Autor: Tian, Lin - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: Sp100 interacts with phage ΦC31 integrase to inhibit its recombination activity
Autorzy:: Lin, Yun
Li, Zhi-Hui
Wang, Jing-Jing
Xu, Gua-Lan
Shen, Qi
Tian, Lin
Xue, Jin-Lun
Chen, Jin-Zhong
Tematy:: Sp100
ΦC31 integrase
recombination; Pokaż więcej
Wydawca:: Polskie Towarzystwo Biochemiczne
Powiązania:: https://bibliotekanauki.pl/articles/1039951.pdf Link otwiera się w nowym oknie
Opis:: Phage ΦC31 integrase is a potential vector for the insertion of therapeutic genes into specific sites in the human genome. To understand the mechanism involved in ΦC31 integrase-mediated recombination, it is important to understand the interaction between the integrase and cellular proteins. Using a yeast two-hybrid system with pLexA-ΦC31 integrase as bait, we screened a pB42AD human fetal brain cDNA library for potential interacting cellular proteins. From the 106 independent clones that were screened, 11 potential interacting clones were isolated, of which one encoded C-terminal fragment of Sp100. The interaction between Sp100 and ΦC31 integrase was further confirmed by yeast mating and co-immunoprecipitation assays. The hybridization between a ΦC31 integrase peptide array and an HEK293 cell extract revealed that residues 81RILN84 in the N-terminus of ΦC31 integrase are responsible for the interaction with Sp100. Knocking down endogenous Sp100 with Sp100-specific siRNA increased ΦC31 integrase-mediated recombination but did not impact reporter gene expression. Therefore, endogenous Sp100 may interact with ΦC31 integrase and inhibit the efficiency of ΦC31 integrase-mediated recombination.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Multiphase extraction of ephedrine from Pinellia ternata using bionic liquid-modified polymer
Autorzy:: Fang, Luwei
Tian, Minglei
Lin, Liangwen
Row, Kyung Ho
Tematy:: multiphase extraction
bionic liquid
polymer
adsorption
ephedrine
Pinellia ternata; Pokaż więcej
Wydawca:: Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Powiązania:: https://bibliotekanauki.pl/articles/779059.pdf Link otwiera się w nowym oknie
Opis:: Multiphase extraction (MPE) was applied as a developed, convenient and efficient method in separation of ephedrine from Pinellia ternata. Firstly, in order to increase the adsorption efficiency, bionic liquid-modified polymer was created. Comparing the effects of all sorbents under variables conditions, the highest amount of 5.8 mg/g can be adsorbed on dual imidazole ionic liquid modified polymer (Im-Im-Poly) in methanol/water (70:30, v/v) solution at 25°C within 30.0 min. Then the Im-Im-Poly was applied in MPE, after 7 times repetition of extraction, around 1.0 mg/g of ephedrine from Pinellia ternata was detected. After washing by water, ethanol and methanol, and elution by methanol/acetic acid (99.0:1.0, v/v), ephedrine was successfully separated.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Adsorption efficiency of pentafluorobenzene on ionic liquids-based silicas
Autorzy:: Wang, X.
Lin, L.
Xie, J.
Yan, X.
Xiao, W.
Tian, P.
Tematy:: ionic liquids based silica
pentafl uorobenzene
adsorption
solid-phase extraction; Pokaż więcej
Wydawca:: Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Powiązania:: https://bibliotekanauki.pl/articles/778559.pdf Link otwiera się w nowym oknie
Opis:: The adsorption of pentafl uorobenzene on nine ionic liquid-based silicas was investigated using solid phase extraction. The effects of several variables such as the type of ionic liquid groups, adsorption time, temperatures and water ratio in the solution system were experimentally evaluated. The imidazole-chloride ionic liquid group based silica exhibited the highest adsorption efficiency under the optimized conditions of 5 min adsorption at 30°C in water/methanol (30:70, vol%) solution. In addition, the effects of pH, as well as type and concentrations of chloride salts were investigated. At pH values other than neutral and high salt concentration, the adsorption efficiency was reduced. Finally, the relative standard deviation of less than 5.8% over a 5-day period showed a high precision for the nine tested sorbents.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Stirred-tank leaching of coarse-grained waste, printed circuit boards with Acidithiobacillus ferrooxidans
Autorzy:: Fu, Kaibin
Tian, Li
Hou, Puyao
Long, Meiqiao
Chen, Shu
Lin, Hai
Tematy:: stirred tank leaching
coarse-grained WPCBs
A ferrooxidans
H+ consumption
Avrami equation; Pokaż więcej
Wydawca:: Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Powiązania:: https://bibliotekanauki.pl/articles/2146844.pdf Link otwiera się w nowym oknie
Opis:: Stirred tank leaching of metals from coarse-grained waste, printed circuit boards (WPCB) used Acidithiobacillus ferrooxidans (A. ferrooxidans) at ambient temperature (20-35°C). The effect of the baffle size, WPCB concentration, and inoculation volume was tested. 95.92% of Cu, 93.53% of Al, 92.58% of Zn, 65.27% of Ni, and 95.33% of Sn in WPCBs were leached under the optimal conditions: no baffle, WPCB concentration of 5.0% (w/w), and inoculation volume of 5% (v/v). The alkaline substance and reactivity metal of WPCBs, and the oxidation of Fe2+, consume H+. Adding acid can maintain the pH value of the leaching solution, which is conducive to the growth and reproduction of the bacteria and improves the leaching efficiency of WPCBs. The second-order dynamics model can describe the acid consumption in the bioleaching process of coarse-grained WPCBs. Moreover, the Avrami equation can successfully explain the bioleaching kinetics of Cu, Al, Zn, Ni, and Sn from the coarse-grained WPCBs. The key factors controlling the bioleaching of coarse-grained WPCBs are metal reactivity and specific surface area. These results revealed that bioleaching metals from coarse-grained WPCBs using A.ferrooxidans is feasible, and has important significance to guiding its industrialization.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Lipopolysaccharide accelerates fine particulate matter-induced cell apoptosis in human lung bronchial epithelial cells
Autorzy:: Ru, Qin
Xiong, Qi
Chen, Lin
Tian, Xiang
Yue, Kai
Ma, Baomiao
Liu, Lu
Wu, Rihui
Xu, Congyue
Pi, Mingshan
Li, Chaoying
Tematy:: Apoptosis
cytotoxicity
inflammation
fine particulate matter
free radicals
bronchial epithelial cells; Pokaż więcej
Wydawca:: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Powiązania:: https://bibliotekanauki.pl/articles/2161782.pdf Link otwiera się w nowym oknie
Opis:: Objectives The aim of the study has been to investigate the effect of the Standard Reference Material of fine particulate matter (SRM 2786) on cytotoxicity and apoptosis in human lung bronchial epithelial cells (16HBE cells). Whether the lipopolysaccharide (LPS)-induced inflammation could further accelerate cell apoptosis induced by SRM 2786 stimulation has also been determined. Material and Methods 16HBE cells were exposed to various doses of SRM 2786 with or without LPS. The following parameters: cytotoxicity, apoptotic rate, Bax/Bcl-2 expression, nitric oxide (NO) production, and reactive oxygen species (ROS) generation were measured. Results The results have shown that SRM 2786 induces cell damage and apoptosis of 16HBE cells as demonstrated by significant decrease in expression of Bcl-2 and increase in expression of Bax. When compared with the control cells, the apoptotic rate of cells treated by 500 μg/ml of SRM 2786 increased from 2.43±0.21% to 43.96±2.95% (p < 0.01). Further, there was an elevated production of NO and ROS post SRM 2786 treatment. The level of NO in cells treated with 500 μg/ml of SRM 2786 was 18.33±1.02 μmol/l whereas that of control cells was 1.58±0.31 μmol/l (p < 0.01). When compared with the control group, the level of intracellular ROS increased by 24% after treatment with 500 μg/ml of SRM 2786 (p < 0.05). In addition, LPS pre-treatment may accelerate cell apoptosis by increasing generation of NO and ROS followed by SRM 2786 stimulation. When compared to cells treated with 125 μg/ml of SRM 2786 alone, the levels of NO and ROS in cells pretreated with LPS increased by 28% and 11.6%, respectively (p < 0.05), and the apoptotic rate increased from 34.62±4.44% to 54.11±3.34% (p < 0.01). Conclusions These findings have suggested that in vitro exposure to SRM 2786 could induce 16HBE cells apoptosis probably by means of the mechanism involving the generation of free radicals, while the degree of apoptosis would be further aggravated under inflammation condition. Int J Occup Med Environ Health 2018;31(2):173–183
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Intervention effects of greenspace exposure on human microbiota : a randomized controlled trial in Chinese young adults
Autorzy:: Dong, Guang-Hui
Huang, Ji-Lin
Markevych, Iana
Dadvand, Payam
Yang, Bo-Yi
Heinrich, Joachim
Li, Jun-Yi
Chen, Chen
Browing, Matthew H.E.M.
Li, Chuang
Zheng, Zi-Han
Jiang, Jian-Cheng
Zhao, Tian-Yu
Zhu, Xiao-Qi
Wang, Zhi
Wang, Lu
Opis:: Enriching human microbiota has been proposed as a mechanism by which greenspace exposure improves human health. The existing evidence is scarce with few studies able to evaluate causality. We conducted a randomized controlled trial of 30 healthy undergraduate students to explore the intervention effects of greenspace on human gut and oral microbiota alpha-diversity, composition, differential genera and functional pathways. The study participants were divided into three groups, including outdoor greenspace (GS) group, outdoor non-greenspace (NGS) group, and indoor group, who visited a park, an open space without vegetation, and a classroom, respectively, for two hours per day over seven days. Differences in microbial alpha-diversity and composition across various groups were tested using Wilcoxon test and permutational multivariate analysis of variance, respectively. Linear discriminant analysis effect size analysis was performed to test differences in genera and functional pathways. Greenspace intervention significantly increased gut microbiota alpha-diversity, especially the observed Amplicon Sequence variant indexes and the Faith indexes (both p <0.05). In addition, the intervention substantially changed the composition of gut microbiota, of which the relative abundances of potentially beneficial bacteria increased. Further, the greenspace intervention affected several functional pathways of gut microbiota, including "substance dependence", "specific types of cancer", and "viral infectious diseases". However, we did not find any significant effect of greenspace intervention on oral microbiota. Our results suggest that greenspace intervention diversifies the gut microbiota and alters its composition. These findings could help to reinforce the potential of increasing people’s access to greenspace as a public health intervention.
Dostawca treści:: Repozytorium Uniwersytetu Jagiellońskiego