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Wyświetlanie 1-10 z 27
Tytuł:
Dicationic derivatives of dinaphthotetraaza[14]annulene : synthesis, crystal structures and the preliminary evaluation of their DNA binding properties
Autorzy:
Nowak, Justyna
Piantanida, Ivo
Stadnicka, Katarzyna
Kaźmierska, Alicja
Sieroń, Lesław
Eilmes, Andrzej
Eilmes, Julita
Gryl, Marlena
Matković, Marija
Radić Stojković, Marijana
Opis:
Four new water-soluble, fluorescent derivatives of dinaphthotetraaza[14]annulene (DNTAA) have been synthesized varying in the structure, dimensions and spatial arrangements of their meso side groups. The products have been carefully characterized by elemental analyses, spectroscopy, crystal structures and quantum-chemical calculations employing DFT methodology. One representative product of the DNTAA series was tested for DNA binding using UV–vis titrations, CD and thermal denaturation experiments. Intercalation of ds-DNA was recognized as a dominant binding mode. In contrast to non-fluorescent phenylene analogues (DBTAA), reported previously, DNTAA derivative showed threefold fluorescence increase upon DNA binding, and offered intriguing new applications as a fluorescent DNA/RNA dye.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Tytuł:
Characterization of a novel protein that specifically binds to DNA modified by N-acetoxy-acetylaminofluorene and cis-diamminedichloroplatinum
Autorzy:
Pietrowska, Monika
Widłak, Piotr
Tematy:
damage recognition
cisplatin
damaged DNA-binding protein
acetylaminofluorene
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Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1041333.pdf  Link otwiera się w nowym oknie
Opis:
Proteins recognizing DNA damaged by the chemical carcinogen N-acetoxy-acetylaminofluorene (AAAF) were analyzed in nuclear extracts from rat tissues, using a 36 bp oligonucleotide as a substrate and electrophoretic mobility shift and Southwestern blot assays. One major damage-recognizing protein was detected, whose amount was estimated as at least 105 copies per cell. Levels of this protein were similar in extracts from brain, kidney and liver, but much lower in extracts from testis. The affinity of the detected protein for DNA damaged by AAAF was about 70-fold higher than for undamaged DNA. DNA damaged by cis-diamminedichloroplatinum (cis-DDP), benzo(a)pyrene diolepoxide (BPDE) or UV-radiation also bound this protein with an increased affinity, the former more strongly and the latter two more weakly as compared to AAAF-damaged DNA. The detected AAAF/DDP-damaged-DNA-binding (AAAF/DDP-DDB) protein had a molecular mass of about 25 kDa and was distinct from histone H1 or HMGB proteins, which are known to have a high affinity for cis-DDP-damaged DNA. The level of this damage-recognizing protein was not affected in rats treated with the carcinogen 2-acetylaminofluorene. The activity of an AAAF/DDP-DDB protein could also be detected in extracts from mouse liver cells but not from the Hep2G human hepatocellular carcinoma.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro DNA binding of purified CcpA protein from Lactococcus lactis IL1403
Autorzy:
Kowalczyk, Magdalena
Borcz, Barbara
Płochocka, Danuta
Bardowski, Jacek
Tematy:
EMSA
DNA binding
carbon catabolite repression
CcpA
competition EMSA
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Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1041113.pdf  Link otwiera się w nowym oknie
Opis:
During this study His-tagged CcpA protein purified under native conditions to obtain a biologically active protein was used for molecular analysis of CcpA-dependent regulation. Using electrophoretic mobility shift assays it was demonstrated that CcpA of L. lactis can bind DNA in the absence of the HPr-Ser-P corepressor and exhibits DNA-binding affinity for nucleotide sequences lacking cre sites. However, purified HPr-Ser-P protein from Bacillus subtilis was shown to slightly increase the DNA-binding capacity of the CcpA protein. It was also observed that CcpA bound to the cre box forms an apparently more stable complex than that resulting from unspecific binding. Competition gel retardation assay performed on DNA sequences from two PEP:PTS regions demonstrated that the ybhE, bglS, rheB, yebE, ptcB and yecA genes situated in these regions are most probably directly regulated by CcpA.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of damage-recognition proteins from human lymphocytes.
Autorzy:
Łanuszewska, Joanna
Cudak, Anna
Rzeszowska-Wolny, Joanna
Widłak, Piotr
Tematy:
damage recognition
human lymphocytes
damaged DNA-binding proteins
repair capacity
Pokaż więcej
Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1044373.pdf  Link otwiera się w nowym oknie
Opis:
Proteins recognizing and binding to damaged DNA (DDB-proteins) were analyzed in human lymphocytes obtained from healthy donors. Using an electrophoretic mobility shift assay several complexes between nuclear extract proteins and damaged DNA were detected: a complex specific for DNA damaged by N-acetoxy-N-acetylaminofluorene, another complex specific for UV-irradiated DNA, and two complexes specific for DNA damaged by cis-dichlorodiammine platinum. All the detected complexes differed in electrophoretic mobility and possibly contained different proteins. Complexes specific for free DNA ends were also detected in protein extracts from lymphocytes.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vanadium rutin complex sensitizes breast cancer cells via modulation of p53/Bax/Bcl2/VEGF correlated with apoptotic events
Autorzy:
Haifeng, Zhu
Yinghou, Wang
Dan, Liu
Xiuqing, Sun
Furong, Wang
Tematy:
apoptosis
Breast Cancer
In vitro assessment
DNA Binding
Vanadium-rutin
Pokaż więcej
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Powiązania:
https://bibliotekanauki.pl/articles/895635.pdf  Link otwiera się w nowym oknie
Opis:
In pursuit of a novel approach in breast cancer therapy, we explored the ability of vanadium rutin complex to eradicate cancer by efficiently targeting various apoptotic pathways on human breast cancer cell lines. We provide direct proof of the chemotherapeutic potential of vanadium rutin complex by activating p-53 dependent intrinsic apoptosis and modulating the VEGF pathways. The complex was also capable of binding and cleaving CT-DNA at different concentration. The complex was able to inhibit cell viability at 100 and 150 µM doses in both MCF7 and MDA-MB-231 cells. Furthermore, the complex successfully initiated apoptosis in both cell lines by activating the p53 dependant intrinsic apoptotic pathway. In-vitro studies also established that the complex modulated p53, Bax, Bcl2 and VEGF expressions and induced DNA fragmentation in both the cell lines.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Iron(II) complexes containing the 2,6-bis-iminopyridyl moiety : synthesis, characterization, reactivity, and DNA binding
Autorzy:
El-Shafai, Nagi
van Eldik, Rudi
Shaban, Shaban Y.
Mansour, Hanaa
Opis:
Two iron(II) complexes, [FeII(pytBuN3)2](FeCl4) (1) and [FeII(pytBuMe2N3)Cl2] (2), with sterically constrained pytBuN3 and pytBuMe2N3 chelate ligands (pytBuN3 = 2,6-bis-(aldiimino)pyridyl; pytBuMe2N3 = 2,6-bis-(ketimino)pyridyl), have been synthesized and characterized by elemental analysis, IR, UV–vis spectra, and preliminary X-ray single-crystal diffraction. The latter revealed that Fe(II) in 1 is six-coordinate by six nitrogen donors from two bisiminopyridines in a distorted octahedron. Complex 2 reacts with thiourea with a second-order rate constant k2 = (2.50 ± 0.05) × 10−3 M−1 s−1 at 296 K, and the reaction seemed to be slow. In a similar way, the interaction of 2 and DNA was studied by fluorescence and absorption spectroscopy. The results revealed that 2 caused fluorescence quenching of DNA through a dynamic quenching procedure. The binding constants KA, Kapp, and KSV as well as the number of binding sites between 2 and DNA were determined.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Wyświetlanie 1-10 z 27

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