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Wyświetlanie 1-10 z 96
Tytuł:
Bacterial putative metacaspase structure from Geobacter sulfureducens as a template for homology modeling of type II Triticum aestivum metacaspase (TaeMCAII)
Autorzy:
Dudkiewicz, Malgorzata
Piszczek, Ewa
Tematy:
Programmed Cell Death
metacaspase
Pokaż więcej
Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1039720.pdf  Link otwiera się w nowym oknie
Opis:
Metacaspases, cysteine proteases belonging to the peptidase C14 family, are suspected of being involved in the programmed cell death of plants, although their sequences and substrate specificity differ from those of animal caspases. At present, the knowledge on the metacaspase reaction mechanism is based only on biochemical data and homology models constructed on caspase templates. Here we propose a novel template for metacaspase modeling and demonstrate important advantages in comparison to the conventionally used caspase templates. We also point out the connection between plant and bacterial metacaspases, underlining the prokaryotic roots of Programmed Cell Death (PCD).
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mouse Pxt1 expression is regulated by Mir6996 miRNA
Autorzy:
Rokicki, Mikołaj
Sieńko, Wioleta
Nalepa, Anna
Tomczyk, Igor
Wiench, Jasmin
Grzmil, Paweł
Rożek, Katarzyna
Opis:
Mouse Pxt1 gene is expressed exclusively in male germ cells and encodes for a small, cell death inducing protein. However, upon PXT1 interaction with BAG6, cell death is prevented. In transiently transfected cell lines the PXT1 expression triggered massive cell death, thus we ask the question whether the interaction of PXT1 and BAG6 is the only mechanism preventing normal, developing male germ cells from being killed by PXT1. The Pxt1 gene contains a long 3′UTR thus we have hypothesized that Pxt1 can be regulated by miRNA. We have applied Pxt1 knockout and used Pxt1 transgenic mice that overexpressed this gene to shed more light on Pxt1 regulation. Using the ELISA assay we have demonstrated that PXT1 protein is expressed in adult mouse testis, though at low abundance. The application of dual-Glo luciferase assay and the 3′UTR cloned into p-MIR-Glo plasmid showed that Pxt1 is regulated by miRNA. Combining the use of mirDB and the site-directed mutagenesis further demonstrated that Pxt1 translation is suppressed by Mir6996-3p. Considering previous reports and our current results we propose a model for Pxt1 regulation in the mouse male germ cells.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Tytuł:
Curcumin induces cell death without oligonucleosomal DNA fragmentation in quiescent and proliferating human CD8+ cells
Autorzy:
Magalska, Adriana
Brzezinska, Agnieszka
Bielak-Zmijewska, Anna
Piwocka, Katarzyna
Mosieniak, Grażyna
Sikora, Ewa
Tematy:
CD8+
cell death
DNA degradation
curcumin
Pokaż więcej
Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1041209.pdf  Link otwiera się w nowym oknie
Opis:
Cytotoxic CD8+ cells play an important role in determining host response to tumor, thus chemotherapy is potentially dangerous as it may lead to T cells depletion. The purpose of this study was to elucidate the propensity of quiescent and proliferating human CD8+ cells to undergo cell death upon treatment with curcumin, a natural dye in Phase I of clinical trials as a prospective chemopreventive agent. Methods: We treated human quiescent or proliferating CD8+ cells with 50 µM curcumin or irradiated them with UVC. Cell death symptoms such as decreased cell viability, chromatin condensation, activation of caspase-3 and specific DFF40/CAD endonuclease and oligonucleosomal DNA fragmentation were analyzed using MTT test, microscopic observation, Western blotting and flow cytometry. Results: Curcumin decreased cell viability, activated caspase-3 and decreased the level of DFF45/ICAD, the inhibitor of the DFF40/CAD endonuclease. However, this did not lead to oligonucleosomal DNA degradation. In contrast, UVC-irradiated proliferating, but not quiescent CD8+ cells revealed molecular and morphological changes characteristic for apoptosis, including oligonucleosomal DNA fragmentation. Curcumin can induce cell death in normal human lymphocytes both quiescent and proliferating, without oligonucleosomal DNA degradation which is considered as a main hallmark of apoptotic cell death. Taking into account the role of CD8+ cells in tumor response, their depletion during chemotherapy could be particularly undesirable.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dendritic cell-based immunotherapy (DCVAC/OvCa) combined with second-line chemotherapy in platinum-sensitive ovarian cancer (SOV02) : A randomized, open-label, phase 2 trial
Autorzy:
Pluta, Marek
Galluzzi, Lorenzo
Minar, Lubos
Hrnciarova, Tereza
Melichar, Bohuslav
Spacek, Jiri
Madry, Radoslaw
Rob, Lukas
Fucikova, Jitka
Streb, Joanna
Chovanec, Josef
Cibula, David
Hassan, Hariz Iskandar Bin
Pecen, Ladislav
Valha, Petr
Mallmann, Peter
Wimberger, Pauline
Kieszko, Dariusz
Spisek, Radek
Hein, Alexander
Markowska, Janina
Knapp, Pawel
Klat, Jaroslav
Hraska, Marek
Bartos, Pavel
Bartunkova, Jirina
Opis:
Objective: DCVAC/OvCa is an active cellular immunotherapy designed to stimulate an immune response against ovarian cancer. We explored the safety and efficacy of DCVAC/OvCa plus carboplatin and gemcitabine in platinum-sensitive ovarian cancer.:Methods: In this open-label, parallel-group, phase 2 trial (ClinicalTrials.gov number NCT02107950), patients with platinum-sensitive ovarian cancer relapsing after first-line chemotherapy were randomized to DCVAC/OvCa and chemotherapy or chemotherapy alone. DCVAC/OvCa was administered every 3–6 weeks (10 doses). Endpoints included safety, progression-free survival (PFS; primary efficacy endpoint) and overall survival (OS; secondary efficacy endpoint). Results: Between November 2013 and May 2015, 71 patients were randomized to chemotherapy in combination with DCVAC/OvCa or to chemotherapy alone. Treatment-emergent adverse events related to DCVAC/OvCa, leukapheresis and chemotherapy occurred in six (16.2%), two (5.4%), and 35 (94.6%) patients in the DCVAC/OvCa group. Chemotherapy-related events occurred in all patients in the chemotherapy group. Seven patients in the DCVAC/OvCa group were excluded from primary efficacy analyses due to failure to receive ≥1 dose of DCVAC/OvCa. PFS was not improved (hazard ratio [HR] 0.73, 95% confidence interval [CI] 0.42–1.28, P = 0.274, data maturity 78.1%). Median OS was significantly prolonged (by 13.4 months) in the DCVAC/OvCa group (HR 0.38, 95% CI 0.20–0.74, P = 0.003; data maturity 56.3%). A signal for enhanced surrogate antigen-specific T-cell activity was seen with DCVAC/OvCa. Conclusions" DCVAC/OvCa combined with chemotherapy had a favorable safety profile in patients with platinum-sensitive ovarian cancer. DCVAC/OvCa did not improve PFS, but the exploratory analyses revealed OS prolongation and enhanced surrogate antigen-specific T-cell activity.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Tytuł:
Rola ceramidu w molekularnych mechanizmach śmierci komórek ludzkiej neuroblastoma SH-SY5Y. Analiza działania wybrania cytoprotektantów
Autorzy:
Czubowicz, Kinga
Współwytwórcy:
Strosznajder, Robert
Wydawca:
Medical Research Center Polish Academy of Sciences
Instytut Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego PAN
Powiązania:
Praca doktorska
Opis:
Bibliogr. na str. 92-120
120 s.: il., wykr., fotogr. ; 30 cm
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Inne
Autorzy:
Płonczyńska, Alicja
Potempa, Jan
Sochalska, Maja
Prucsi, Zsombor
Opis:
Periodontitis (PD) is a chronic inflammatory disease that affects a significant portion of the global population. In susceptible individuals, the disease is driven by dysbiotic microbiota on the tooth surface below the gum line, progressively eroding the tooth-supporting structures of the periodontium, including the alveolar bone. Clinically, PD manifests as attachment loss and periodontal pocket formation. Influenced by environmental factors, it can ultimately lead to tooth loss and is associated with an increased risk of systemic conditions. Host cells, including oral keratinocytes, gingival fibroblasts, and monocytes/macrophages, regulate the immune response that drives chronic inflammation and tissue damage in PD. Programmed cell death pathways - apoptosis, pyroptosis, and necroptosis - are key regulators of the immune response. These pathways orchestrate the elimination of infected, activated, and/or damaged cells, which is essential for either fuelling or resolving local inflammation. However, periodontal pathogens, particularly Porphyromonas gingivalis, can manipulate these pathways, supporting the maintenance of highly inflammatory environment. Prolonged exposition to proinflammatory agents may induce cellular senescence. This process contributes to chronic inflammation and tissue breakdown, further exacerbating the progression of PD. In this review, we discuss the key factors contributing to the onset and progression of PD, the virulence factors of P. gingivalis, and their effects on immune responses and cell death in keratinocytes, gingival fibroblasts, and macrophages.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Tytuł:
Interplay between different cytotoxic parameters in Galleria mellonella (Lepidoptera, Pyralidae) larvae fed with polypropylene
Autorzy:
Homa, Joanna
Wilczek, G.
Chajec, Ł.
Siwek, D.
Rost-Roszkowska, M.
Wrońska, A. K.
Student, S.
Mermer, P.
Krpec, K.
Magiera, K.
Opis:
Plastic waste, which pollutes water and soil and negatively impacts organisms, is currently a major ecological problem. Therefore, methods for its degradation are being sought, including biodegradation using various organisms to dispose of plastics. One invertebrate animal suspected of being used in plastic biodegradation is Galleria mellonella (Insecta, Lepidoptera). However, there is no data on whether plastics ingested by this insect’s larvae will induce cytotoxic effects in cells, tissues, or organs, which would exclude this species from biodegradation. The aim of this study was to determine whether G. mellonella larvae, after consuming a popular plastic, polypropylene (PP), activate specific cytotoxic parameters. Larvae of the studied species were fed PP bags for 24 and 48 h. Control (G0-C) and starved (G0-S) individuals were also analyzed to determine whether cytotoxic effects could be attributed to factors such as a lack of normal food. Confocal microscopy and flow cytometry were changes, employed to investigate cell death processes, caspase and Bcl-2 protein activation, and mitochondrial alterations. The results of our studies suggest that G. mellonella may be considered as a potential candidate used in the biodegradation of PP.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Wyświetlanie 1-10 z 96

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