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Wyświetlanie 1-10 z 62
Tytuł:
Ocena zawartości patuliny w zagęszczonym soku jabłkowym z uwzględnieniem etapów procesu produkcyjnego
Assessment of patulin content in apple juice concentrate in regard to production process stages
Autorzy:
Sękul, J.
Worobiej, E.
Bryła, M.
Tematy:
mikotoksyny
mycotoxins
Pokaż więcej
Wydawca:
Centralny Ośrodek Badawczo-Rozwojowy Aparatury Badawczej i Dydaktycznej, COBRABiD
Powiązania:
https://bibliotekanauki.pl/articles/270518.pdf  Link otwiera się w nowym oknie
Opis:
Celem pracy była ocena jakości zagęszczonego soku jabłkowego pod względem zawartości patuliny oraz próba zbadania zmian zawartości tej mikotoksyny w siedmiu głównych etapach procesu produkcji w wybranym zakładzie przetwórczym w środkowym okresie kampanii. Przedmiotem analizy były pobrane w zakładzie przetwórczym próbki: surowca, półproduktów i produktu końcowego tj. zagęszczonego soku jabłkowego. Zawartość patuliny oznaczono metodą wysokosprawnej chromatografii cieczowej. Otrzymane wyniki odniesiono do aktualnych regulacji prawnych w zakresie najwyższych dopuszczalnych zawartości mikotoksyny w produktach spożywczych. Granice wykrywalności i oznaczalności metody dla produktów stałych wynosiły odpowiednio 3,2 i 9,7 |mikro/g/kg, zaś dla produktów płynnych 1,5 i 4,6 |mikro/g/kg. Zawartość patuliny w próbkach płynnych, w tym koncentratu nie przekroczyła 10 |mikro/g/kg. Najwyższą zawartość patuliny stwierdzono w jabłkach pobranych w jednym z terminów w listopadzie ok. 49 |mikro/g/kg.
The aim of this work was to assess apple juice concentrates quality in regard to the patulin content as well as an attempt to investigate the mycotoxin content changes at seven main processing stages in a selected production plant in the middle of a campaign. An object of the analysis were raw material (apples), semi-products, and final product (apple juice concentrate) samples taken in the plant. Patulin content was determined by means of high-performance liquid chromatography. Results obtained were compared with statutory regulations as to the maximum acceptable patulin concentrations in foodstuffs. Limits of detection and quantitation for the method developed were 3,2 and 9,7 |micro/g/kg and 1,5 and 4,6 |micro/g/kg for solid and liquid samples, respectively. Patulin content in liquid samples did not exceed 10 |micro/g/kg. The highest patulin content was found in apples taken in one of the batches from November - approx. 49 |micro/g/kg.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro evaluation of zearalenone toxicity by comet assay
Autorzy:
Harcarova, M.
Conkova, E.
Kolenicova, S.
Holeckova, B.
Proskovcova, M.
Tematy:
mycotoxins
toxicity
comet assay
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Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Powiązania:
https://bibliotekanauki.pl/articles/16539336.pdf  Link otwiera się w nowym oknie
Opis:
The aim of this study was to reveal the potentially genotoxic effect of zearalenone on bovine lymphocytes by comet assay in vitro. The bovine lymphocytes were exposed to various zearalenone concetrations (50; 10; 2; 0.4 and 0.08 ppm). The viability and DNA damage of lymphocytes was monitored after 2 h, 24 h, 48 h and 72 h. After 2 hours of zearalenone exposure, statistically significant DNA damage occurred at all tested concentrations of 0.08 ppm (12.2±1.25; p<0.05), 0.4 ppm (12.7±0.88; p<0.01), 2 ppm (12.0±0.51; p<0.01), 10 ppm (11.2±0.47; p<0.01) and at 50 ppm (14.2±0 61; p<0.001). Significantly greater DNA damage was also found after 24 h, 48 h and 72 h. The obtained results showed that zearalenone may induce DNA damage of the bovine lymphocytes.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of fungicide application on Wheat Head Blight, occurrence of Fusarium spp. and mycotoxin production.
Autorzy:
Baturo-Cieśniewska, A.
Lukanowski, A.
Kolenda, M.
Tematy:
Fusarium
mycotoxins
fungicides
azoxystrobin
metconazole
Pokaż więcej
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Powiązania:
https://bibliotekanauki.pl/articles/2199596.pdf  Link otwiera się w nowym oknie
Opis:
The aim of the study was to determine if azoxystrobin and metconazole used for the control of wheat FHB at half, full, and quarter more the recommended dose rate may affect in differentiated way on the occurrence of Fusarium spp. and their ability to mycotoxin production in harvested grain, in wheat ears artificially inoculated with two DON-producing isolates of F. culmorum. Macroscopic evaluation showed high incidence of fusariosis. Plant health in the plots where the heads were artificially inoculated and fungicide was not applied was similar to the protected ones. Only increasing the dose metconazole resulted in a stronger reduction of fusariosis. The advantageous effect of azoxystrobin was not observed. Mycological analysis of harvested grain showed the presence of a number of F. culmorum, but from samples sprayed with metconazole it was isolated in smaller quantities. Also F. avenaceum, F. graminearum, F. poae and F. tricinctum were isolated. Molecular analysis showed the presence of F. culmorum in all samples of harvested grain. Also genes from Tri cluster were identified, involved in the synthesis of type-A and type-B trichothecenes - especially DON and 3Ac-DON. Chromatography revealed the presence of small quantities of mycotoxins. In all samples DON and 3Ac-DON were predominant. In general, F. culmorum isolate, which caused weaker symptoms of FHB and was less numerously isolated from grain that the other one, produced smaller amounts of mycotoxins. Samples protected with azoxystrobin contain the largest quantities of DON. Effect of different doses of fungicides on the number of mycotoxins was not clearly established...
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of fungicide application on Wheat Head Blight, occurrence of Fusarium spp. and mycotoxin production.
Autorzy:
Baturo-Cieśniewska, A.
Lukanowski, A.
Kolenda, M.
Tematy:
Fusarium
mycotoxins
fungicides
azoxystrobin
metconazole
Pokaż więcej
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Powiązania:
https://bibliotekanauki.pl/articles/55928391.pdf  Link otwiera się w nowym oknie
Opis:
The aim of the study was to determine if azoxystrobin and metconazole used for the control of wheat FHB at half, full, and quarter more the recommended dose rate may affect in differentiated way on the occurrence of Fusarium spp. and their ability to mycotoxin production in harvested grain, in wheat ears artificially inoculated with two DON-producing isolates of F. culmorum. Macroscopic evaluation showed high incidence of fusariosis. Plant health in the plots where the heads were artificially inoculated and fungicide was not applied was similar to the protected ones. Only increasing the dose metconazole resulted in a stronger reduction of fusariosis. The advantageous effect of azoxystrobin was not observed. Mycological analysis of harvested grain showed the presence of a number of F. culmorum, but from samples sprayed with metconazole it was isolated in smaller quantities. Also F. avenaceum, F. graminearum, F. poae and F. tricinctum were isolated. Molecular analysis showed the presence of F. culmorum in all samples of harvested grain. Also genes from Tri cluster were identified, involved in the synthesis of type-A and type-B trichothecenes - especially DON and 3Ac-DON. Chromatography revealed the presence of small quantities of mycotoxins. In all samples DON and 3Ac-DON were predominant. In general, F. culmorum isolate, which caused weaker symptoms of FHB and was less numerously isolated from grain that the other one, produced smaller amounts of mycotoxins. Samples protected with azoxystrobin contain the largest quantities of DON. Effect of different doses of fungicides on the number of mycotoxins was not clearly established...
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Long-Term Storage on Mycobiota of Barley Grain and Malt
Autorzy:
Kacániová, Miroslava
Krajcovic, Tomáš
Dráb, Štefan
Kowalczewski, Przemysław Łukasz
Felšöciová, Sona
Wydawca:
MDPI
Cytata wydawnicza:
Felšöciová S, Kowalczewski PŁ, Krajčovič T, Dráb Š, Kačániová M. Effect of Long-Term Storage on Mycobiota of Barley Grain and Malt. Plants. 2021; 10(8):1655. https://doi.org/10.3390/plants10081655 ; https://www.mdpi.com/2223-7747/10/8/1655
Opis:
Contamination of malting barley grain and malt with micromycetes sampled at various periods post-harvest (3rd, 6th, and 9th month of storage) and types of storage (storage silo and floor warehouse) was investigated. Each of these barley grain samples was malted. This article reports on the changes in the fungal microbiome composition and their overall count in barley grain and malt. From the surface-disinfected barley grain samples collected immediately after harvest, there were eight genera isolated, with a predominance of Alternaria. A small increase of isolated microfungi was detected in barley stored in silo for 3 and 6 months (from 142 isolates to 149) and decreased below the number of isolates in barley before storage (133 isolates). Fungal count during storage gradually decreased up to 9 month in barley stored in floor warehouse (from 142 isolates to 84). The initial total count of microscopic fungi in malt before storage was the highest (112 isolates) with 7 genera detected, compared to malts prepared from barley stored for longer time (54 isolates, 7 genera, 9th month of storage). Alternaria was the most abundant and frequent genus. Quantitative representation of the filamentous microscopic fungi was lower compared to yeasts especially in barley and malt prepared from barley stored at third month of storage in both type of storage. Yeasts were identified from all grain samples and malt samples with mass spectrometry. Most attention was given to the widely distributed fungus Penicillium, 79% of strains produced at least one mycotoxin detected under in vitro assays using the TLC method (97% of them produced griseofulvin, 94% CPA, 79% patulin, 14% roquefortin C, and penitrem A was produced by two screening strains under laboratory conditions). It is therefore important to monitor the microflora throughout the production cycle of “barley to beer”.
Dostawca treści:
Repozytorium Centrum Otwartej Nauki
Artykuł
Tytuł:
Application of probiotics in the xenobiotic detoxification therapy
Autorzy:
Urban, P.
Kuthan, R.
Tematy:
probiotics
detoxifiction therapy
radionuclides
heavy metals
mycotoxins
Pokaż więcej
Wydawca:
Instytut Chemii i Techniki Jądrowej
Powiązania:
https://bibliotekanauki.pl/articles/147297.pdf  Link otwiera się w nowym oknie
Opis:
Many applications of probiotics have been described to date. In this paper, it is hypothesized that probiotic microorganisms can also be used to decrease the xenobiotics intake in humans. The use of probiotic bacteriae (e.g. strains of Lactobacillus sp. and Bifidobacterium sp.) and yeasts (Saccharomyces sp.) gives the opportunity for detoxification of various elements and compounds, considered as contaminants, directly in the lumen of human intestine. Some of these microorganisms are known to accumulate cesium, strontium and heavy metals to a great extent and also bind mycotoxins. Certainly, during the up-coming years, their native or genetically modified strains will be a part of treatment protocols in detoxification therapy. The utilization of probiotics, in the both therapy and nutrition of people living in the countries suffering from high food contamination, could result in the reduction of annual xenobiotic dose to be incorporated in their organisms.
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 62

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