Temat: ISSR - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: Evaluating genetic diversity of chilling stress in cotton genotypes
Autorzy:: Sofalian, Omid
Azimy, Somayyeh
Jahanbakhsh, Sodabeh
Khomari, Saeid
Dezhsetan, Sara
Tematy:: chilling
cotton
ISSR marker; Pokaż więcej
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Powiązania:: https://bibliotekanauki.pl/articles/2199736.pdf Link otwiera się w nowym oknie
Opis:: In order to study genetic diversity and some physiological features related to chilling stress using molecular markers, an experiment was conducted at University of Mohaghegh Ardabili. Treatments were set in a factorial experiment based on randomized complete block design with 3 replications and 3 stress levels (25, 15 and 5°C) between 20 cotton genotypes. The results showed that chilling stress influenced on some physiological features such as the activity of catalase, proline content, soluble carbohydrates and proteins. Cluster analysis carried out using WARD method in physiological features showed that genotypes located in three groups in the acclimation level and after acclimation, respectively. Nazilli, Ciakra, Avangard and B 557 were in the better group in studied levels. Also based on the results Avangard, Chegurava, Tashkand and Shirpan 603 were the most tolerant genotypes. In the ISSR marker analysis using of 12 primers produced 96 polymorphic bands. The mean of PIC, MI and EMR were 0.283, 1.065 and 3 respectively, for all primers. Some of markers had promising results that confirmed ISSR markers as powerful tool in any marker assisted program for plant breeders.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Evaluating genetic diversity of chilling stress in cotton genotypes
Autorzy:: Sofalian, Omid
Azimy, Somayyeh
Jahanbakhsh, Sodabeh
Khomari, Saeid
Dezhsetan, Sara
Tematy:: chilling
cotton
ISSR marker; Pokaż więcej
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Powiązania:: https://bibliotekanauki.pl/articles/55928275.pdf Link otwiera się w nowym oknie
Opis:: In order to study genetic diversity and some physiological features related to chilling stress using molecular markers, an experiment was conducted at University of Mohaghegh Ardabili. Treatments were set in a factorial experiment based on randomized complete block design with 3 replications and 3 stress levels (25, 15 and 5°C) between 20 cotton genotypes. The results showed that chilling stress influenced on some physiological features such as the activity of catalase, proline content, soluble carbohydrates and proteins. Cluster analysis carried out using WARD method in physiological features showed that genotypes located in three groups in the acclimation level and after acclimation, respectively. Nazilli, Ciakra, Avangard and B 557 were in the better group in studied levels. Also based on the results Avangard, Chegurava, Tashkand and Shirpan 603 were the most tolerant genotypes. In the ISSR marker analysis using of 12 primers produced 96 polymorphic bands. The mean of PIC, MI and EMR were 0.283, 1.065 and 3 respectively, for all primers. Some of markers had promising results that confirmed ISSR markers as powerful tool in any marker assisted program for plant breeders.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Ocena stabilności genetycznej dwóch cytotypów Pelargonium zonale var. „Kleiner Liebling” w warunkach kultur tkankowych
Estimation of genetic stability of two cytotypes of Pelargonium zonale var. „Kleiner Liebling” in tissue culture conditions
Autorzy:: Cienki, Julia
Opis:: Pelargonium zonale var. „Kleiner Liebling” jest monoploidem, dzięki czemu stanowi ciekawy obiekt badań. W naturze występuje wiele przykładów roślin z pojedynczym zestawem chromosomów, jednak P. zonale var. „Kleiner Liebling” jako jedyna z odmian botanicznych odnosi sukcesy jako roślina ozdobna. Odmiana ta charakteryzuje się małymi liśćmi oraz kwiatami, które są liczniejsze w porównaniu do innych odmian P. zonale, co wynika z jej haploidalności. Rozmnażanie odbywa się przez sadzonki pędowe, a wegetacja jest niezależna od rozmnażania płciowego, co umożliwiło utrzymanie takiej mutacji. Przy pomocy kolchicyny, w latach 60. XX wieku, udało się podwoić liczbę chromosomów tej odmiany otrzymując formę diploidalną (podwojonego haploida). Celem niniejszej pracy była ocena stabilności genetycznej obydwu cytotypów (haploidalnego i diploidalnego) roślin macierzystych oraz regenerantów otrzymanych z wykorzystaniem kultur tkankowych należących do P. zonale var. „Kleiner Liebling”. Zbadano 10 roślin w tym pięć regenerantów powstałych w wyniku przybyszowej morfogenezy pośredniej (via klaus) i oceniono ich zmienność za pomocą dwóch metod – RAPD oraz ISSR. Dokonano również porównania tych metod. Utworzono drzewa i sieci filogenetyczne oraz wykonano analizy statystyczne. W celu otrzymania pełniejszego obrazu różnic genetycznych pomiędzy badanymi próbkami P. zonale var. „Kleiner Liebling” zbadano również inne gatunki należące do rodzaju Pelargonium oraz porównano ich zróżnicowanie genetyczne między sobą. Badania wykazały, że w badanych próbkach P. zonale var. „Kleiner Liebling” występuje pewne zróżnicowanie genetyczne pomimo ich wegetatywnego rozmnażania. Udział procentowy prążków monomorficznych dla obydwu metod wynosił jedynie 61%, jednak na podstawie drzew oraz sieci filogenetycznych próbki te zostały połączone w jeden klad, a poszczególne cytotypy nie grupowały się na osobnych gałęziach. Regeneranty wykazywały stosunkowo niewielką zmienność, która mogła być wynikiem niepełnej powtarzalności zastosowanych metod. Nie zaobserwowano znaczących różnic w stabilności genetycznej pomiędzy regenerantami obydwu cytotypów, które mogłyby wynikać z różnic w ploidalności oraz różnicy w sposobie prowadzenia hodowli in vitro obydwu cytotypów. Pozostałe badane gatunki należące do Pelargonium charakteryzowały się dużym dystansem genetycznym i nie ustalono dokładnych relacji filogenetycznych pomiędzy nimi, co mogło wynikać ze zbyt małej ilości danych. Metoda ISSR wykazała nieco mniejszy polimorfizm badanych próbek, jednak metoda RAPD charakteryzowała się dłuższymi produktami reakcji i większą liczbą prążków. Metody użyte w badaniach nie wykazywały znacznych różnic w powtarzalności wyników.
Pelargonium zonale var. 'Kleiner Liebling' is a monoploid, which makes it an interesting object of study. In nature there are many examples of plants with a single set of chromosomes, but P. zonale var. 'Kleiner Liebling' is the only one of the botanical varieties that is successful as an ornamental plant. This variety is characterized by small leaves and flowers, which are more numerous compared to other varieties of P. zonale. It is the consequence of its haploidy. Propagation is by shoot cuttings, and vegetation is independent of sexual reproduction, which made it possible to maintain such a mutation. By the use of colchicine, in the 1960s, it was possible to double the number of chromosomes of this variety and obtain a diploid form (doubled haploid). The aim of the present study was to assess the genetic stability of both cytotypes (haploid and diploid) of mother plants and regenerants obtained using tissue cultures belonging to P. zonale var. 'Kleiner Liebling'. Ten plants, including five regenerants obtained by adventitious indirect morphogenesis (via callus), were examined and their variability was assessed using two methods - RAPD and ISSR. A comparison of these methods was also made. Phylogenetic trees and networks were established and statistical analysis was performed. In order to obtain a more complete picture of the genetic differences among the P. zonale var. 'Kleiner Liebling' samples, other species belonging to the genus Pelargonium were also examined and their genetic variation was compared among themselves. The study showed that there was some genetic diversity in the P. zonale var. "Kleiner Liebling" samples despite their vegetative propagation. The percentage of monomorphic banding pattern for both methods was only 61%, but on the basis of trees and phylogenetic networks, these samples were combined into a single clade, and individual cytotypes did not group on separate branches. Regenerants showed relatively low variability, which may have been due to incomplete reproducibility of the methods used. No significant differences in genetic stability were observed between the regenerants of the two cytotypes, which could be due to differences in ploidy and the difference in the in vitro culture method of the two cytotypes. The other Pelargonium species studied were characterized by a large genetic distance and the exact phylogenetic relationships between them were not established, which may have been due to not enough data. The ISSR method showed slightly less polymorphism in the samples, but the RAPD method was characterized by longer reaction products and 7 a higher number of bands. The methods used in the study did not show significant differences in the reproducibility of the results.
Dostawca treści:: Repozytorium Uniwersytetu Jagiellońskiego

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Tytuł:: Preliminary studies on the molecular identification of sex in Taxus baccata L.
Autorzy:: Zarek, Marcin
Wydawca:: Instytut Badawczy Leśnictwa (Forest Research Institute), Sekocin Stary, Poland
Opis:: The scientific objective of this research was to screen random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) primers in order to find the molecular markers enabling the distinction between male and female individuals of the European yew. This is an initial step toward understanding the mechanisms of sex determination in this species. The study was conducted on European yew originating from two sites in Poland: the Zadni Gaj nature reserve near Cieszyn and the yew collection from the Botanical Garden of the Jagiellonian University in Krakow. In the present study, 716 random primers (696 RAPD primers: OPA -OPAI _1-16 and 20 ISSR primers marked as UBC) were tested to identify the sex in European yew by means of a modified bulked segregant analysis (BSA) method. The work was conducted in three stages, gradually limiting the number of primers through the elimination of primers that either did not exhibit any differences between the examined groups or did not provide amplification products. Among the tested primers, no ideal markers that would be present in all individuals of one sex but absent in the individuals of the other sex were found. However, some markers were found (A07_954, H13_729, J08_660, L12_390, U01_457, V14_527, AE03_941, AE03_1014) to occur with greater frequency in one sex. Using these, we further examined 13 band combinations (profiles) that were observed to occur only in male individuals and another 13 combinations that were observed only in female individuals, which could be used in the practical identification of sex. This is the first report to ascertain the sex of Taxus baccata trees, and it may help to determine the sex at an early stage of development.
Przemysław Szmit
Dostawca treści:: Repozytorium Centrum Otwartej Nauki

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Skocz do pozycji: 5.

Tytuł:: Report from the 33rd International Society for the Sociology of Religion Conference "Sensing Religion" in Louvain-la-Neuve (Belgium), 2–5 July 2015
Autorzy:: Niechciał, Paulina
Opis:: The report covers the 33rd International Conference organised by Society for the Sociology of Religion (ISSR; in French known as Société Internationale de Sociologie des Religions - SISR). The conference, themed "Sensing Religion", was held in Louvain-la-Neuve in Belgium, 2-5 July 2015.
Raport opowiada o 33. międzynarodowej konferencji, z cyklu organizowanego co dwa lata przez Society for the Sociology of Religion (ISSR; w języku francuskim Société Internationale de Sociologie des Religions - SISR). Konferencja zatytułowana "Sensing Religion" odbyła się Louvain-la-Neuve w Belgium, 2-5 lipca 2015 r.
Dostawca treści:: Repozytorium Uniwersytetu Jagiellońskiego

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Skocz do pozycji: 6.

Tytuł:: Organogenetic events in Rumex acetosa in vitro culture - histological studies and analysis of genetic variability.
Organogeneza w kulturach in vitro Rumex acetosa - badania histologiczne i ocena stabilności genetycznej regenerantów.
Autorzy:: Dziedzic, Katarzyna
Opis:: Celem niniejszej pracy było określenie typu morfogenezy w procesie mikrorozmnażania Rumex acetosa L. cv. Lioński na podstawie analizy histologicznej oraz ocena stabilności genetycznej regenerantów z zastosowaniem markerów molekularnych RAPD i ISSR. Kulturę hypokotyli pobranych z 11 – dniowych siewek prowadzono naMS + 0,5 mg/l TDZ. Kalogenezę zanotowano po 14 dniach prowadzenia hodowli, natomiast indukcję morfogenezy uzyskano po upływie 21 dni od założenia kultury.Analiza histologiczna przekrojów barwionych metodą PAS/NBB wykazała, że regeneracja roślin zachodziła na drodze organogenezy pośredniej (formowanie pędów przybyszowych via kalus). Centra merystematyczne zlokalizowane powierzchniowo i głębinowo w tkance kalusowej dawały początek regenerantom. Dodatkowo obserwowano organogenezę wtórną. Ocenę stabilności genetycznej roślin zregenerowanych in vitro przeprowadzono z zastosowaniem 10 starterów RAPD, które amplifikowały 124 prążki, 54 z nich były polimorficzne (43,55%). W przypadku 6 starterów ISSR otrzymano 73 prążki, spośród których 46 było polimorficznych (63%). Uzyskane wyniki wskazują na wysokie zróżnicowanie genetyczne roślin zregenerowanych in vitro.
The aim of the present work was to determine the type of morphogenesis during micropropagation of Rumex acetosa L. cv. Lionski by histological analysis and to assess genetic stability of regenerated plantlets using RAPD and ISSR molecular markers. The hypocotyls isolated from 11 – day – old seedlings were cultured on MS supplemented with 0,5 mg/l TDZ. The callogenesis was observed after 11 days from the beginning of the culture. Induction of morphogenesis was noticed after 21 days of culture.Histological analysis of tissue sections stained using periodic acid Schiff/naphthol blue black (PAS/NBB) revealed indirect organogenesis (adventitious shoots formation via callus). The regenerants were formed from meristematic centres located deep inside callus and near callus surface. Additionally, secondary organogenesis was observed. The genetic stability of in vitro regenerated plantlets was examined by 10 RAPD primers, which amplified 124 bands, 54 were polymorphic (43,55%). In case of 6 ISSR primers 73 bands were produced, 46 of them were polymorphic (63%). The results indicated high genetic diversity of in vitro regenerated plants.
Dostawca treści:: Repozytorium Uniwersytetu Jagiellońskiego

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Skocz do pozycji: 7.

Tytuł:: Genetic variability and virulence of some Iranian Rhizoctonia solani isolates associated with stem canker and black scurf of potato (Solanum tuberosum L.)
Autorzy:: Esfahani, M.N.
Tematy:: genetic diversity
isolates
ISSR
RAPD
virulence variability; Pokaż więcej
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:: https://bibliotekanauki.pl/articles/2084743.pdf Link otwiera się w nowym oknie
Opis:: Stem canker and black scurf of potato (Solanum tuberosum L.) caused by Rhizoctonia solani Kühn are important and epidemic diseases in potato-growing regions worldwide, including Iran. In this study, 120 isolates were retrieved from infected stem canker from six potato-growing regions in Iran (Isfahan, Ardebil, Fars, Hamedan, Kurdestan and Kerman). Out of these, 30 isolates were selected as representatives for genetic and virulence analysis. The isolates were analyzed by one sequence analyzes of the ITS-rDNA region, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), as well as virulence studies. Based on sequence analysis of the ITS-rDNA region, all 30 isolates were assigned to the anastomosis group (AG) and all were assigned to AG-3 PT. Cluster analysis using the unweighted pair group method with the arithmetic averages (UPGMA) method for both RAPD and ISSR markers revealed that they were divided into three main groups, with no correlation to geographical regions of the isolates. Pathogenicity tests showed that all isolates were pathogenic on potato cv. Agria; however, virulence variability was observed among the isolates. The grouping based on RAPD analysis and virulence variability was not correlated.
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Haploidyzacja żyta — diagnostyka molekularna oraz wpływ nanomolekuł na wspomaganie indukcji i regeneracji roślin w warunkach in vitro
Haploidization of the rye — the molecular diagnostics and the influence of nanomolecules on supporting the induction and regeneration of plants in in vitro conditions
Autorzy:: Mikołajczyk, Sylwia
Weigt, Dorota
Tomkowiak, Agnieszka
Broda, Zbigniew
Bocianowski, Jan
Tematy:: androgeneza
kultury pylników
markery ISSR i RAPD
żyto; Pokaż więcej
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Powiązania:: https://bibliotekanauki.pl/articles/2199538.pdf Link otwiera się w nowym oknie
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: MOLECULAR DIVERSITY AND PHYLOGENY OF TRITICUM-AEGILOPS SPECIES POSSESSING D GENOME REVEALED BY SSR AND ISSR MARKERS
Autorzy:: Moradkhani, Hoda
Mehrabi, Ali Ashraf
Etminan, Alireza
Pour-Aboughadareh, Alireza
Tematy:: Aegilops
genetic diversity
ISSR
molecular phylogeny
Triticum
SSR; Pokaż więcej
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Powiązania:: https://bibliotekanauki.pl/articles/2199633.pdf Link otwiera się w nowym oknie
Opis:: The aim of this study is investigation the applicability of SSR and ISSR markers in evaluating the genetic relationships in twenty accessions of Aegilops and Triticum species with D genome in different ploidy levels. Totally, 119 bands and 46 alleles were detected using ten primers for ISSR and SSR markers, respectively. Polymorphism Information Content values for all primers ranged from 0.345 to 0.375 with an average of 0.367 for SSR, and varied from 0.29 to 0.44 with the average 0.37 for ISSR marker. Analysis of molecular variance (AMOVA) revealed that 81% (ISSR) and 84% (SSR) of variability was partitioned among individu-als within populations. Comparing the genetic diversity of Aegilops and Triticum accessions, based on genetic parameters, shows that genetic variation of Ae. crassa and Ae. tauschii species are higher than other species, especially in terms of Nei’s gene diversity. Cluster analysis, based on both markers, separated total accessions in three groups. However, classification based on SSR marker data was not conformed to classification ac-cording to ISSR marker data. Principal co-ordinate analysis (PCoA) for SSR and ISSR data showed that, the first two components clarified 53.48% and 49.91% of the total variation, respectively. This analysis (PCoA), also, indicated consistent patterns of genetic relationships for ISSR data sets, however, the grouping of acces-sions was not completely accorded to their own geographical origins. Consequently, a high level of genetic diversity was revealed from the accessions sampled from different eco-geographical regions of Iran.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: MOLECULAR DIVERSITY AND PHYLOGENY OF TRITICUM-AEGILOPS SPECIES POSSESSING D GENOME REVEALED BY SSR AND ISSR MARKERS
Autorzy:: Moradkhani, Hoda
Mehrabi, Ali Ashraf
Etminan, Alireza
Pour-Aboughadareh, Alireza
Tematy:: Aegilops
genetic diversity
ISSR
molecular phylogeny
Triticum
SSR; Pokaż więcej
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Powiązania:: https://bibliotekanauki.pl/articles/55928227.pdf Link otwiera się w nowym oknie
Opis:: The aim of this study is investigation the applicability of SSR and ISSR markers in evaluating the genetic relationships in twenty accessions of Aegilops and Triticum species with D genome in different ploidy levels. Totally, 119 bands and 46 alleles were detected using ten primers for ISSR and SSR markers, respectively. Polymorphism Information Content values for all primers ranged from 0.345 to 0.375 with an average of 0.367 for SSR, and varied from 0.29 to 0.44 with the average 0.37 for ISSR marker. Analysis of molecular variance (AMOVA) revealed that 81% (ISSR) and 84% (SSR) of variability was partitioned among individu-als within populations. Comparing the genetic diversity of Aegilops and Triticum accessions, based on genetic parameters, shows that genetic variation of Ae. crassa and Ae. tauschii species are higher than other species, especially in terms of Nei’s gene diversity. Cluster analysis, based on both markers, separated total accessions in three groups. However, classification based on SSR marker data was not conformed to classification ac-cording to ISSR marker data. Principal co-ordinate analysis (PCoA) for SSR and ISSR data showed that, the first two components clarified 53.48% and 49.91% of the total variation, respectively. This analysis (PCoA), also, indicated consistent patterns of genetic relationships for ISSR data sets, however, the grouping of acces-sions was not completely accorded to their own geographical origins. Consequently, a high level of genetic diversity was revealed from the accessions sampled from different eco-geographical regions of Iran.
Dostawca treści:: Biblioteka Nauki

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