- Tytuł:
- Prediction of the structure of the common perimitochondrial localization signal of nuclear transcripts in yeast
- Autorzy:
-
Ejsmont, Radoslaw
Golik, Pawel
Stepien, Piotr - Tematy:
-
RNA trafficking
RNA structure
bioinformatics
mitochondria - Pokaż więcej
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Powiązania:
- https://bibliotekanauki.pl/articles/1041111.pdf Link otwiera się w nowym oknie
- Opis:
- Many nuclear genes encoding mitochondrial proteins require specific localization of their mRNAs to the vicinity of mitochondria for proper expression. Studies in Saccharomyces cerevisiae have shown that the cis-acting signal responsible for subcellular localization of mRNAs is localized in the 3' UTR of the transcript. In this paper we present an in silico approach for prediction of a common perimitochondrial localization signal of nuclear transcripts encoding mitochondrial proteins. We computed a consensus structure for this signal by comparison of 3' UTR models for about 3000 yeast transcripts with known localization. Our studies show a short stem-loop structure which appears in most mRNAs localized to the vicinity of mitochondria. The degree of similarity of a given 3' UTR to our consensus structure strongly correlates with experimentally determined perimitochondrial localization of the mRNA, therefore we believe that the structure we predicted acts as a subcellular localization signal. Since our algorithm operates on structures, it seems to be more reliable than sequence-based algorithms. The good predictive value of our model is supported by statistical analysis.
- Dostawca treści:
- Biblioteka Nauki
Artykuł