 4-hydroxynonenal.
- Autorzy:
-
Sharma, Rajendra
Sharma, Abha
Yang, Yusong
Awasthi, Sanjay
Singhal, Sharad
Zimniak, Piotr
Awasthi, Yogesh - Tematy:
-
RLIP76
4-hydroxynonenal
Ral-binding GTPase activating protein
transport
glutathione conjugate of 4-hydroxynonenal - Pokaż więcej
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Powiązania:
- https://bibliotekanauki.pl/articles/1043735.pdf Link otwiera się w nowym oknie
- Opis:
- Earlier studies from our laboratories have shown that RLIP76, a previously described Ral-binding GTPase activating protein (Jullien-Flores et al., 1995, J. Biol. Chem. 270: 22473), is identical with the xenobiotic transporter DNP-SG ATPase, and can catalyze ATP-dependent transport of glutathione-conjugates as well as doxorubin (Awasthi et al., 2000, Biochemistry, 39: 9327). We have now reconstituted purified bacterially expressed RLIP76 in proteoliposomes, and have studied ATP-dependent uptake of the glutathione conjugate of 4-hydroxynonenal (GS-HNE) by these vesicles. Results of these studies show that RLIP76 reconstituted in proteoliposomes catalyzes ATP-dependent transport of GS-HNE against a concentration gradient. The transport of GS-HNE is saturable with respect to ATP as well as GS-HNE with Km values of 1.4 mM and 2.5 μM, respectively. These studies demonstrate that RLIP76 mediates active transport of GS-HNE, and are consistent with our previous work showing that RLIP76-mediated efflux of GS-HNE regulates the intracellular concentration of 4-HNE and thereby affects 4-HNE mediated signaling.
- Dostawca treści:
- Biblioteka Nauki
Artykuł