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Wyświetlanie 1-10 z 107
Tytuł:
Evaluation of platelet indexes as potential biomarkers of suspected pulmonary embolism
Autorzy:
Wójcik, Mariusz
Daszyk-Wójcik, Joanna
Skoczyński, Kamil
Tematy:
mean platelet volume
platelet count
platelet distribution width
pulmonary embolism
Pokaż więcej
Wydawca:
Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Powiązania:
https://bibliotekanauki.pl/articles/454999.pdf  Link otwiera się w nowym oknie
Opis:
Introduction. Pulmonary embolism is one of the most frequent cardiovascular diseases, potentially leading to death. There is no validated biomarker with both high specificity and sensitivity. Aim. The aim of the study was to define the diagnostic importance of platelet count (PLT), mean platelet volume (MPV) and platelet distribution width (PDW) on acute pulmonary embolism. Material and methods. We retrospectively reviewed the medical records of 145 patients with clinically suspected acute pulmonary embolism admitted to the Emergency Department. Demographic data and laboratory tests were collected on admission. All patients underwent computed tomography (CT) angiography. Results. The total data of 145 patients were analyzed, including 65 patients (67±17 years; 30 men/35 women) with acute pulmonary embolism confirmed with CT and 80 patients (67±19 years; 26 men/54 women) with negative CT. The MPV did not differ between the patients with acute PE and the control group (8.0 fL [IQR: 7.6-8.4] vs. 7.9 fL [IQR: 7.4-8.7], p=0.45). There were no significant differences in PLT (220x10³/mm³ [IQR: 172-274] vs. 243x10³/mm³ [IQR: 186-286], p=0.12) and PDW (59.0 ± 6.9% vs. 57.2 ± 7.3%, p=0.12). Conclusions. Our results suggest that platelet indexes (at a single time point) are not a reliable diagnostic biomarkers of acute pulmonary embolism.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inhibition of collagen-induced platelet reactivity by DGEA peptide.
Autorzy:
Luzak, Boguslawa
Golanski, Jacek
Rozalski, Marcin
Boncler, Magdalena
Watala, Cezary
Tematy:
collagen
platelet receptor antagonists
collagen receptors
GPIaIIa
DGEA
platelet reactivity
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Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1043398.pdf  Link otwiera się w nowym oknie
Opis:
Direct interactions between collagen, the most thrombogenic component of the extracellular matrix, and platelet surface membrane receptors mediate platelet adhesion and induce platelet activation and aggregation. In this process two glycoproteins are crucial: integrin α2β1, an adhesive receptor, and GPVI, which is especially responsible for signal transduction. Specific antagonists of the collagen receptors are useful tools for investigating the complexity of platelet-collagen interactions. In this work we assessed the usefulness of DGEA peptide (Asp-Gly-Glu-Ala), the shortest collagen type I-derived motif recognised by the collagen-binding integrin α2β1, as a potential antagonist of collagen receptors. We examined platelet function using several methods including platelet adhesion under static conditions, platelet function analyser PFA-100TM, whole blood electric impedance aggregometry (WBEA) and flow cytometry. We found that DGEA significantly inhibited adhesion, aggregation and release reaction of collagen activated blood platelets. The inhibitory effect of DGEA on static platelet adhesion reached sub-maximal values at millimolar inhibitor concentrations, whereas the specific blocker of α2β1 - monoclonal antibodies Gi9, when used at saturating concentrations, had only a moderate inhibitory effect on platelet adhesion. Considering that 25-30% of total collagen binding to α2β1 is specific, we conclude that DGEA is a strong antagonist interfering with a variety of collagen-platelet interactions, and it can be recognised not only by the primary platelet adhesion receptor α2β1 but also by other collagen receptors.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of some ε-aminocaproic acid derivatives on platelet responses.
Autorzy:
Bruzgo, Irena
Tomasiak, Marian
Stelmach, Halina
Midura-Nowaczek, Krystyna
Tematy:
platelet aggregation
ε-aminocaproic acid derivatives
platelet adhesion
LDH release
Pokaż więcej
Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1043320.pdf  Link otwiera się w nowym oknie
Opis:
ε-Aminocaproic acid (EACA) is a synthetic low molecular drug with antifibrinolytic activity. However, treatment with this drug can be incidentally associated with an increased thrombotic tendency. The aim of the present work was to test synthetic EACA derivatives for their antiplatelet activities. We investigated the effect of three EACA derivatives with antifibrinolytic activity: I. ε-aminocaproyl-L-leucine hydrochloride (HClH-EACA-L-Leu-OH), II. ε-aminocaproyl-L-(S-benzyl)-cysteine hydrochloride (HClH-EACA-L-Cys(S-Bzl)-OH) and III. ε-aminocaproyl-L-norleucine (H-EACA-L-Nle-OH) on platelet responses (aggregation and adhesion) and on their integrity. It was found that: 1. as judged by LDH release test, none of the tested compounds, up to 20 mM, was toxic to platelets, 2. in comparison with EACA, all the synthetic derivatives inhibited much stronger the ADP- and collagen-induced aggregation of platelets suspended in plasma (platelet rich plasma) and aggregation of these cells in whole blood, 3. EACA and its derivatives exerted a similar inhibitory effect on the thrombin-induced adhesion of platelets to fibrinogen-coated surfaces. Since platelet activation and blood coagulation are tightly associated processes, the antiplatelet properties of EACA derivatives are expected to indicate reduced thrombotic properties of these derivatives compared to EACA.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of Na+/H+ exchanger in serotonin secretion from porcine blood platelets
Autorzy:
Tomasiak, Marian
Ciborowski, Michal
Stelmach, Halina
Tematy:
platelet swelling
platelet secretion
serotonin release
Na+/H+ exchanger
platelets
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Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1041323.pdf  Link otwiera się w nowym oknie
Opis:
This study was undertaken to evaluate whether a link exists between the activation of protein kinase C (PKC), operation of Na+/H+ exchanger (NHE), cell swelling and serotonin (5-HT) secretion in porcine platelets. Activation of platelets by thrombin or phorbol 12-myristate 13-acetate (PMA), a PKC activator, initiated a rapid rise in the activity of Na+/H+ exchanger and secretion of 5-HT. Both thrombin- and PMA-evoked activation of Na+/H+ exchanger was less pronounced in the presence of ethyl-isopropyl-amiloride (EIPA), an NHE inhibitor, and by GF 109203X, a PKC inhibitor. Monensin (simulating the action of NHE) caused a dose-dependent release of 5-HT that was not abolished by GF 109203X or EGTA. Lack of Na+ in the suspending medium reduced thrombin-, PMA-, and monensin-evoked 5-HT secretion. GF 109203X nearly completely inhibited 5-HT release induced by PMA-, partly that induced by thrombin, and had no effect on 5-HT release induced by monensin. EIPA partly inhibited 5-HT release induced by thrombin and nearly totally that evoked by PMA. Electronic cell sizing measurements showed an increase in mean platelet volume upon treatment of cells with monensin, PMA or thrombin. The PMA- and thrombin-evoked rise in mean platelet volume was strongly reduced in the presence of EIPA. As judged by optical swelling assay monensin and PMA produced a rapid rise in platelet volume. The swelling elicited by PMA was inhibited by EIPA and its kinetics was similar to that observed in the presence of monensin. Hypoosmotically evoked platelet swelling did not affect platelet aggregation but significantly potentiated thrombin-evoked release of 5-HT and ATP. Taken together, these results show that in porcine platelets PKC may promote 5-HT secretion through the activation of NHE. It is hypothesized that enhanced Na+/H+ antiport may result in a rise in cell membrane tension (due to cell swelling) which in turn facilitates fusion of secretory granules with the plasma membrane leading to 5-HT secretion.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The endothelial barrier and cancer metastasis : does the protective facet of platelet function matter?
Autorzy:
Chłopicki, Stefan
Stojak, Marta
Przyborowski, Kamil
Smęda, Marta
Opis:
Overwhelming evidence suggests that platelets have a detrimental role in promoting cancer spread via platelet-cancer cell interactions linked to thrombotic mechanisms. On the other hand, a beneficial role of platelets in the preservation of the endothelial barrier in inflammatory conditions has been recently described, a phenomenon that could also operate in cancer-related inflammation. It is tempting to speculate that some antiplatelet strategies to combat cancer metastasis may impair the endogenous platelet-dependent mechanisms preserving endothelial barrier function. If the protective function of platelets is impaired, it may lead to increased endothelial permeability and more efficient cancer cell intravasation in the primary tumor and cancer cell extravasation at metastatic sites. In this commentary, we discuss current evidence that could support this hypothesis.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Tytuł:
PDGF-BB homodimer serum level – a good indicator of the severity of alcoholic liver cirrhosis
Autorzy:
Kurys-Denis, E.
Prystupa, A.
Luchowska-Kocot, D.
Krupski, W.
Bis-Wencel, H.
Panasiuk, L.
Tematy:
alcohol
liver cirrhosis
Child-Pugh score
platelet derived growth factor AA
platelet growth factor AB
platelet derived
growth factor BB
Pokaż więcej
Wydawca:
Instytut Medycyny Wsi
Powiązania:
https://bibliotekanauki.pl/articles/2085441.pdf  Link otwiera się w nowym oknie
Opis:
Introduction. Liver cirrhosis is a chronic disease in which progressive fibrosis is noted. This process leads to changed architectonics of the liver parenchyma and the appearance of regenerative nodules, all of which are caused by pathological activation of the hepatic stellate cells. This process is enhanced on a molecular level by many cytokines, with platelet-derived growth factors (PDGFs) playing the key role. Objective. The aim of the study was to assess serum concentrations of PDGFs active biodymers (PDGF-AA, PDGF-BB and PDGF-AB) in patients with alcoholic liver cirrhosis, and to correlate them with the stage of disease. Materials and method. 64 patients with alcoholic cirrhosis and a control group of 16 healthy individuals were analysed. Liver cirrhosis was determined based on clinical image, history of the patients’ alcohol consumption, laboratory findings and abdominal ultrasonography. The serum PDGF-AA, PDGF-BB and PDGF-AB concentrations were determined using ELISA kits. Results. Serum concentration of PDGF-AA and PDGF-BB homodimers increases in patients with alcoholic liver cirrhosis (p=0.034 and p<0.0001, respectively), unlike the serum concentration of PDGF-AB heterodimer (p>0.05). When the stage of the disease increases, the concentrations of PDGF-AA and PGFD-BB in blood also oncrease. Furthermore, the serum level of both PDGF-AA and PDGF-BB correlates significantly with the severity of alcoholic liver cirrhosis (measured by Pugh-Child’s scale), the correlation being stronger in the case of PDGF-BB levels than PDGF-AA (R=0.28; p=0.027 and R=0.26; p=0.038, respectively). Conclusions. The plasma levels of PDGF-AA and -BB may be indicators of alcohol-induced liver fibrosis process, and might be considered as future possible treatment targets, with PDGF-BB levels being an even better indicator than PDGF-AA levels.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inhibitory effect of resveratrol on free radical generation in blood platelets
Autorzy:
Olas, Beata
Żbikowska, Halina
Wachowicz, Barbara
Krajewski, Tadeusz
Buczyński, Andrzej
Magnuszewska, Arleta
Tematy:
resveratrol
blood platelet
free radicals
Pokaż więcej
Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1044455.pdf  Link otwiera się w nowym oknie
Opis:
Resveratrol (3,4',5-trihydroxystilbene), a compound found in many plants, has been shown to prevent coronary heart diseases and to exert a variety of antiinflammatory and anticancerogenic effects. It is effective in lowering the level of serum lipids and in inhibiting platelet aggregation. We evaluated the effect of trans-resveratrol on the production of free radicals in pig blood platelets and showed that resveratrol inhibited the production of different reactive oxygen species (O^·_2H^2O^2, singlet oxygen and organic radicals) measured by the luminol-dependent chemiluminescence in resting platelets (P < 0.05). Resveratrol inhibited also the generation of radicals in platelets activated by thrombin (P < 0.05). Treatment of platelets with resveratrol at concentrations of 6.25 and 12.5 μg/ml caused a statistically insignificant increase in the production of O^·-_2 in these cells, as measured by reduction of cytochrome c; however, at higher doses (25, 50 and 100 μg/ml) resveratrol distinctly reduced the generation of O^·-_2 in platelets (P < 0.05). We suggest that free radicals play an important role in the reduced reactivity of blood platelets induced by resveratrol.
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 107

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