Temat: microsatellite DNA - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: Are we narrowing genetic variability in seed orchards? An attempt to answer, based on the analysis of microsatellite DNA of grafts growing in Scots pine (Pinus sylvestris L.) seed orchard in the Forest District Susz
Autorzy:: Przybylski, Paweł
Wydawca:: Instytut Badawczy Leśnictwa (Forest Research Institute), Sekocin Stary, Poland
Opis:: Scots pine (Pinus sylvestris L.) is the most common species in Poland’s forest stands. The mode of pine stands renovation requires that silviculture practitioners have continuous access to seed banks. Orchard-grown seeds are predicted to constitute an increasingly larger part of the average demand for pine seeds in Poland. Seed orchards, due to a limited number of maternal trees as well as the irregularity of their blooming and pollination, enhance the risk of genetic diversity reduction in planted forest stands. This is of particular importance in the context of dynamic climate change. Markers based on microsatellite DNA fragments are effective tools for monitoring genetic variability. In the present study, three different microsatellite DNA fragments were used: SPAC 12.5, SPAG 7.14 and SPAC 11.4. The main objective of this research was to study genetic variability in one of the biggest seed orchards in Poland, located in the Forest District Susz. The obtained results indicated heterozygosity loss within the orchard, proving the existence of specimen selection effects on genetic variability. Hence, it seems quite important to take account of molecular genetic variability of maternal trees in future breeding strategies.
Przemysław Szmit
Dostawca treści:: Repozytorium Centrum Otwartej Nauki

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Skocz do pozycji: 2.

Tytuł:: Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
Autorzy:: Kaczmarczyk, D.
Kohlmann, K.
Kersten, P.
Luczynski, M.
Tematy:: izolacja DNA
DNA mikrosatelitarne
amplifikacja PCR
DNA extraction
microsatellite DNA
PCR amplification; Pokaż więcej
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Powiązania:: https://bibliotekanauki.pl/articles/363088.pdf Link otwiera się w nowym oknie
Opis:: American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Genetic identification of black caviar based on microsatellite DNA analysis
Autorzy:: Fopp-Bayat, D.
Tematy:: identyfikacja genetyczna
czarny kawior
DNA mikrosatelitarne
genetic identification
black caviar
microsatellite DNA; Pokaż więcej
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Powiązania:: https://bibliotekanauki.pl/articles/363094.pdf Link otwiera się w nowym oknie
Opis:: Sturgeons (Acipenseridae) are producers of black caviar, which is sold world-wide. Black caviar differs widely in quality, availability, price and taste and for these reasons it is often the subject of commercial fraud. Identification of sturgeon species is frequently based on molecular methods such as analysis of nuclear DNA and mitochondrial DNA. In this study two fragments of microsatellite DNA: Afu-39 and Afu-68 were analyzed. In caviar samples, four alleles were observed in locus Afu-39, and six alleles in locus Afu-68. This paper describes the use of microsatellite DNA markers for species identification of black caviar.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: New multiplex PCR assays for estimating genetic diversity in rainbow trout (Oncorhynchus mykiss) by polymorphism of microsatellite DNA
Autorzy:: Kaczmarczyk, D.
Kaczor, A.
Tematy:: microsatellite DNA
multiplex PCR
population genetics
rainbow trout
DNA mikrosatelitarne
genetyka populacyjna
pstrąg tęczowy; Pokaż więcej
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Powiązania:: https://bibliotekanauki.pl/articles/363262.pdf Link otwiera się w nowym oknie
Opis:: Multiplex PCR is a useful technique for estimating genetic diversity. This paper presents 3 new sets of primer pairs for effectively amplifying 10 microsatellite DNA loci from rainbow trout (Oncorhynchus mykiss). Unlike other sets of primer pairs that have been developed for amplifying rainbow trout microsatellite loci, ours do not require the hot-start PCR technique. In the paper, we describe the steps taken to choose the loci for each multiplex assay and to verify the genotyping results. We provide the compositions of the PCR mixture and the characteristics of the PCR thermal profile recommended for amplification.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Genetic variation of silver fir progeny from Tisovik Reserve population determined via microsatellite and isozyme markers
Autorzy:: Pawlaczyk, Ewa Maria
Bączkiewicz, Alina
Bobowicz, Maria Anna
Buczkowska, Katarzyna
Wydawca:: Forest Research Institute in Poland and Polish Academy of Sciences
Opis:: Progeny from 19 family lines of silver fir (Abies alba Mill.) from a small, native and isolated population from the Tisovik Reserve (Belarusian part of Białowieża Primeval Forest) growing in an experimental plot near Hajnówka (Polish part of Białowieża Primeval Forest) were analysed in terms of 4 nuclear microsatellite DNA loci and 9 isozyme systems with 14 loci. The aim of this study was to determine the genetic variation within and between progeny lines. Analysis of isozyme loci showed that all progeny lines, except the progeny lines T6 and T16, were characterised by an excess of heterozygotes and 20% of the detected variation occurred between progeny. Progeny formed two groups. Microsatellite loci showed that 6 progeny lines demonstrated an excess of heterozygotes and 12 an excess of homozygotes. On an average, the population was in the Hardy–Weinberg equilibrium. Analysis of molecular variance (ANOVA) showed that 14% of the detected variation occurred between offspring and the remaining 86% within progeny lines. The most distinct progeny line was T1, where the highest number of alleles per locus was detected. Generally, progeny of Tisovik is characterised by high level of differentiation as the offspring of isolated population that have limited number of individuals to crossing (only 20). In some progeny line, the private alleles that are detected may be the result of pollination from Polish part of Białowieża Forest where in 1920s and 1930s of XX century had planted the seedling of silver fir of unknown origin. The substructuring of population is observed, and the detected deficiency of heterozygotes may be ostensible as a result of the Wahlund effect. Such pattern of genetic structure could also be an effect of harsh environmental conditions exerting selection pressure and modifying the genetic composition of this population.
Dostawca treści:: Repozytorium Centrum Otwartej Nauki

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Skocz do pozycji: 6.

Tytuł:: Microsatellite DNA polymorphism in sturgeon species and their hybrids reared in Polish aquaculture farms
Autorzy:: Fopp-Bayat, D.
Luczynski, M.
Tematy:: jesiotr
identyfikacja gatunkowa
DNA mikrosatelitarne
hybrydy
polimorfizm
hybrids
microsatellite DNA
polymorphism
PCR
species identification
sturgeons; Pokaż więcej
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Powiązania:: https://bibliotekanauki.pl/articles/363194.pdf Link otwiera się w nowym oknie
Opis:: Highly variable microsatellite DNA loci show high levels of variation per locus and provide molecular markers for species and for populations of small effective size. In this study we applied microsatellite markers to identify specimens belonging to several sturgeon species and their interspecific hybrids. Nine microsatellite loci (Afu-19, Afu-22, Afu-34, Afu-39, Afu-54, Afu-57, Afu-58, Afu-68, Afu-69) were analysed for five species (beluga, Siberian sturgeon, Russian sturgeon, sterlet and paddlefish) and for their three interspecific hybrids: Siberian sturgeon x Russian sturgeon, beluga x sterlet (called bester) and for beluga x bester. Certain alleles at five loci: Afu-22, Afu-39, Afu-54, Afu-57 and Afu-68 were diagnostic for the examined sturgeon species and their interspecific hybrids.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Variability of Scots pine (Pinus sylvestris L.) called Tabórz pine (Forest District Miłomłyn) expressed in analysis of morphology of needle traits and polymorphism of microsatellite DNA
Zmienność taborskiej sosny zwyczajnej (Pinus sylvestris L.) z Nadleśnictwa Miłomłyn wyrażona w analizie cech morfologii igieł oraz polimorfizmie mikrosatelitarnego DNA
Autorzy:: Lesiczka, Paulina
Pawlaczyk, Ewa M.
Urbaniak, Lech
Łabiszak, Bartosz
Wydawca:: Instytut Badawczy Leśnictwa (Forest Research Institute), Sękocin Stary, Poland
Opis:: Scots pine (Pinus sylvestris L.) is one of the most widespread forest trees in the world, ranging from southern Mediterranean mountains to eastern Siberia. 30 trees of Scots pine from the Miłomłyn Forest District were analyzed in terms of 7 nuclear and 8 chloroplast microsatellite DNA loci as well as 7 morphological needle traits. Nuclear microsatellites (Simple Sequence Repeats) have proved to be useful in studying phylogeographic and gene flow patterns in conifers and are being used to infer the demographic history of tree species. A population’s genetic diversity, in fact, represents an important criterion which could be applied in planning future forest management and breeding. Additionally, many elements of Scots pine morphology, including needle traits, are subject to environmental modification. However, the adaptability of those traits remains, to some extent, under genetic control. The trees we investigated here are called Tabórz pine located in the northeast of Poland. This population is described as one of the best in Poland and even in Europe regarding bio mass production, ductility and wood quality. The aim of the study was to determine variation and genetic structure within this population. An analysis of phenotypic differentiation showed that the traits which differed the most between individuals were needle length, the number of serrations per 2 mm of needle length along the right edge and the number of stomatal rows on the convex side of the needles. In Tabórz pine, we detected 30 different genotypes with 87 alleles as well as 28 haplotypes with 30 alleles. We also revealed a slight excess of homozygotes, but the population is still in Hardy-Weinberg equilibrium. The Scots pine population from Tabórz furthermore showed a higher level of genetic diversity compared to stands from other Polish and European regions. This diversity may be the main factor impacting on the population’s wood quality and its breeding value.
Dostawca treści:: Repozytorium Centrum Otwartej Nauki

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Skocz do pozycji: 8.

Tytuł:: Czy na plantacjach nasiennych zawężamy zmienność genetyczną? Próba odpowiedzi na podstawie analiz mikrosatelitarnego DNA szczepów rosnących na plantacji nasiennej sosny zwyczajnej (Pinus sylvestris L.) z Nadleśnictwa Susz
Are we narrowing genetic variability in seed orchards? An attempt to answer, based on the analysis of microsatellite DNA of grafts growing in Scots pine (Pinus sylvestris L.) seed orchard in the Forest District Susz
Autorzy:: Przybylski, P.
Tematy:: lesnictwo
hodowla lasu
plantacje nasienne
sosna zwyczajna
Pinus sylvestris
zmiennosc genetyczna
DNA mikrosatelitarny
analiza DNA
microsatellite DNA
tree breeding
seed orchard; Pokaż więcej
Wydawca:: Instytut Badawczy Leśnictwa
Powiązania:: https://bibliotekanauki.pl/articles/1311426.pdf Link otwiera się w nowym oknie
Opis:: Scots pine (Pinus sylvestris L.) is the most common species in Poland’s forest stands. The mode of pine stands renovation requires that silviculture practitioners have continuous access to seed banks. Orchard-grown seeds are predicted to constitute an increasingly larger part of the average demand for pine seeds in Poland. Seed orchards, due to a limited number of maternal trees as well as the irregularity of their blooming and pollination, enhance the risk of genetic diversity reduction in planted forest stands. This is of particular importance in the context of dynamic climate change. Markers based on microsatellite DNA fragments are effective tools for monitoring genetic variability. In the present study, three different microsatellite DNA fragments were used: SPAC 12.5, SPAG 7.14 and SPAC 11.4. The main objective of this research was to study genetic variability in one of the biggest seed orchards in Poland, located in the Forest District Susz. The obtained results indicated heterozygosity loss within the orchard, proving the existence of specimen selection effects on genetic variability. Hence, it seems quite important to take account of molecular genetic variability of maternal trees in future breeding strategies.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Zmienność taborskiej sosny zwyczajnej (Pinus sylvestris L.) z Nadleśnictwa Miłomłyn wyrażona w analizie cech morfologii igieł oraz polimorfizmie mikrosatelitarnego DNA
Variability of Scots pine (Pinus sylvestris L.) called Taborz pine (Forest District Milomlyn) expressed in analysis of morphology of needle traits and polymorphism of microsatellite DNA
Autorzy:: Lesiczka, P.
Pawlaczyk, E.M.
Łabiszak, B.
Urbaniak, L.
Tematy:: lesnictwo
Nadlesnictwo Milomlyn
drzewa lesne
sosna zwyczajna
Pinus sylvestris
zmiennosc
sosna taborska
igly sosny
cechy morfologiczne
DNA mikrosatelitarny
polimorfizm genetyczny
markery molekularne
genom jadrowy
genom chloroplastowy
Scots pine from Tabórz
needle morphology
nuclear (nrSSR) and chloroplast (cpSSR) microsatellite DNA
genetic
variation; Pokaż więcej
Wydawca:: Instytut Badawczy Leśnictwa
Powiązania:: https://bibliotekanauki.pl/articles/1292493.pdf Link otwiera się w nowym oknie
Opis:: Scots pine (Pinus sylvestris L.) is one of the most widespread forest trees in the world, ranging from southern Mediterranean mountains to eastern Siberia. 30 trees of Scots pine from the Miłomłyn Forest District were analyzed in terms of 7 nuclear and 8 chloroplast microsatellite DNA loci as well as 7 morphological needle traits. Nuclear microsatellites (Simple Sequence Repeats) have proved to be useful in studying phylogeographic and gene flow patterns in conifers and are being used to infer the demographic history of tree species. A population’s genetic diversity, in fact, represents an important criterion which could be applied in planning future forest management and breeding. Additionally, many elements of Scots pine morphology, including needle traits, are subject to environmental modification. However, the adaptability of those traits remains, to some extent, under genetic control. The trees we investigated here are called Tabórz pine located in the northeast of Poland. This population is described as one of the best in Poland and even in Europe regarding bio mass production, ductility and wood quality. The aim of the study was to determine variation and genetic structure within this population. An analysis of phenotypic differentiation showed that the traits which differed the most between individuals were needle length, the number of serrations per 2 mm of needle length along the right edge and the number of stomatal rows on the convex side of the needles. In Tabórz pine, we detected 30 different genotypes with 87 alleles as well as 28 haplotypes with 30 alleles. We also revealed a slight excess of homozygotes, but the population is still in Hardy-Weinberg equilibrium. The Scots pine population from Tabórz furthermore showed a higher level of genetic diversity compared to stands from other Polish and European regions. This diversity may be the main factor impacting on the population’s wood quality and its breeding value.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Tracing the genetic origin of brown trout (Salmo trutta) re-colonizing the Ecker reservoir in the Hartz National Park, Germany
Autorzy:: Kohlmann, K.
Wüstemann, O.
Tematy:: pstrąg potokowy
Salmo trutta
mikrosatelitarne DNA
DNA
PCR
brown trout
microsatellite; Pokaż więcej
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Powiązania:: https://bibliotekanauki.pl/articles/363212.pdf Link otwiera się w nowym oknie
Opis:: The Ecker reservoir and its main tributary had been free of brown trout (Salmo trutta) for several decades due to cumulative effects of natural and anthropogenic acidification. However, after the decline of emissions in the 1990s and the resulting rise of water pH to suitable for brown trout values, the species began to recolonize its original habitats. In the main tributary first brown trout individuals were caught in 2008 and in the reservoir in later years as well. Stocking could be excluded in both areas. Therefore, the present study was aimed to trace the genetic origin of these brown trout by genotyping eight microsatellite loci in samples collected in the reservoir, its main tributary, potential refugia and - for comparison - from two areas downstream of the dam being physically isolated for about 70 years. Genetic variability within populations (average number of alleles per locus), genetic differentiation between populations (FST values and genetic distances), occurrence of certain alleles and results of assignment tests indicated that the Ecker reservoir was re-colonized from two sources: the Große Peseke, a small direct inflow into the reservoir, and the Fuhler Lohnsbach, a parallel flowing brook connected to the reservoir by a pipe. Genetic data also supported recolonization of the main tributary from the reservoir but not in the opposite direction. Moreover, bottleneck effects were evident in brown trout populations upstream of the dam compared to the two populations downstream of the dam.
Dostawca treści:: Biblioteka Nauki

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