Temat: biogenesis - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: Clustered regularly interspaced short palindromic repeat (CRISPR) RNAs in the Porphyromonas gingivalis CRISPR-Cas I-C system
Autorzy:: Krochmal, Daniel
Staniec, Dominika
Pyrć, Krzysztof
Burmistrz, Michał
Martinez, Jose Ignacio Rodriguez
Dostawca treści:: Repozytorium Uniwersytetu Jagiellońskiego

Artykuł

na półce

Skocz do pozycji: 2.

Tytuł:: The Appearance of Life
Autorzy:: Krzymowski, Richard
Ługowski, Włodzimierz
Tematy:: Biogenesis
chemical evolution
pre-biological natural selection; Pokaż więcej
Wydawca:: Polska Akademia Nauk. Instytut Filozofii i Socjologii PAN
Powiązania:: https://bibliotekanauki.pl/articles/42563036.pdf Link otwiera się w nowym oknie
Opis:: The article presents the hypothesis of the origin of life. All the basic ideas of the 20th century research program in this area can be found there: the extrapolation of Darwinism into the area of chemical evolution, the influence of solar energy, the large “chemical possibilities” of carbon compounds, early heterotrophy, the gradual increase of organic purpose as a result of natural selection.
Dostawca treści:: Biblioteka Nauki

Artykuł

na półce

Skocz do pozycji: 3.

Tytuł:: Prohibitins and Ras2 protein cooperate in the maintenance of mitochondrial function during yeast aging.
Autorzy:: Kirchman, Paul
Miceli, Michael
West, Roger
Jiang, James
Kim, Sangkyu
Jazwinski, S
Tematy:: ROS
retrograde response
Saccharomyces cerevisiae
longevity
mitochondrial biogenesis; Pokaż więcej
Wydawca:: Polskie Towarzystwo Biochemiczne
Powiązania:: https://bibliotekanauki.pl/articles/1043375.pdf Link otwiera się w nowym oknie
Opis:: The yeast Saccharomyces cerevisiae has a finite replicative life span. Yeasts possess two prohibitins, Phb1p and Phb2p, in similarity to mammalian cells. These proteins are located in the inner mitochondrial membrane, where they are involved in the processing of newly-synthesized membrane proteins. We demonstrate that the elimination of one or both of the prohibitin genes in yeast markedly diminished the replicative life span of cells that lack fully-functional mitochondria, while having no effect on cells with functioning mitochondria. This deleterious effect was suppressed by the deletion of the RAS2 gene. The expression of PHB1 and PHB2 declined gradually up to 5-fold during the life span. Cells in which PHB1 was deleted in conjunction with the absence of a mitochondrial genome displayed remarkable changes in mitochondrial morphology, distribution, and inheritance. This loss of mitochondrial integrity was not seen in cells devoid of PHB1 but possessing an intact mitochondrial genome. In a subset of the cells, the changes in mitochondrial integrity were associated with increased production of reactive oxygen species, which co-localized with the altered mitochondria. The mitochondrial deficits described above were all suppressed by deletion of RAS2. Our data, together with published information, are interpreted to provide a unified view of the role of the prohibitins in yeast aging. This model posits that the key initiating event is a decline in mitochondrial function, which leads to progressive oxidative damage that is exacerbated in the absence of the prohibitins. This aggravation of the initial damage is ameliorated by the suppression of the production of mitochondrial proteins in the absence of Ras2p signaling of mitochondrial biogenesis.
Dostawca treści:: Biblioteka Nauki

Artykuł

na półce

Skocz do pozycji: 4.

Tytuł:: On the Theory of Prebiotic Evolution
Autorzy:: Rudenko, Alexander Prokofievich
Ługowski, Włodzimierz
Tematy:: biogenesis
theory of evolution of open catalytic systems; Pokaż więcej
Wydawca:: Polska Akademia Nauk. Instytut Filozofii i Socjologii PAN
Powiązania:: https://bibliotekanauki.pl/articles/42565016.pdf Link otwiera się w nowym oknie
Opis:: The main theorems of the theory of evolution of open catalytic systems are briefly presented. The difference between the “natural-historical” and “actualistic” approach to the problem of the origins of life was highlighted.
Dostawca treści:: Biblioteka Nauki

Artykuł

na półce

Skocz do pozycji: 5.

Tytuł:: Chromoplast biogenesis in Chelidonium majus petals
Autorzy:: Prebeg, T
Ljubesic, N.
Wrischer, M.
Tematy:: chromoplast
biogenesis
Chelidonium majus
petal
greater celandine
fibrillin
ultrastructure; Pokaż więcej
Wydawca:: Polskie Towarzystwo Botaniczne
Powiązania:: https://bibliotekanauki.pl/articles/57510.pdf Link otwiera się w nowym oknie
Opis:: The differentiation of chromoplasts, with special emphasis on the formation and the organisation of chromoplast fibrils, was followed in the petals of the greater celandine, Chelidonium majus L. Electron microscopic observations showed that, in the epidermis, differentiation of chromoplasts started from leucoplasts, while mesophyll chromoplasts originated from chloroplasts. During petal maturation, fibrils accumulated in the plastids, often arranging in a parallel fashion to form compact birefringent bundles. Immediately before flower opening, these fibrillar bundles started to disorganise, and, at anthesis, most chromoplasts contained widely spaced fibrils which were irregularly dispersed through the plastid interior. During chromoplast differentiation, fibrils were commonly observed to protrude from plastoglobules, suggesting the possible site of their formation. Western analysis indicated that a protein antigenically related to fibrillin from pepper chromoplasts participates in the constitution of fibrils in Chelidonium petals.
Dostawca treści:: Biblioteka Nauki

Artykuł

na półce

Skocz do pozycji: 6.

Tytuł:: Nuclear and mitochondrial genome responses in HeLa cells treated with inhibitors of mitochondrial DNA expression
Autorzy:: Piechota, Janusz
Szczęsny, Roman
Wolanin, Kamila
Chlebowski, Aleksander
Bartnik, Ewa
Tematy:: thiamphenicol
mtDNA depletion
HeLa
dideoxycytidine
mitochondrial biogenesis
ethidium bromide; Pokaż więcej
Wydawca:: Polskie Towarzystwo Biochemiczne
Powiązania:: https://bibliotekanauki.pl/articles/1041204.pdf Link otwiera się w nowym oknie
Opis:: The influence of mutations in the mitochondrial DNA (mtDNA) on the bioenergetic metabolism of the cell is still poorly understood. Many of the mutations in the mtDNA affect the expression of the mitochondrial genome. Investigations on cells from patients are not easy, especially as the mitochondrial DNA is heteroplasmic and this state is changed in culture. Moreover, the nuclear background and the mitochondrial haplotype may affect the behaviour of cells. Transfer of patient mitochondria to rho zero cell lines is also not optimal as these cells in general have many nuclear changes which may also affect cell behaviour. Thus, we decided to use inhibitors of mitochondrial genome expression, such as thiamphenicol, ethidium bromide and dideoxycytidine to investigate the bioenergetic metabolism of HeLa cells. We found that oxidative phosphorylation and glycolysis participate equally in ATP production in HeLa cells and that decreased activity of the respiratory chain leads to increased glycolysis and the reduction of cell growth. Insufficient ATP production in the oxidative phosphorylation process was not compensated by increased proliferation of the mitochondria. However, we were able to show that there are some mechanisms compensating limited expression of the mitochondrial genome within the mitochondria. Experiments with dideoxycytidine revealed that 10-fold decrease of the mtDNA copy number resulted in almost normal activity of cytochrome c oxidase. We found that mtDNA depletion is compensated mostly on the level of RNA metabolism in the mitochondria. Thus, our results are in agreement with the hypothesis that transcription initiation rather than mtDNA copy number is a rate limiting factor for expression of the mitochondrial genome.
Dostawca treści:: Biblioteka Nauki

Artykuł

na półce

Skocz do pozycji: 7.

Tytuł:: Discrete dynamic system oriented on the formation of prebiotic dipeptides from Rodes experiment
Autorzy:: Polanco, Carlos
Samaniego, José
Buhse, Thomas
Castañón González, Jorge
Tematy:: origins of life
biogenesis
dipeptides
salt-induced peptide formation; Pokaż więcej
Wydawca:: Polskie Towarzystwo Biochemiczne
Powiązania:: https://bibliotekanauki.pl/articles/1039201.pdf Link otwiera się w nowym oknie
Opis:: This work attempts to rationalize the possible prebiotic profile of the first dipeptides of about 4 billion years ago based on a computational discrete dynamic system that uses the final yields of the dipeptides obtained in Rode's experiments of salt-induced peptide formation (Rode et al., 1999, Peptides 20: 773-786). The system built a prebiotic scenario that allowed us to observe that (i) the primordial peptide generation was strongly affected by the abundances of the amino acid monomers, (ii) small variations in the concentration of the monomers have almost no effect on the final distribution pattern of the dipeptides and (iii) the most plausible chemical reaction of prebiotic peptide bond formation can be linked to Rode's hypothesis of a salt-induced scenario. The results of our computational simulations were related to former simulations of the Miller, and Fox & Harada experiments on amino acid monomer and oligomer generation, respectively, offering additional information to our approach.
Dostawca treści:: Biblioteka Nauki

Artykuł

na półce

Skocz do pozycji: 8.

Tytuł:: Synthesis of Zinc oxide nanoparticles from Clitoria ternatea L. extracts: a green approach
Autorzy:: Manokari, M.
Shekhawat, Mahipal S.
Tematy:: Biogenesis
Zinc oxide nanoparticles
Clitoria ternatea
UV-Visible characterization; Pokaż więcej
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Powiązania:: https://bibliotekanauki.pl/articles/1189980.pdf Link otwiera się w nowym oknie
Opis:: We report the development of green methods for synthesis of zinc oxide at nano scale. Biological systems are being explored for the synthesis of nanoparticles. The plant Clitoria ternatea contains alkaloids, tannins, glycosides, resins, steroids, saponins, flavonoids, phenols, kaempferol, clitorin, taraxerol, aparajitin, sistosterol, hexacosanal and anthoxanthin. The whole plant is traditionally used to treat leprosy, bronchitis, inflammation, leucoderma and tuberculosis. The present report describes the biosynthesis and characterization of zinc oxide nanoparticles using aqueous extracts of leaves, stem, root, flowers and fruits of Clitoria ternatea. The aqueous reaction mixture of leaf, flower petals and roots showed strong broad UV spectral peak at 296 nm, stem at 293 nm and roots at 311 nm.
Dostawca treści:: Biblioteka Nauki

Artykuł

na półce

Skocz do pozycji: 9.

Tytuł:: Nowożytne przemiany idei samorództwa
Modern Transformations of the Idea of Autogeny
Autorzy:: Świeżyński, Adam
Tematy:: biogeneza
samorództwo
mechanicyzm
witalizm
mikroorganizmy
biogenesis
autogeny
mechanicism
vitalism
microorganisms; Pokaż więcej
Wydawca:: Katolicki Uniwersytet Lubelski Jana Pawła II. Towarzystwo Naukowe KUL
Powiązania:: https://bibliotekanauki.pl/articles/2013051.pdf Link otwiera się w nowym oknie
Opis:: Autogeny, sometimes also called “naïve”, spontaneous generation (Lat. generatio spontanea) is – most generally speaking – a view, according to which living creatures come into being spontaneously and voluntarily from inanimate matter. Such a broad formulation of the idea of autogeny, however, does not show significant differences that occur in understanding it. The basis for these differences is constituted by different definitions of what should be considered inanimate matter, and what – animate matter. A thorough consideration of the arguments that have been offered in the history of research into the nature in order to justify the idea of autogeny, and an investigation into the modern debates and controversies concerning this idea allow discovering a variety of interpretations of the view of a spontaneous and voluntary origin of biological organisms. The variety was formed together with the development of the scientific empirical method and with the participation of philosophical concepts explaining the way the animate world functions. With time, the idea of a spontaneous origin of organisms underwent many transformations, first consisting in limiting the range of its application (from macroscopic organisms with complex structure to relatively simple microorganisms), then in a change in its understanding, and finally to questioning the very idea. Also the way changed, in which the possibility of the occurrence of autogeny in the nature was motivated. However, it seems that the very core of the idea of autogeny, which contains a general thought about transformation of matter leading to the origin of living organisms is still maintained in the contemporary natural science.
Dostawca treści:: Biblioteka Nauki

Artykuł

na półce

Skocz do pozycji: 10.

Tytuł:: Rozwojowo-zależna indukcja biogenezy mitochondriów podczas różnicowania neuralnego ludzkich indukowanych pluripotencajalnych komórek macierzystych (hiPSC)
Autorzy:: Augustyniak, Justyna
Współwytwórcy:: Bużańska, Leonora
Wydawca:: Instytut Medycyny Doswiadczalnej i Klinicznej im. M. Mossakowskiego PAN
Opis:: Human induced pluripotent stem cells (hiPSC) generated from somatic cells through genetic reprogramming influenced greatly development of basic research in regenerative medicine as well as in vitro toxicology and pharmacology field. The progress in the “safemethods” of hiPSC generation (without the integration of the transgene into the host genome, eg., mRNA, recombinant proteins, miRNA’s, episomal vectors) gave an opportunity to use this cells in personalized cell therapy. In addition, the hiPSC serve as ethically non-controversial in vitro model of early human development which is an alternative to the model of human embryonic stem cells (hESC). The cycle of publications chosen for the theses investigates the influence of stimulation of mitochondrial biogenesis on the early stages of hiPSC neural differentiation. In this study, neural differentiation of hiPSC resulted in obtaining three distinct cells populations: neural stem cells (NSC), early neural progenitors (eNP), and neural progenitors (NP) however, the population of eNP cells has been characterized for the first time. Analysis of the gene and protein expression have shown that NSC, eNP and NP cell populations were significantly different in the level of unique markers for early neural development. The obtained cell populations were investigated for their sensitivity to compounds stimulating the mitochondrial biogenesis: Pyrroloquinoline quinone (PQQ) or idebenone (IDB), which were added independently. The results revealed significant changes in the cells viability, free radical level (ROS) and mitochondrial membrane potential (ΔΨm) upon the treatment with PQQ and IDB in all tested populations. The expression of genes related with mitochondrial biogenesis regulation: NRF1, PPARGC1A andTFAM were also significantly different. However exclusively at the eNP stage, after incubation with PQQ and IDB, all markers indicating stimulation of mitochondrial biogenesis were significantly elevated.This included upregulation of NRF1, PPARGC1A, TFAM gene expression, increased number of copies of mitochondrial DNA (mtDNA) and significant elevation of expression of proteinsimportant for mitochondrial function: COX-1 and SDHA. Gene expression analysis of neural differentiation upon PQQ treatment revealed in NSC and eNP stages of developmentsimultaneous increase in expression of PPARGC1A (main regulator of mitochondrial biogenesis) and astrocyte marker GFAPaccompanied with repression of the neuronal marker MAP2. IDB in all stages of development yielded a similar effect with the exception of eNP, where stimulation of theexpression of both GFAP and MAP2was observed, although the increase in GFAP expression was higher. The above data demonstrate the existence of developmental “window of sensitivity" for investigated factors (PQQ, IDB) inducing mitochondrial biogenesis at eNP stage of development and the possibility of influencing of the neural differentiation pathways via PQQ and IDB in favour of astrocytic fate.
Biblliografia zawiera 40 pozycji.
95 s.: il., wykr.tabl., fotogr. ; 30 cm.
Odkrycie, że komórki somatyczne można reprogramować do indukowanych pluripotencjalnych komórek macierzystych (iPSC, ang. induced Pluripotent Stem Cells) pozwoliło na znaczny rozwójbadań w dziedzinie medyczny regeneracyjnej, jak również badań toksykologicznych oraz farmakologicznych in vitro. Postęp w otrzymywaniu ludzkich iPSC (hiPSC,ang. human iPSC) tzw. „bezpiecznymi metodami” tj. bez integracji transgenu do genomu gospodarza (np. stosując mRNA, białka rekombinowane, miRNA, wektory episomalne) przyczynił się do wykorzystania tych komórek w spersonalizowanej terapii komórkowej. Dodatkowo hiPSC stanowią „niekontrowersyjną etycznie”alternatywę dla ludzkich zarodkowych komórek macierzystych (hESC,ang. human Embryonic Stem Cells) w modelowaniu in vitrowczesnych etapów rozwojuczłowiekadzięki zdolności doróżnicowania we wszystkie tkanki organizmu. W cyklu publikacji stanowiących rozprawędoktorskąprzedstawiono wyniki oceny wpływu stymulacji biogenezy mitochondriów na wczesne etapy różnicowania neuralnego komórek hiPSC. W tym celu z hiPSC otrzymano trzy populacje komórkowe: neuralne komórki macierzyste (NSC, ang. Neural Stem Cells), wczesne progenitory neuralne (eNP, ang. early Neural Progenitors) oraz progenitory neuralne (NP, ang. Neural Progenitors), przy czym komórki eNP scharakteryzowano po raz pierwszy. Badane populacje komórkowe różniłysię istotnie pod względem ekspresjimarkerów typowych dla rozwoju neuralnego na poziomie mRNA i białek. Komórki NSC, eNP oraz NP zostały poddane ekspozycji na substancje indukujące biogenezę mitochondriów: pirolochinolinochinon (PQQ) lub idebenon (IDB). Pod wpływem tych związków wykazano istotne zmiany w parametrach ważnych dlaprocesów życiowychkomórki: żywotności, poziomie wolnych rodników (RFT), potencjale błony mitochondrialnej (ΔΨm) oraz ekspresji genów związanych z regulacją biogenezy mitochondriów: NRF1, PPARGC1A, TFAM. Wzrostowi ekspresji tych genów towarzyszył wzrostpozostałych badanych markerów biogenezy mitochondriów: liczby kopii mitochondrialnego DNA (mtDNA) oraz ekspresji białek SDHA i COX-1, wyłącznie w stadium eNP. W komórkach NSC i eNP, po inkubacji z PQQ zanotowano równoczesny wzrost ekspresji genu PPARGC1Aoraz markera astrocytów GFAP, przy jednoczesnym spadku ekspresji markera neuronalnego MAP2. IDB działał podobnie we wszystkich stadiach, z wyjątkiem eNP, gdzie stymulował ekspresję zarówno GFAP jak i MAP2. Powyższe wyniki świadczą o istnieniu w stadium eNP „przedziału wrażliwości rozwojowej” na PQQ oraz IDB, a także o pozytywnym wpływie stymulacji biogenezy mitochondriów na różnicowanie komórek hiPSC w kierunku astrocytarnym.
Dostawca treści:: RCIN - Repozytorium Cyfrowe Instytutów Naukowych

Inne

na półce

Informacja

Wyszukujesz frazę "biogenesis" wg kryterium: Temat