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Wyświetlanie 1-10 z 85
Tytuł:
Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase
Autorzy:
Zalewska, Marta
Ożyhar, Andrzej
Kochman, Marian
Tematy:
protein-protein interaction
JHBP
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Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1039963.pdf  Link otwiera się w nowym oknie
Opis:
Juvenile hormone (JH) is essential for multiple physiological processes: it controls larval development, metamorphosis and adult reproduction. In insect hemolymph more than 99 % of JH is bound to juvenile hormone binding protein (JHBP), which protects JH from degradation by nonspecific hydrolases and serves as a carrier to supply the hormone to the target tissues. In Galleria mellonella hemolymph, JHBP is found in a complex with lipid-binding high molecular weight proteins (HMWP) and this interaction is enhanced in the presence of JH. In this report, we present studies on the interaction of JHBP with low molecular weight proteins (LMWP) in the hemolymph. Using ligand blotting we found that JHBP interacts with a protein of about 44 kDa. To identify the protein that preferentially binds JHBP, a LMWP fraction was applied to a Sepharose-bound JHBP and, after washing, the column was eluted with free JHBP acting as a specific competitor or with carbonic anhydrase as a negative control. The eluted proteins were separated by SDS/PAGE and analyzed by mass spectrometry. Isocitrate dehydrogenase was identified as a component of the supramolecular complex of JHBP with hemolymph proteins.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PDZ domains - common players in the cell signaling.
Autorzy:
Jeleń, Filip
Oleksy, Arkadiusz
Śmietana, Katarzyna
Otlewski, Jacek
Tematy:
PDZ domain
module
protein-protein interaction
signaling
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Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1043370.pdf  Link otwiera się w nowym oknie
Opis:
PDZ domains are ubiquitous protein interaction modules that play a key role in cellular signaling. Their binding specificity involves recognition of the carboxyl-terminus of various proteins, often belonging to receptor and ion channel families. PDZ domains also mediate more complicated molecular networks through PDZ-PDZ interactions, recognition of internal protein sequences or phosphatidylinositol moieties. The domains often form a tandem of multiple copies, but equally often such tandems or single PDZ domain occur in combination with other signaling domains (for example SH3, DH/PH, GUK, LIM, CaMK). Common occurrence of PDZ domains in Metazoans strongly suggests that their evolutionary appearance results from the complication of signaling mechanisms in multicellular organisms. Here, we focus on their structure, specificity and role in signaling pathways.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of sparse linear discriminant analysis for prediction of protein-protein interactions
Autorzy:
Stąpor, K.
Fabian, P.
Tematy:
sparse discriminant analysis
feature selection
protein-protein interaction
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Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Powiązania:
https://bibliotekanauki.pl/articles/95137.pdf  Link otwiera się w nowym oknie
Opis:
To understand the complex cellular mechanisms involved in a biological system, it is necessary to study protein-protein interactions (PPIs) at the molecular level, in which prediction of PPIs plays a significant role. In this paper we propose a new classification approach based on the sparse discriminant analysis [10] to predict obligate (permanent) and non-obligate (transient) protein-protein interactions. The sparse discriminant analysis [10] circumvents the limitations of the classical discriminant analysis [4, 9] in the high dimensional low sample size settings by incorporating inherently the feature selection into the optimization procedure. To characterize properties of protein interaction, we proposed to use the binding free energies. The performance of our proposed classifier is 75% ± 5%.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cryptochrome interaction networks across different tissues in Drosophila melanogaster
Autorzy:
Minervini, Giovanni
Biscontin, Alberto
Cendron, Filippo
Damulewicz, Milena
Colaianni, Davide
Mazzotta, Gabriella M.
Gregoris, Francesco
Opis:
Background: Drosophila CRYPTOCHROME (dCRY) is a blue light-sensitive protein involved in various biological processes, including photoreception, circadian rhythm regulation, synaptic plasticity, and magnetoreception. Its circadian function is strictly connected with light: upon light exposure, dCRY undergoes a conformational change, becoming active and binding to various proteins. However, it can also form complexes in the absence of light, with its function varying depending on the cell type in which it is expressed. Results: Here, we use an experimental approach based on co-immunoprecipitation followed by mass spectrometry analysis, obtaining the in vivo interactome of dCRY in two distinct cell populations - retina photoreceptors and glial cells - at two specific time points: just before lights-on (ZT0) and one hour after lights-on (ZT1). To gain deeper insights into the functional dynamics of dCRY, we constructed reliable protein-protein interaction networks in both cell types and across the two experimental conditions, revealing a complex landscape of interactions. Additionally, we explored the biological pathways associated with the identified dCRY interactors, highlighting several tissue- and time-specific enrichments. We focused on RNA-related pathways, indicating that dCRY and its interactors are involved in regulating RNA metabolism in photoreceptors at ZT0 and in glial cells at ZT1. Finally, as a case study, we further investigated the functions of the RNA-binding protein SQUID, found to interact with dCRY in both tissues. Notably, the impaired circadian locomotor behavior exhibited by Squid mutant flies accounts for the involvement of this hnRNP in the generation of the circadian rhythmicity. Conclusions: In conclusion, our work provides the first tissue- and time-specific dCRY interactome, offering valuable insights into previously unrecognized biological processes in which it may be involved. Specifically, its potential role in the regulation of RNA metabolism contributes crucial evidence concerning the relationship between the circadian clock and RNA metabolism, thereby laying the groundwork for future research in this area.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Autorzy:
Górka, Adam
Górecki, Andrzej
Figiel, Małgorzata
Opis:
YY1 is widely recognized as an intrinsically disordered transcription factor that plays a role in development of many cancers. In most cases, its overexpression is correlated with tumor progression and unfavorable patient outcomes. Our latest research focusing on the role of zinc ions in modulating YY1’s interaction with DNA demonstrated that zinc enhances the protein’s multimeric state and affinity to its operator. In light of these findings, changes in protein concentration appear to be just one element relevant to modulating YY1-dependent processes. Thus, alterations in zinc ion concentration can directly and specifically impact the regulation of gene expression by YY1, in line with reports indicating a correlation between zinc ion levels and advancement of certain tumors. This review concentrates on other potential consequences of YY1 interaction with zinc ions that may act by altering charge distribution, conformational state distribution, or oligomerization to influence its interactions with molecular partners that can disrupt gene expression patterns.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Wyświetlanie 1-10 z 85

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