Temat: Colpoda - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: Evaluation of Intracellular Ca2+ Concentration by Fura 2 Ratiometry in Encystment-induced Colpoda cucullus
Autorzy:: Matsuoka, Tatsuomi
Tematy:: Colpoda, Ca2+, encystment induction, fura 2; Pokaż więcej
Wydawca:: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Powiązania:: https://bibliotekanauki.pl/articles/763521.pdf Link otwiera się w nowym oknie
Opis:: In Colpoda cucullus, the signaling pathways for encystment induction involving protein phosphorylation have been believed to be triggered by an increase in the intracellular Ca2+ concentration promoted by cell-to-cell mechanical contact due to overpopulation. By means of fura 2 ratiometry, the present study showed that the intracellular Ca2+ concentration was actually elevated when vegetative cells were induced to encyst by being suspended at a high cell density in the presence of external free Ca2+ and suppressed by chelating external Ca2+. This result strongly suggests that an increase in the intracellular Ca2+ concentration caused by an inflow of Ca2+ promoted by cell-tocell mechanical contact due to overpopulation enhances the rate of encystation in Colpoda cucullus.
Dostawca treści:: Biblioteka Nauki

Artykuł

Skocz do pozycji: 2.

Tytuł:: Gamma Radiation Tolerance and Protein Carbonylation Caused by Irradiation of Resting Cysts in the Free-living Ciliated Protist Colpoda cucullus
Autorzy:: Saito, Ryota
Koizumi, Ryota
Sakai, Tatsuya
Shimizu, Taiga
Ono, Taiki
Sogame, Yoichiro
Tematy:: Colpoda
cyst
gamma radiation
cell repair
protein carbonylation; Pokaż więcej
Wydawca:: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Powiązania:: https://bibliotekanauki.pl/articles/52060279.pdf Link otwiera się w nowym oknie
Opis:: The ciliate Colpoda cucullus forms resting cysts to survive unfavorable environmental stresses. In this study, we have shown that Colpoda resting cysts survived exposure to a gamma radiation dose of 4000 Gy, although vegetative cells were killed by 500 Gy. After 4000 Gy irradiation, more than 90% of resting cysts and approximately 70% of dry cysts could excyst to form vegetative cells. In both cases, the excystment gradually increased after the induction of excystment. In addition, we also showed that protein carbonylation level was increased by gamma irradiation, but decreased by incubation in the cyst state. These results indicated that cell damage was repaired in resting cysts. Colpoda probably developed tolerance to gamma radiation by forming resting cysts as a strategy for growth in terrestrial environments, as part of contending with the stress due to reactive oxygen species caused by desiccation.
Dostawca treści:: Biblioteka Nauki

Artykuł

Skocz do pozycji: 3.

Tytuł:: Evidence of Stress Recovery in Free-Living Ciliate Colpoda cucullus: The Repair Capability of Resting Cysts to Damage Caused by Gamma Irradiation
Autorzy:: Sogame, Yoichiro
Saito, Ryota
Koizumi, Ryota
Shimizu, Taiga
Ono, Taiki
Tematy:: Stress recovery
Colpoda
Cryptobiosis
Resting cyst
Gamma irradiation; Pokaż więcej
Wydawca:: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Powiązania:: https://bibliotekanauki.pl/articles/52073946.pdf Link otwiera się w nowym oknie
Opis:: In this study, we report that the unicellular free-living protist Colpoda cucullus in the resting cyst (cryptobiosis) repairs stress damage. We previously demonstrated that resting cysts of Colpoda cucullus have extreme tolerance to gamma irradiation and can revert to vegetative cells after irradiation. Such irradiated cysts gradually excyst, suggesting that stress repair mechanisms are active during excystment or in the resting cyst. Herein we provide bioassay evidence that the rate of excystment of irradiated cysts is elevated by subsequent incubation, thereby indicating that cells injured by gamma irradiation can repair themselves in the resting cyst, whereas irradiated dry cysts cannot.
Dostawca treści:: Biblioteka Nauki

Artykuł

Skocz do pozycji: 4.

Tytuł:: Photoresponse in the Ciliated Protozoan Colpoda cucullus
Autorzy:: Kawano, Noriyuki
Funadani, Ryoji
Arikawa, Mikihiko
Harada, Tetsuo
Suizu, Futoshi
Matsuoka, Kou
Matsuoka, Tatsuomi
Tematy:: Colpoda
resting cyst
photodispersal
photokinetic response
action spectrum; Pokaż więcej
Wydawca:: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Powiązania:: https://bibliotekanauki.pl/articles/52376307.pdf Link otwiera się w nowym oknie
Opis:: We found that vegetative cells of Colpoda cucullus Nag-1 accumulated in shaded areas of a container when grown in the laboratory and then formed resting cysts. The photodispersal (negative photoaccumulation) of C. cucullus was mediated, at least in part, by a difference in forward swimming velocity between the illuminated region and the shaded area of the Petri dish (motion slowed or stopped in the shaded area). When C. cucullus was stimulated by continuous light irradiation, the forward swimming velocity increased and reached a steady state within 10 s. When the light intensity decreased, the forward swimming velocity gradually decreased, and eventually returned to its original level for approximately 1 min. The action spectrum of the photokinetic response (steady-state swimming acceleration driven by continuous light stimulation) implies the involvement of blue light receptors.
Dostawca treści:: Biblioteka Nauki

Artykuł

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