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Wyszukujesz frazę "enzymatic activity" wg kryterium: Temat


Tytuł:
Soil Redox Potential and its Impact on Microorganisms and Plants of Wetlands
Autorzy:
Tokarz, E.
Urban, D.
Tematy:
redox potential
peatland
microbial communities
enzymatic activity
Pokaż więcej
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Powiązania:
https://bibliotekanauki.pl/articles/125438.pdf  Link otwiera się w nowym oknie
Opis:
Although peatlands cover only 3% of the Earth’s surface, they constitute a huge reservoir of carbon. It is estimated that they accumulate one third of carbon contained in all types of soils worldwide. Therefore, knowledge of the physical, chemical, and biological properties of peat is important for prevention of peat degradation and release of carbon stored as CO2 into the atmosphere. In organic soils, water plays a very important role as a protective factor against mineralisation of organic matter. Therefore, organic soils are characterised by high specificity and dissimilarity from mineral soils. The hydrological factor induces a variety of changes in the physical and chemical properties, e.g. low redox potential or low oxygen content in soil pores. Many soil processes are determined by the soil oxygenation status, which can be measured with various indicators as well as direct and indirect measurements. One of the indirect methods is measurement of the redox potential. The oxidation-reduction potential (redox potential or Eh) is a measure of the ratio of oxidised to reduced forms in a solution. This parameter is inextricably linked to oxygen supply and the processes of consumption thereof by microorganisms and plant roots. Therefore, the redox potential is used as an indicator of the oxygenation status and the content of biogenic forms and toxins in the soil environment and sediments. In the case of submerged soils, penetration of atmospheric oxygen into the soil is limited due to low rates of oxygen diffusion and, hence, low redox potential, which inhibits plant growth through inhibition of respiration and production of toxins in reducing conditions. The aim of this article is (1) to the show soil-plant-soil microorganism interactions taking place on peatbogs in the context of redox potential, (2) to investigate the responses of plants and soil microorganisms to the changing redox potential, and (3) to demonstrate the mechanisms of plant adaptation to the reducing conditions prevailing in peatbogs.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protection
Autorzy:
Łopusiewicz, Ł.
Mazurkiewicz-Zapałowicz, K.
Tkaczuk, C.
Bartkowiak, A.
Tematy:
cobalt
entomopathogenic fungi
enzymatic activity
heavy metals
Pokaż więcej
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/2084794.pdf  Link otwiera się w nowym oknie
Opis:
This study focused on the effect of heavy metal cobalt ions (at concentrations of 1–1000 ppm) on the development and enzymatic activity of four entomopathogenic fungi: Beauveria bassiana, Beauveria brongniartii, Isaria fumosorosea and Metarhizium robertsii, commonly used in biological plant protection. It was found that each of the tested species of fungi reacted individually to contact with the Co2+ ions at their various concentrations. Depending on the variants of the experiment carried out, there were changes in the development of the mycelia (mainly growth inhibition) and their morphological features (color and structure) in comparison to the control samples. Co2+ ions had a fungistatic effect on all fungal strains, whereas a fungicidal effect was noted at concentrations of 750 ppm and 1000 ppm against M. robertsii and I. fumosorosea, respectively. In addition, there was a discrepancy in enzymatic activity between the tested fungal species developing in the medium with varying concentrations of metal salt. The inhibitory effect of Co2+ ions on lipase production was observed in I. fumosorosea. Protease production was stimulated in B. bassiana at all Co2+ concentrations, whereas in M. robertsii this effect was noted at 1 ppm. The changing dynamics of extracellular fungal hydrolases, due to the action of Co2+ ions, may translate into the role of these microorganisms in the processes of insect pathogenesis. This work suggests that severe pollution of the environment by cobalt could be a restrictive factor for the development and pathogenicity of entomopathogenic fungi and must be taken into account for their successful application in biological plant protection.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of wheat and Miscanthus straw biochars on soil enzymatic activity, ecotoxicity, and plant yield
Autorzy:
Mierzwa-Hersztek, Monika
Gondek, Krzysztof
Klimkowicz-Pawlas, Agnieszka
Baran, Agnieszka
Tematy:
biochar
soil
enzymatic activity
bioassays
crop yield
Pokaż więcej
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Powiązania:
https://bibliotekanauki.pl/articles/973069.pdf  Link otwiera się w nowym oknie
Opis:
The variety of technological conditions and raw materials from which biochar is produced is the reason why its soil application may have different effects on soil properties and plant growth. The aim of this study was to evaluate the effect of the addition of wheat straw and Miscanthus giganteus straw (5 t DM ha-1) and biochar obtained from this materials in doses of 2.25 and 5 t DM ha-1 on soil enzymatic activity, soil ecotoxicity, and plant yield (perennial grass mixture with red clover). The research was carried out under field conditions on soil with the granulometric composition of loamy sand. No significant effect of biochar amendment on soil enzymatic activity was observed. The biochar-amended soil was toxic to Vibrio fischeri and exhibited low toxicity to Heterocypris incongruens. Application of wheat straw biochar and M. giganteus straw biochar in a dose of 5 t DM ha-1 contributed to an increase in plant biomass production by 2 and 14%, respectively, compared to the soil with mineral fertilisation. Biochars had a more adverse effect on soil enzymatic activity and soil ecotoxicity to H. incongruens and V. fischeri than non-converted wheat straw and M. giganteus straw, but significantly increased the grass crop yield.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Resistance of mosses to drying, measured by the intensity of gas exchange and the content of malate and citrate
Autorzy:
Rzepka, Andrzej
Szarek, Wioleta
Tematy:
dehydration and rehydration
enzymatic activity
photosynthesis
respiration
Pokaż więcej
Wydawca:
Uniwersytet Pedagogiczny im. Komisji Edukacji Narodowej w Krakowie
Powiązania:
https://bibliotekanauki.pl/articles/2173118.pdf  Link otwiera się w nowym oknie
Opis:
Desiccation tolerance, the ability to lose virtually all of its free intracellular water and then restore normal function when rehydrated, is one of the most remarkable features of bryophytes. The aim of the study was to determine the resistance of two species of Plagiomnium undulatum (Hedw.) T.J.Kop. and Polytrichum commune Hedw. on drying to 50% relative water content of the air and rehydration. Changes in the intensity of photosynthesis and respiration as well as the content of malate and citrate in leafy moss stems were analysed. P. commune gametophores showed greater resistance to drought stress than P. undulatum. In both species, photosynthesis was much more sensitive to drought than respiration. Changes in the content of malate and citrate indicated a high plasticity of moss metabolism in conditions of water shortage and may be one of many important elements of the adaptation strategy to water deficit. The reactions of the tested mosses to dehydration and rehydration confirmed their adaptation to specific land conditions.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Leaf litterfall decomposition of pedunculate (Quercus robur L.) and sessile (Q. petraea [Matt.] Liebl.) oaks and their hybrids and its impact on soil microbiota
Autorzy:
Jurkšienė, Girmantė
Janušauskaitė, Dalia
Armolaitis, Kęstutis
Baliuckas, Virgilijus
Tematy:
biochemical properties
enzymatic activity
metabolic activity
microorganisms
microbial communities
Pokaż więcej
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Powiązania:
https://bibliotekanauki.pl/articles/956787.pdf  Link otwiera się w nowym oknie
Opis:
Trakas Forest is the only natural habitat of sessile oak in Lithuania. Sessile oak stand here is growing about 60–70 km from the nearest natural sessile oak stands in Poland. The purpose of this study was to determine whether autumn leaf litterfall of pedunculate and sessile oaks and their hybrids have different biochemical composition and decomposition rate and, consequently, different impacts on microbial condition of rhizosphere. For this purpose in autumn leaf litterfall C, N, P, K, Ca, Mg, lignin, ash, fat, crude fibre and water-soluble carbohydrates contents and stocks, lignin/N, lignin/P, C/N, C/P, N/P ratios, the decomposition rate and CO2 emissions were determined. In rhizosphere of studied oak species N, C concentration, pHH2O, C/N ratio, and dehydrogenase, urease, phosphatase, bacteria and micromycetes amount were estimated as well. The litterfall of pedunculate oak was distinguished by a higher content of lignin, higher lignin/N ratio, lower decay rate and lower carbon release, which determines decreased activity of micromycetes in the rhizosphere. Metabolic activity of microorganisms differed insignificantly among tree species rhizospheres. However, the potential for the use of carbon compound substrates and biodiversity index have a tendency to be higher in the soil under sessile oak. Lower decomposition rate of leaf litterfall and organic compounds in the rhizosphere under pedunculate oak allowed to suppose that the conditions for natural regeneration were more suitable in stands where sessile and hybrid oaks dominate.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of soil quality after biochar application based on enzymatic activity and microbial composition
Autorzy:
Mierzwa-Hersztek, M.
Gondek, K.
Klimkowicz-Pawlas, A.
Chmiel, M.J.
Dziedzic, K.
Taras, H.
Tematy:
biochar
soil
enzymatic activity
microorganisms
soil quality indexes
Pokaż więcej
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Powiązania:
https://bibliotekanauki.pl/articles/2082557.pdf  Link otwiera się w nowym oknie
Opis:
Soil microorganisms play a key role in many biochemical processes essential for the environment and for the ecological and production functions of soils, hence they are very important quality indicators. The study aimed at evaluating the effect of 1 and 2% additions of wheat and miscanthus straw biochars on soil enzymatic activity (dehydrogenases, urease, phosphatases) and the number of microorganisms (bacteria, fungi, actinobacteria). Pot experiments were carried out on soil with a loamy sand texture. The geometric mean of enzyme activities, biological index of fertility, and the integrated total enzyme activity index were used to explore the relationships between soil enzyme activities and the microbiological and chemical properties of soil. The addition of 1% miscanthus straw biochar had the most beneficial effect on the number of bacteria and fungi (increase by 380 and 26%, respectively), and 1% wheat straw biochar on the number of actinomycetes (increase by 273%). The correlation analysis between the number of actinomycetes and the chemical parameters of the soil showed the significant effect of the content of Ntotal (r = 0.76) and Ctotal (r = 0.85). The values of biological index of fertility, total enzyme activity index and geometric mean of enzyme activities indexes showed that the best quality of soil was produced by a 2% addition of wheat straw biochar.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nitrate Reductase Activity in Eucalyptus urophylla and Khaya senegalensis Seedlings: Optimization of the in vivo Assay
Autorzy:
Barbosa, Mateus Pires
do Bonfim, Raul Antonio Araújo
da Silva, Leandro Dias
Souza, Mikaela Oliveira
de Souza Soares, Poliana Prates
Sá, Milton Carriço
Cairo, Paulo Araquém Ramos
Tematy:
nitrogen assimilation
enzymatic activity
in vivo assay
woody plant
Pokaż więcej
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Powiązania:
https://bibliotekanauki.pl/articles/2027823.pdf  Link otwiera się w nowym oknie
Opis:
Nitrate assimilation in the plant cell is mainly regulated by the enzyme nitrate reductase (EC 1.6.6.1), which catalyzes the nitrate to nitrite reduction. Nitrate reductase activity (NRA) is measured at the major nitrate reduction site, which can be the root or shoot, depending on the species. The in vivo assay has often been used for NRA measurement, and protocol also usually varies with the species. The goals of this study were: (1) to identify the major nitrate reduction site in seedlings of two tree species, Eucalyptus urophylla and Khaya senegalensis, and (2) to optimize the in vivo nitrate reductase assay at the major nitrate reduction site in these species. Healthy seedlings aged 180 and 160 days, respectively, were selected for NRA measurement in fully expanded leaves and main root. After identifying the main nitrate reduction site of each species, the effects of variations in temperature, nitrate concentration and pH in the incubation medium were assessed. The results showed that the leaf and the root are the major nitrate reduction site of Eucalyptus urophylla and Khaya senegalensis, respectively. The optimal conditions for the in vivo assay in the leaf were 35 °C, $KNO_3$ 100 mM, and pH 7.0, whereas for the root they were 30 °C, $KNO_3$ 100 mM, and pH 7.5.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and Characterization of Bacterial Strains with Organic-Degrading Potential for Municipal Wastewater Treatment
Autorzy:
Kulzhanova, Kamshat
Tekebayeva, Zhanar
Temirbekova, Aliya
Bazarhankyzy, Aidana
Temirkhanov, Aslan
Bissenova, Gulmira
Mkilima, Timoth
Sarmurzina, Zinigul
Tematy:
biological method
enzymatic activity
microorganisms
organic compounds
wastewater treatment
Pokaż więcej
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Powiązania:
https://bibliotekanauki.pl/articles/59114196.pdf  Link otwiera się w nowym oknie
Opis:
The increasing volume of wastewater discharged in urban areas poses a significant environmental challenge, particularly due to the potential for organic carbon overload in aquatic ecosystems. This study aimed to identify the bacterial isolates with the potential to mitigate this burden by effectively degrading organic matter and exhibiting antagonistic activity against common aquatic pathogens. Through a screening process, two bacterial strains, Bacillus amyloliquefaciens and Bacillus licheniformis, were isolated based on their high amylolytic, cellulolytic, proteolytic and lipolytic activity indices. In addition, in relation to all effective strains for these activities, Bacillus amyloliquefaciens differed in the cellulolytic index (4.48 ± 0.12), while Bacillus licheniformis had a lipolytic index of 1.73 ± 0.10. Both strains were further characterized by their strong antagonistic activity against Aeromonas, a prevalent pathogen in aquatic environments. These findings suggest that B. amyloliquefaciens and B. licheniformis hold promise as bioremediation agents for wastewater treatment, potentially contributing to the sustainable management of urban wastewater and the protection of aquatic ecosystems.
Dostawca treści:
Biblioteka Nauki
Artykuł

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