Temat: explant - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’
Regeneracja in vitro Citrus limon L. Burm cv. ‘Primofiore’ z eksplantatów liściowych
Autorzy:: Kasprzyk-Pawelec, A.
Pietrusiewicz, J.
Szczuka, E.
Tematy:: in vitro
plant regeneration
leaf explant
explant
callus induction
auxin
cytokinin
Citrus limon
Primofiore cultivar; Pokaż więcej
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Powiązania:: https://bibliotekanauki.pl/articles/11543009.pdf Link otwiera się w nowym oknie
Opis:: In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg·L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon. t
Organogenezę in vitro badano u Citrus limon L. Burm cv. ‘Primofiore’, wykorzystując eksplantaty z liści. Celem badań była optymalizacja warunków do indukcji tkanki kalusowej oraz regeneracji roślin C. limon. Eksplantaty C. limon wykładano na 16 pożywek różnego rodzaju, które dodatkowo uzupełniano kombinacjami roślinnych regulatorów wzrostu, zarówno auksyn, jak i cytokinin, takimi jak 6-benzylaminopuryna (BAP), kwas naftalenooctowy (NAA), kwas 2,4-dichlorofenoksyoctowy (2,4-D) i kinetyna. Regenerację pędową uzyskano, gdy eksplantaty liściowe wykładano na pożywkę Murashige and Tuckera uzupełnioną 3,5 mg·L-1 BAP. Badania histologiczne pozwoliły na przedstawienie początkowej fazy rozwoju eksplantatów z liści w trakcie regeneracji in vitro C. limon.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: In vitro morphogenic events in culture of Lotus corniculatus L. seedling root explants
Autorzy:: Rybczynski, J J
Karolkowska, M.
Kaczmarek, Z.
Mikula, A.
Fiuk, A.
Tematy:: in vitro culture
morphogenic event
Lotus corniculatus
seedling root explant
plant regeneration
cytokinin
callus proliferation
shoot differentiation
root explant; Pokaż więcej
Wydawca:: Polskie Towarzystwo Botaniczne
Powiązania:: https://bibliotekanauki.pl/articles/57212.pdf Link otwiera się w nowym oknie
Opis:: The experiments were carried out on Lotus corniculatus (L.) seedling root explants of the cultivar varieties Skrzeszowicka, Caroll A10 and strain 175. Callus formation and shoot regeneration were the major explant response depended mainly on of the studied genotype and used plant growth regulators (PGRs). Primary cortex of proximal and distal end of explant was the most active tissue for callus proliferation. For shoot primordia differentiation deeper zones of cortex took a part. The process of meristematic centre initiation was not uniform and various level of shoot differentiation events were observed not earlier than 3 weeks of culture. Usually, the shoot primordia regeneration began on proximal rather than distal end of the explant. BAP rather than urea derivatives stimulated shoot proliferation in extended cultures. Increasing of BAP and TDZ concentrations brought about the explant polarity and expansion of the meristematic zones. The explant position in root did not have significant influence on the number of regenerated shoots. The cultures only had better bud formation by TDZ when compared to BAP. BAP stimulated bud formation and development of the shoots from them. Short term of TDZ treatment of explants stimulated meristem formation which developed into buds and shoots. CPPU stimulated callus proliferation and bud formation when explants pretreatment was prolonged from 12 to 36 hrs.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Indirect shoot regeneration from leaf explants of Kalanchoe pinnata
Autorzy:: Andrzejewska, K.
Durska, A.
Kempa, M.
Kopec, P.
Sobusiak, A.
Stec, N.
Trzebny, A.
Tematy:: shoot regeneration
leaf explant
Kalanchoe pinnata
Crassulaceae
micropropagation
histology; Pokaż więcej
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:: https://bibliotekanauki.pl/articles/951233.pdf Link otwiera się w nowym oknie
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis
Autorzy:: Chornobrov, Oksana
Bilous, Svitlana
Wydawca:: Committee on Forestry Sciences and Wood Technology of the Polish Academy of Sciences and the Forest Research Institute in Sękocin Stary
Cytata wydawnicza:: Chornobrov, Oksana and Bilous, Svitlana. "In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis" Folia Forestalia Polonica, vol.63, no.1, 2021, pp.68-73. https://doi.org/10.2478/ffp-2021-0007
Opis:: Plants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more than 90%) of S. truncata plant explants using 0.1% HgCl2 for 7–8 min was established. Optimal conditions for the induction of callus formation in stem node segments of S. truncata plants (rate more than 90% and significant growth) were created on MS (Murashige and Skoog 1962) nutrient medium supplemented with 1.0 mg/l BAP (6-benzylaminopurine) and 0.3 mg/l NAA (1-naphthylacetic acid) under conditions of placement on the nutrient medium and doing a significant number of cuts on the explants. The light intensity of 2.0–3.0 klx, obtained by a callus of dense consistency of dark green pigmentation, when using the thermostat condition without illumination, the callus had loose consistency, dark yellow pigmentation. It is established that the influence of the lighting regime and the composition of the nutrient medium on the frequency of callus formation is statistically significant. The largest number of shoots was obtained on the MS medium with the addition of 2.0 mg/l of BA. At the same times, shoot proliferation and root induction in such numbers were observed on MS culture medium with the addition of 0.5 mg/l BA and 0.5 mg/l kinetin (multiplication factor – 8.8±0.6 per 60-day cultivation cycle).
Dostawca treści:: Repozytorium Centrum Otwartej Nauki

Artykuł

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Skocz do pozycji: 5.

Tytuł:: In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis
Autorzy:: Chornobrov, Oksana
Bilous, Svitlana
Tematy:: Christmas cactus
nutrient medium
explant
callus
shoot proliferation
microclonal propagation; Pokaż więcej
Wydawca:: Instytut Badawczy Leśnictwa
Powiązania:: https://bibliotekanauki.pl/articles/2041281.pdf Link otwiera się w nowym oknie
Opis:: Plants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more than 90%) of S. truncata plant explants using 0.1% HgCl2 for 7–8 min was established. Optimal conditions for the induction of callus formation in stem node segments of S. truncata plants (rate more than 90% and significant growth) were created on MS (Murashige and Skoog 1962) nutrient medium supplemented with 1.0 mg/l BAP (6-benzylaminopurine) and 0.3 mg/l NAA (1-naphthylacetic acid) under conditions of placement on the nutrient medium and doing a significant number of cuts on the explants. The light intensity of 2.0–3.0 klx, obtained by a callus of dense consistency of dark green pigmentation, when using the thermostat condition without illumination, the callus had loose consistency, dark yellow pigmentation. It is established that the influence of the lighting regime and the composition of the nutrient medium on the frequency of callus formation is statistically significant. The largest number of shoots was obtained on the MS medium with the addition of 2.0 mg/l of BA. At the same times, shoot proliferation and root induction in such numbers were observed on MS culture medium with the addition of 0.5 mg/l BA and 0.5 mg/l kinetin (multiplication factor – 8.8±0.6 per 60-day cultivation cycle).
Dostawca treści:: Biblioteka Nauki