- Tytuł:
- Analysis of metagenomic data
- Autorzy:
-
Francis, Dallace
Muralidharan, Harihara Subrahmaniam
Sharma, Nitesh Kumar
Pop, Mihai
Koslicki, David
Chikhi, Rayan
Zelikovsky, Alex
Błaszczyk, Dagmara
Ganda, Erika
Ronkowski, Cynthia
Kościółek, Tomasz
Rodriguez, Judith S.
Gancz, Abigail Shahar
Crandall, Keith A.
Ghiasi, Nika Mansouri
Łabaj, Paweł
Tierney, Braden T.
Van Syoc, Emily
Munteanu, Viorel
Sun, Fengzhu
Vicedomini, Riccardo
Alser, Mohammed
Mangul, Serghei
Luan, Tu
Rahnavard, Ali
Frolova, Alina
Jaiswal, Pooja
Mason, Christopher
Moustafa, Ahmed M.
Liu, Shaopeng
Andreace, Francesco
Blekhman, Ran
Zielińska, Kinga
Tian, Shuchang
Mutlu, Onur
Della Libera, Katja
Łabaj, Wojciech
Zackular, Joseph P.
Davenport, Emily R.
Huntley, Naomi E. - Opis:
- Metagenomics has revolutionized our understanding of microbial communities, offering unprecedented insights into their genetic and functional diversity across Earth’s diverse ecosystems. Beyond their roles as environmental constituents, microbiomes act as symbionts, profoundly influencing the health and function of their host organisms. Given the inherent complexity of these communities and the diverse environments where they reside, the components of a metagenomics study must be carefully tailored to yield accurate results that are representative of the populations of interest. This Primer examines the methodological advancements and current practices that have shaped the field, from initial stages of sample collection and DNA extraction to the advanced bioinformatics tools employed for data analysis, with a particular focus on the profound impact of next-generation sequencing on the scale and accuracy of metagenomics studies. We critically assess the challenges and limitations inherent in metagenomics experimentation, available technologies and computational analysis methods. Beyond technical methodologies, we explore the application of metagenomics across various domains, including human health, agriculture and environmental monitoring. Looking ahead, we advocate for the development of more robust computational frameworks and enhanced interdisciplinary collaborations. This Primer serves as a comprehensive guide for advancing the precision and applicability of metagenomic studies, positioning them to address the complexities of microbial ecology and their broader implications for human health and environmental sustainability.
- Dostawca treści:
- Repozytorium Uniwersytetu Jagiellońskiego
Artykuł