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Wyświetlanie 1-10 z 16
Tytuł:
Novel oligosaccharides isolated from Fusarium oxysporum L. rapidly induce PAL activity in Rubus cells.
Autorzy:
Nita-Lazar, Mihai
Heyraud, Alain
Gey, Claude
Braccini, Isabelle
Lienart, Yvette
Tematy:
oligosaccharide elicitors
Rubus fructicosus
phenylalanine ammonia lyase
Fusarium oxysporum
Pokaż więcej
Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1041538.pdf  Link otwiera się w nowym oknie
Opis:
Activation of the phenolic pathway is known to be part of a defense response against cell wall-derived elicitors from pathogens. Many examples of a defense response by increasing the synthesis of phenolic compound against the elicitor were demonstrated in the past, but the elicitor structure has so far been poorly characterized. Our results indicate that a disaccharide fraction containing the following structure: α-D-mannopyranosyl (1→2)α/β-D-glucopyranosyl and α-D-mannopyranosyl (1→x) inositol, isolated from Fusarium oxysporum L., promotes rapid and transient phenylalanine ammonia lyase activity in Rubus fructicosus cells at nanomolar concentration. The disaccharides were isolated by size-exclusion chromatography directly from extracts obtained by alkaline treatment of F. oxysporum mycelium. Their structure was determined by 500-MHz-1H-NMR spectroscopy combined with methylation analysis and fast atom bombardment mass spectrometry.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cyclitols, galactosyl cyclitols and raffinose family oligosaccharides in Mexican wild lupin seeds
Autorzy:
Piotrowicz-Cieslak, A I
Lopez, P.M.G.
Gulewicz, K.
Tematy:
wild lupin
oligosaccharide
lupin
carbohydrate
cyclitol
galactosyl cyclitol
Mexico
seed
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Wydawca:
Polskie Towarzystwo Botaniczne
Powiązania:
https://bibliotekanauki.pl/articles/57746.pdf  Link otwiera się w nowym oknie
Opis:
Ten to 16 ethanol-soluble carbohydrate components were identified in the seeds of six Mexican wild lupins. The analysed carbohydrates included: monosaccharides, disaccharides, cyclitols, galactosyl cyclitols and raffinose family oligosaccharides. Stachyose and sucrose were the main carbohydrate component in the Lupinus montanus, L. rotundiflorus, L. exaltatus, L. mexicanus and L. elegans seeds. Only trace quantities of verbascose were detected in Lupinus mexicanus seeds. The analysed seeds accumulated 38 to 78 mg/g d.m. carbohydrates. The raffinose family oligosaccharides constituted 41 to 85.2% of the identified carbohydrate component pool. The analysed Lupinus seeds contained 3 to 8 unidentified carbohydrate components.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of exogenous abscisic acid on accumulation of raffinose family oligosaccharides and galactosyl cyclitols in tiny vetch seeds [Vicia hirsuta [L.] S.F.Gray]
Autorzy:
Lahuta, L B
Gorecki, R.J.
Gojlo, E.
Horbowicz, M.
Tematy:
abscisic acid
vetch
oligosaccharide
botany
raffinose
Vicia hirsuta
galactosyl cyclitol
seed
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Wydawca:
Polskie Towarzystwo Botaniczne
Powiązania:
https://bibliotekanauki.pl/articles/57525.pdf  Link otwiera się w nowym oknie
Opis:
The role of the abscisic acid (ABA) in biosynthesis of raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C) in tiny vetch (Vicia hirsuta [L.] S.F. Gray) seeds was investigated. The ABA was applied through incubation of seed at various stage of its development. The level of RFOs and Gal-C was determined in seed maturing on plant and in seed maturing in vitro. In early stages of V. hirsuta seed development, the ABA activated the biosynthesis of galactinol, although the level of arisen galactinol quickly declined. In the later stages of V. hirsuta seed development ABA had stimulatory effect of RFOs and Gal-C biosynthesis. Influence of ABA on biosynthesis of a-galactosides in Vicia hirsuta seed seems to be dependent on abscisic acid concentration. Low concentration of ABA had stimulatory effect on a-galactosides biosynthesis, but high concentration of ABA inhibited the process.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inhibition of raffinose family oligosaccharides and galactosyl pinitols breakdown delays germination of winter vetch [Vicia villosa Roth.] seeds
Autorzy:
Lahuta, L B
Goszczynska, J.
Tematy:
legume seed
seed
Vicia villosa
vetch
winter vetch
galactosyl pinitol
germination
raffinose family oligosaccharide
Pokaż więcej
Wydawca:
Polskie Towarzystwo Botaniczne
Powiązania:
https://bibliotekanauki.pl/articles/59053.pdf  Link otwiera się w nowym oknie
Opis:
Beside RFOs, which are commonly present in legume seeds, seeds of some species contain galactosyl pinitols (GPs). These carbohydrates, like RFOs, have been hypothesized to constitute an important energy and carbon skeletal source during germination. To test this hypothesis we have applied a specific α-galactosidase inhibitor (1-deoxygalactonojirimycin, DGJ) to germinating winter vetch (Vicia villosa Roth.) seeds, containing more galactosyl pinitols than RFOs. The breakdown of RFOs but not that of GPs was completely blocked in both embryonic axes and cotyledons tissues, during the first 18 h of imbibition in DGJ. The inhibitor only decreased the rate of GPs degradation. The inhibitory effect of DGJ on GPs degradation was partially alleviated by addition of sucrose or galactose to DGJ solutions. After three days of germination in water, RFOs and GPs disappeared in axial tissues of seeds imbibed in water, galactose or sucrose. Eighteen-hour imbibition of seeds in DGJ drastically reduced germination, by ca 50%, during the first three days. The inhibitory effect of DGJ decreased during the next seven days of germination. The presence of galactose or sucrose in imbibition solution initially stimulated seed germination, but later this effect was not statistically significant. Our study provides clear evidence that galactosyl pinitols play an important role in early winter vetch seeds germination. Additionally, we suggest that galactosyl pinitols can replace RFOs as reserve material necessary for early germination.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Method for obtaining verbascose standard
Autorzy:
Amarowicz, R.
Ciska, E.
Kozlowska, H.
Tematy:
verbascose standard
oligosaccharide
lupin
faba bean
broad bean
high performance liquid chromatography
seed
column chromatography
Pokaż więcej
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Powiązania:
https://bibliotekanauki.pl/articles/1371731.pdf  Link otwiera się w nowym oknie
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Carbohydrates in Colobanthus quitensis and Deschampsia antarctica
Autorzy:
Piotrowicz-Cieslak, A I
Gielwanowska, I.
Bochenek, A.
Loro, P.
Gorecki, R.J.
Tematy:
soluble sugar
monosaccharide
oligosaccharide
carbohydrate
cyclitol
starch
vegetative tissue
Deschampsia antarctica
sugar
Colobanthus quitensis
raffinose
tissue
galactosyl cyclitol
Pokaż więcej
Wydawca:
Polskie Towarzystwo Botaniczne
Powiązania:
https://bibliotekanauki.pl/articles/59067.pdf  Link otwiera się w nowym oknie
Opis:
Eight to nineteen ethanol-soluble carbohydrate components were identified in vegetative tissues of Colobanthus quitensis and Deschampsia antarctica. The analysed carbohydrates included: monosaccharides, cyclitols, galactosyl cyclitols, raffinose family oligosaccharides, lichnose family oligosaccharides, kestose family oligosaccharides. The analysed vegetative tissues accumulated from 447 to 139 mg/g d.m. soluble carbohydrates in Colobanthus quitensis, Deschampsia antarctica respectively. The raffinose family oligosaccharides constituted 53.3% in Colobanthus quitensis of the identified soluble carbohydrate component pool. Vegetative tissues accumulated starch in Colobanthus quitensis 20.6 mg/g d.m. and 261.6 mg/g d.m. in Deschampsia antarctica. Anatomical and ultrastructural observations of vegetative part of Colobanthus quitensis and Deschmpsia antarctica revealed the presence of various ergastic materials in intercellular spaces, cell walls and protoplasts. Various parts of these plants contain insoluble, PAS positive polysaccharides in intercellular spaces and in cell walls. Chloroplasts of analysed tissues contained starch. Less starch was visible in young, growing parts of shoots of Colobanthus quitensis and Deschmpsia antarctica, more starch appears in mature, differentiated parts.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biosynthesis of raffinose family oligosaccharides and galactosyl pinitols in developing and maturing seeds of winter vetch [Vicia villosa Roth.]
Autorzy:
Lahuta, L B
Tematy:
biosynthesis
raffinose family
oligosaccharide
galactosyl pinitol
cyclitol
galactosyltransferase
developing seed
maturing seed
seed
winter vetch
vetch
Vicia villosa
Pokaż więcej
Wydawca:
Polskie Towarzystwo Botaniczne
Powiązania:
https://bibliotekanauki.pl/articles/56752.pdf  Link otwiera się w nowym oknie
Opis:
Changes in the accumulation of two types of α-D-galactosides: raffinose family oligosaccharides and galactosyl pinitols were compared with changes in the activities of galactosyltransferases during winter vetch (Vicia villosa Roth.) seed development and maturation. Occurrence of galactinol and raffinose in young seeds and changes in activities of galactinol synthase and raffinose synthase during seed development indicated that formation of raffinose oligosaccharides (RFOs) preceded synthesis of galactopinitols. Although transfer of galactose residues into raffinose oligosaccharides increased as seeds were maturing, at late stages of seed maturation the accumulation of galactopinitols was preferred to that of RFOs. In the present study, activities of enzymes transferring galactose moieties from galactinol to D-pinitol forming galactopinitol A, and further transfer of galactose moieties from galactinol to mono- and di-galactopinitol A were detected throughout seed development and maturation. This is a new observation, indicating biological potential of winter vetch seeds to synthesize mono-, di- and tri-galactosides of D-pinitol in a pathway similar to RFOs. The pattern of changes in activities of stachyose synthase and enzymes synthesizing galactopinitols (named galactopinitol A synthase and ciceritol synthase) suggests that formation of stachyose, mono- and di-galactopinitol A (ciceritol) is catalyzed by one enzyme. High correlation between activities of verbascose synthase and enzyme catalyzing synthesis of tri-galactopinitol A from galactinol and ciceritol (named tri-galactopinitol A synthase) also suggests that biosynthesis of both types of tri-galactosides was catalyzed by one enzyme, but distinct from stachyose synthase. Changes in concentrations of galactosyl acceptors (sucrose and D-pinitol) can be a factor which regulates splitting of galactose moieties between both types of galactosides in winter vetch seeds.
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 16

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