Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Pedagogiczna Biblioteka Wojewódzka im. Komisji Edukacji Narodowej w Lublinie Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaPedagogiczna Biblioteka Wojewódzka im. Komisji Edukacji Narodowej w Lublinie
Zmień bibliotekę

Wyszukujesz frazę "mAb" wg kryterium: Temat

  Lista filtrów
Wyrównaj
Wyświetlanie 1-10 z 13
Tytuł:
Production of anti-hTNF monoclonal antibodies using hybridoma technology
Produkcja przeciwciał monoklonalnych anty-hTNF z zastosowaniem metody opartej o komórki hybrydoma
Autorzy:
Mazurek, Agata
Opis:
Czynnik martwicy nowotworu jest cytokiną odgrywającą kluczową role w odpowiedzi układ immunologicznego. Dzięki swoim wszechstronnym funkcjom wpływa praktycznie na każdy typ komórek w organizmie. Pobudza szereg ścieżek sygnalizacyjnych obejmujących m.in. ekspresję genów prozapalnych, stymulację proliferacji i różnicowania komórek, a także aktywację apoptozy lub nekroptozy.Celem pracy było wytworzenie przeciwciał monoklonalnych rozpoznających specyficznie ludzkie białko TNF przy użyciu technologii hybrydoma. Przeprowadzono pozytywną immunizację dwóch myszy stosując jako antygen oczyszczony TNF pozyskany z hodowli komórek ze stabilną ekspresją tego białka. Testem ELISA określono miano przeciwciał w surowicy immunizowanych myszy. Monitorowano poziom immunizacji i po uzyskaniu miana minimum 1:10000, przeprowadzono izolacje mysich splenocytów i kolejno wykonano ich fuzje z nieśmiertelnymi komórkami szpiczaka mnogiego SP2/0-Ag14. W wyniki hybrydyzacji otrzymano 15 pozytywnych klonów, spośród których wybrano 2 o najwyższym powinowactwie w testach ELISA. Wybrane klony poddano dwukrotnym subklonowaniom. W celu uzyskania przeciwciał na większą skalę kolekcjonowano pożywki hodowlane hybrydoma i za pomocą chromatografii powinowactwa oczyszczono immunoglobuliny monoklonalne. Metodą SDS-PAGE określono ich masę cząsteczkową i stopień czystości.
Tumor necrosis factor (TNF) is a cytokine that plays a key role in the immune response. Thanks to its pleiotropic function, TNF affects virtually any type of cell in the body. It stimulates several signalling pathways, among others, expression of proinflammatory genes, stimulation of cell proliferation and differentiation, as well as activation of apoptosis or necroptosis.This study aimed to produce monoclonal antibodies that specifically recognize the human TNF protein using hybridoma technology. Two mice were positively immunized using as antigen purified TNF obtained from cell culture stably expressing this protein. The antibody titer in the serum of the immunized mice was determined by ELISA. The level of immunization was monitored and after obtaining a minimum titer of 1:10000, mouse splenocytes were isolated and sequentially fused with immortal SP2/0-Ag14 multiple myeloma cells. As a result of hybridization, 15 positive clones have been obtained, two of them, with the highest affinity in ELISA, were selected. Selected clones were subcloned twice. In order to obtain antibodies on a larger scale, hybridoma culture media was collected and using affinity chromatography monoclonal immunoglobulins were purified. Using SDS-PAGE method determined their molecular weight and degree of purity.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Inne
Tytuł:
Phase 2B randomized study of nemolizumab in adults with moderate-to-severe atopic dermatitis and severe pruritus
Autorzy:
Piketty, Christophe
Pulka, Grażyna
Poulin, Yves
Pinter, Andreas
Murrell, Dedee F.
Silverberg, Jonathan I.
Alexis, Andrew
Lindsey, Lisa
Ahmad, Faiz
Clucas, Alan
Bouaziz, Jean-David
Wollenberg, Andreas
Opis:
Background: Nemolizumab targets the IL-31 receptor a subunit involved in atopic dermatitis (AD) pathogenesis. Objective: We sought to evaluate a new dosing strategy of nemolizumab in patients with AD. Methods: We performed a 24-week, randomized, double-blind, multicenter study of nemolizumab (10, 30, and 90 mg) subcutaneous injections every 4 weeks versus placebo, with topical corticosteroids in adults with moderate-to-severe AD, severe pruritus, and inadequate control with topical treatment (n 5 226). The Eczema Area and Severity Index (EASI), the peak pruritus (PP) numeric rating scale (NRS), and the Investigator’s Global Assessment (IGA) were assessed. Standard safety assessments were performed. Results: Nemolizumab improved EASI, IGA, and/or NRS-itch scores, with the 30-mg dose being most effective. Nemolizumab (30 mg) reduced EASI scores versus placebo at week 24 (268.8% vs 252.1%, P 5 .016); significant differences were observed by week 8 (P <_ .01). With significant improvement (P 5 .028) as early as week 4, IGA 0/1 rates were higher for 30 mg of nemolizumab versus placebo at week 16 (33.3% vs 12.3%, P 5 .008) but not week 24 because of an increased placebo/topical corticosteroid effect (36.8% vs 21.1%, P 5 .06). PP-NRS scores were improved for 30 mg of nemolizumab versus placebo at week 16 (268.6% vs 234.3%, P <.0001) and week 24 (267.3% vs 235.8%, P <.0001), with a difference by week 1 (P < .001). NRS response rates (>_4-point decrease) were greater for 30 mg of nemolizumab versus placebo at week 16 (P <_ .001) and week 24 (P <_ .01). Nemolizumab was safe and well tolerated. The most common adverse events were nasopharyngitis and upper respiratory tract infection. Conclusions: Nemolizumab resulted in rapid and sustained improvements in cutaneous signs of inflammation and pruritus in patients with AD, with maximal efficacy observed at 30 mg. Nemolizumab had an acceptable safety profile. (J Allergy Clin Immunol 2020;145:173-82.)
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Tytuł:
Kierunki i stan opracowania międzynarodowego programu "Człowiek a Biosfera" (CzAB)
Directions and present state of work on the international programme "Man and Biosphere" (MAB)
Kierunki i stan opracowania programu CzAB
Autorzy:
Petrusewicz, Kazimierz
Współwytwórcy:
Polska Akademia Nauk. Komitet Ekologiczny
Wydawca:
Państwowe Wydawnictwo Naukowe
Powiązania:
Wiadomości Ekologiczne
Opis:
A summary of the project for the MAB programme presented at the XVI session of UNESCO. Emphasis is laid on the many-sided, logical and highly accurate el1aboration of this project. Critical consideration of the preference for studies on natural ecosystems over ecosystems changed and created by man. The reason for this preference is based on the idea: a knowledge of the functioning of natural ecosystems will permit of (1) understanding the functioning of transformed ecosystems and (2) foreseeing side-effects, the degree of stability etc. of transformed ecosystems. Thus the ecosystems created by man should be formed in the image and likeness of natural ecosystems. In polemizing with this idea the following assumptions are put forward: (1) biocenoses of cultivated fields are more productive than natural ecosystems: they are essential and the area they occupy will increase: (2) a knowledge of their ecology and functioning will be obtained more rapidly by studying them directly than by comparing their life with the life of natural ecosystems: (3) the biocenoses of cultivated fields are frequently ecosystems so altered (plant monocultures) that they may often be subject to other laws and ecological regularities than natural ones: in such cases even a full knowledge of the organization (structures and functions) of natural ecosystems may be insufficient for obtaining a knowledge of the ecology of culttvated field biocenoses.
Streszczenie w języku angielskim
Abstract in English
Strony 17-28 ; 24 cm
Pages 17-28 ; 24 cm
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Tytuł:
Wiadomości Hydrobiologiczne
Międzynarodowe sympozjum UNESCO/MAB-5 na temat "Wpływ użytkowania terenu na ekosystemy wodne - wykorzystanie informacji naukowej" (Tuluza, 21-25 IV 1986 r.)
Autorzy:
Hillbricht-Ilkowska, Anna (1932–2021)
Współwytwórcy:
Polska Akademia Nauk. Komitet Ekologii
Wydawca:
Państwowe Wydawnictwo Naukowe
Powiązania:
Wiadomości Ekologiczne
Opis:
Pages 96-98 ; 24 cm
Strony 96-98 ; 24 cm
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Tytuł:
Efficient Wireless Sensor Network for Radiation Detection in Nuclear Sites
Autorzy:
Hashima, Sherief
Mahmoud, Imbab
Tematy:
wireless sensor networks (WSNs)
radiation detection
multi-armed bandit (MAB)
Thomson sampling
network lifetime
Pokaż więcej
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/1844636.pdf  Link otwiera się w nowym oknie
Opis:
Due to the severe damages of nuclear accidents, there is still an urgent need to develop efficient radiation detection wireless sensor networks (RDWSNs) that precisely monitor irregular radioactivity. It should take actions that mitigate the severe costs of accidental radiation leakage, especially around nuclear sites that are the primary sources of electric power and many health and industrial applications. Recently, leveraging machine learning (ML) algorithms to RDWSNs is a promising solution due to its several pros, such as online learning and self-decision making. This paper addresses novel and efficient ML-based RDWSNs that utilize millimeter waves (mmWaves) to meet future network requirements. Specifically, we leverage an online learning multi-armed bandit (MAB) algorithm called Thomson sampling (TS) to a 5G enabled RDWSN to efficiently forward the measured radiation levels of the distributed radiation sensors within the monitoring area. The utilized sensor nodes are lightweight smart radiation sensors that are mounted on mobile devices and measure radiation levels using software applications installed in these mobiles. Moreover, a battery aware TS (BATS) algorithm is proposed to efficiently forward the sensed radiation levels to the fusion decision center. BA-TS reflects the remaining battery of each mobile device to prolong the network lifetime. Simulation results ensure the proposed BA-TS algorithm’s efficiency regards throughput and network lifetime over TS and exhaustive search method.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zadania i możliwości ekologii w zakresie problematyki "Człowiek i środowisko"
Tasks and opportunities for ecology in relation to "Man and Biosphere" problems
Autorzy:
Trojan, Przemysław
Współwytwórcy:
Polska Akademia Nauk. Komitet Ekologiczny
Wydawca:
Państwowe Wydawnictwo Naukowe
Powiązania:
Prończuk J., Pawlat H. 1971 - Przewodnik do ćwiczeń terenowych z ekologii roślin -Warszawa, 171 pp.
Armand D. L. - 1967 - Nekotorye zadaci i metody fiziki landsafta - Geofizika Landsafta: 7-24.
Molga M. 1966 - Meteorologia rolnicza - Warszawa 571 pp.
Wiadomości Ekologiczne
Petrusewicz K. 1966 - Dynamics organisation and ecological structure of population - Ekol. Pol. A, 14: 413-436.
Wolak J. 1969 - Industrioklimaks, nowe pojęcie w teorii sukcesii - Ekol. Pol. B, 15: 41-44.
Wilusz Z. 1958 - Wpływ zadrzewienia ochronnego na gospodarkę wodną i planowanie przyległych terenów - Ekol. Pol. A, 6: 1-52.
Karcagin A. A., Lukiceva A. N., Saburov D. T., 1971 – Teoresticeskie voprosy fitoindykacii -Leningrad, 214 pp.
Pyżuk M., Wolański N. 1968 - Niektóre właściwości fizjologiczne dzieci i młodzieży jako wynik adaptacji do różnych warunków środowiskowych - Prace Mat. Nauk. IMD, 11: 129-151.
Koehler W. 1968 - Biologiczne metody ochrony lasu - Warszawa 199 pp.
Azzi G. 1956 - Agricultural ecology - London, 340 pp.
Michajłow W. 1969 - Człowiek a środowisko - LOP, 16 pp.
Schwerdtfeger F. 1963 - Autokologie - Hamburg, Berlin, 461 pp.
Zacharow P. S. 1971 - Erozja pocv i mery borby s nej - Moskva, 191 pp.
Kaczmarek W. 1963 - An analysis of interspecific competition and communities of the soil macrofauna of same habitats in the Kampinos National Park - Ekol. Pol. A, 11: 421-483.
Obmiński Z. 1970 - Ekologia a węzłowe problemy współczesnego leśnictwa - Wiad. ekol. 16: 107-116.
Wolański. N., Pyżuk M. 1968 - Niektóre właściwości mieszkańców zapylonego miasta przemysłowego (Katowice) na tle mieszkańców Warszawy - Prace Mat. Nauk. IMB, 11: 105-127.
Opis:
In respect of agrocenoses the ecological method constitutes a basic expert opinion preceding plans for setting up and improving agricultural land. This method is taught in agricultural colleges. The problem of studies on the habitat requirements of cultivated plants has, however, been neglected in Poland up to the present. Conservation of cultivated field biocenoses, both in relation to hatbitat conditions and pest protection of crops, has a long tradition in Poland in connection with research on windbreaks. One of the aims of such studies should be to define conditions for the change over in agrocenoses to the intervention method of chemical protection of cultivated plants. The ecological method has been widely used in forest ecosystems as a basis for classifying biotopes, and for defining on this basis optimum plant associations for forest production. The biological method which has been successfully tried out in Poland permits of improving the staibility of forest ecosystems and protecting them from mass attacks of pests. In aquatic ecosystems the following ecological engineering problems are of prime importane: biological protection of ecosystems, in particular streams and rivers in industrialized areas, formation of new ecosystems in associations subject to influx of heated waste water and limitation of euthrophization processes in lakes. They are, however, a large number of deficiences in ecological studies in Poland due to the specific attitude of research workers to basic eco1ogical questions. Ecologists are chiefly concerned with the biological component of the ecosystem, often to the complete exclusion of research on the environment. The current state of research on the homeostasis of ecosystems is unsatisfactory; this theory cannot constitute a basis for formulating tasks to be carried out under the programme. Ecological methods do not as yet include indicator methods which could be used for general evaluation of the state of the environment and ecosystem, and cooperation with sciences concerned with the environment is insufficient. Formulation of tasks to be carried out by ecological sciences in connection with the „Man and Biosphere'' programme should be preceded by discussion of theoretical and methodological premises essential to the undertaking of a research programme new to ecology.
Streszczenie w języku angielskim
Bibliografia na stronie 291
Abstract in English
Bibliographical references (page 291)
Pages 282-293 ; 24 cm
Planning of research programmes in different fields of science in connection with the ''Man and Biosphere'' programme is being influenced by the pressure of public opinion demanding solution of problems directly involving the preservation of man's environment. As far as the biological sciences are concerned research work on preservation of the biosphere necessitates, in the majority of cases, both a radical change in thought and in approaoh to scientific problems. Ecological sciences should play an important part in studies on the „Man and Biosphere'' programme, since both the research unit - the ecosystem ecological theory, in particular the concept of homeostasis and the problems of ecological regulation and compensation deriving from it, and also the quantitative methods employed, cover all basie and methodological scientific questions arising from the programme. Ecological research problems involved in the ''Man and Biosphere'' programme costitute two groups of subjects: recording of anthropogenic distutibances in ecosystems and ecological engineering of ecosystems. The main effort in this connection should be directed at elaboration of balances and expert opinions, based on them, of changes taking place in the environment or whole ecosystems. Recording of anthropogenic disturbance in ecosystems covers five groups of subjects: 1) the effect of man-made pollution of the environment on matter and energy economy in ecosystems; 2) listing of all man-made disturbances in ecosystems; 3) accumulation and biodegradation of toxic substances in ecosystems; 4) studies on bioindicators of environment pollution and state of ecosystems; 5) studies on man's reaction to changes taking place in the environment. Implementation of this programme of studies in this field should provide ecology with firm bases for formulating expert opinions and forecasting changes in the environment, based on the known initial state of the ecosystem, probable intoxication of the environment and known ecological process which will be set in motion as the result of planned economic measures. Ecological engineering of ecosystems postulated as ecology of the future is today developing in three directions; research work covers the following: 1) adaptation of biological systems to changed environment conditions; 2) conservation of ecosystems; 3) formation of new ecosystems. The purpose of such studies is to elaborate scientific expert opinions in order to design ecosystems in which biological components remain in a state of balance with the biosphere.
Strony 282-293 ; 24 cm
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Wyświetlanie 1-10 z 13

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies