Temat: recombination - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: Determination of initial recombination of ions created in quasi-pulsed radiation fields
Autorzy:: Zielczyński, M.
Golnik, N.
Gryziński, M. A.
Tematy:: initial recombination
volume recombination
ionization chambers
pulse radiation; Pokaż więcej
Wydawca:: Instytut Chemii i Techniki Jądrowej
Powiązania:: https://bibliotekanauki.pl/articles/146942.pdf Link otwiera się w nowym oknie
Opis:: A new method is proposed for the determination of ion collection efficiency due to initial recombination process only, in a high pressure ionization chamber, operating in a quasi-pulsed radiation field. It is shown that the influence of undesirable volume recombination can be eliminated using a semi-empirical formula. The unknown function of the chamber polarizing voltage, included to the formula, can be found experimentally in the investigated radiation field, using the approach proposed in the paper. The function is the same for all radiation fields with similar time structure.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Two types of non-homologous RNA recombination in brome mosaic virus
Autorzy:: Alejska, Magdalena
Malinowska, Nelli
Urbanowicz, Anna
Figlerowicz, Marek
Tematy:: non-homologous RNA recombination
site-specific RNA recombination
heteroduplex-mediated RNA recombination
brome mosaic virus; Pokaż więcej
Wydawca:: Polskie Towarzystwo Biochemiczne
Powiązania:: https://bibliotekanauki.pl/articles/1041326.pdf Link otwiera się w nowym oknie
Opis:: Non-homologous RNA recombination is a process enabling the exchange of genetic material between various (related or unrelated) RNA-based viruses. Despite extensive investigations its molecular mechanism remains unclear. Studies on genetic recombination in brome mosaic virus (BMV) have shown that local hybridization between genomic RNAs induces frequent non-homologous crossovers. A detailed analysis of recombinant structures suggested that local complementary regions might be involved in two types of non-homologous recombination in BMV: site-specific and heteroduplex-mediated. To verify the above hypothesis and better recognize the mechanism of the phenomenon studied we have tested how the putative types of recombination are affected by a specific mutation in the BMV polymerase gene or by changes in RNA structure. The experiments undertaken revealed substantial differences between site-specific and heteroduplex-mediated recombination, indicating that they occur according to different mechanisms. The former can be classified as homology-assisted, and the latter as homology-independent. In addition to local RNA/RNA hybridization, short regions of homology are required for site-specific crossovers to occur. They are most efficiently mediated if one homologous sequence is located at the beginning of and the second just before a double-stranded region. At present it is difficult to state what is the mechanism of heteroduplex-mediated recombination. Earlier it was postulated that strong RNA/RNA interaction enforces template switching by the viral replicase. There are, however, several observations questioning this model and indicating that some other factors, which are still unknown, may influence heteroduplex-mediated crossovers.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Evidence for RNA recombination between distinct isolates of Pepino mosaic virus
Autorzy:: Hasiów-Jaroszewska, Beata
Kuzniar, Arnold
Peters, Sander
Leunissen, Jack
Pospieszny, Henryk
Tematy:: genetic diversity
recombination
PepMV; Pokaż więcej
Wydawca:: Polskie Towarzystwo Biochemiczne
Powiązania:: https://bibliotekanauki.pl/articles/1040390.pdf Link otwiera się w nowym oknie
Opis:: Genetic recombination plays an important role in the evolution of virus genomes. In this study we analyzed publicly available genomic sequences of Pepino mosaic virus (PepMV) for recombination events using several bioinformatics tools. The genome-wide analyses not only confirm the presence of previously found recombination events in PepMV but also provide the first evidence for double recombinant origin of the US2 isolate.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Initial recombination of ions in ionization chambers filled with hydrocarbon gases
Autorzy:: Gryziński, M.
Golnik, N.
Zielczyński, M.
Tematy:: initial recombination
ionization chambers; Pokaż więcej
Wydawca:: Instytut Chemii i Techniki Jądrowej
Powiązania:: https://bibliotekanauki.pl/articles/148268.pdf Link otwiera się w nowym oknie
Opis:: Data on the initial recombination of ions are needed for designing recombination chambers, used in mixed radiation dosimetry in order to obtain information about radiation quality. Nevertheless, experimental data for pressurized gases are scarce, whereas reliable theoretical models are absent. The paper provides experimental data on the initial recombination in hydrocarbon gases (methane, ethane and propane) depending on gas pressure up to 1.8 MPa. The data were derived from the measurements of saturation curves of a tissue equivalent recombination chamber of an REM-2 type, in reference radiation fields of 137Cs and 239Pu-Be sources. Ion recombination due to volume recombination and back diffusion of ions was subtracted from the measured values of ion collection efficiency. The values of gas parameters needed for the subtraction procedure were determined by measurements of saturation curves of the chamber at several dose rates for each gas pressure. The important practical rule resulting from the experiments is that in the first approximation the initial recombination does not depend on the kind of the hydrocarbon gas but only on its density in the chamber.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Studies of recombination chambers filled with nitrogen for BNCT dosimetry
Autorzy:: Tulik, P.
Golnik, N.
Tematy:: recombination chamber
BNCT
dose components; Pokaż więcej
Wydawca:: Instytut Chemii i Techniki Jądrowej
Powiązania:: https://bibliotekanauki.pl/articles/148169.pdf Link otwiera się w nowym oknie
Opis:: Dosimetric characterization of therapy beams for boron neutron capture therapy (BNCT) involves determination of dose components and among them the “nitrogen” dose due to protons generated by neutron capture on 14N. In this work, investigations were carried out using a graphite recombination chamber in order to determine the 14N capture, gamma, and fast neutron dose components. The separation of the dose components is based on differences in the shape of the saturation curve, depending on the LET spectrum of the investigated radiation. The measurements were performed in reference radiation fields at the Institute of Atomic Energy at Świerk and at a reactor beam of the INP Řež (the Czech Republic). The gamma component was determined with an accuracy of about 5%, while the variations in its value could be monitored with an accuracy of about 0.5%. Relative changes in the beam components (thermal/fast neutrons) could be detected on line with an accuracy of about 5%. It was shown that the chamber with tissue-equivalent cups could be used for the determination of the 14N capture dose at different depths in tissue.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Ambient dose equivalent measurements in secondary radiation fi elds at proton therapy facility CCB IFJ PAN in Krakow using recombination chambers
Autorzy:: Jakubowska, E. A.
Gryziński, M. A.
Golnik, N.
Tulik, P.
Stolarczyk, L.
Horwacik, T.
Zbroja, K.
Góra, Ł.
Tematy:: recombination chambers
workplace monitoring
proton therapy; Pokaż więcej
Wydawca:: Instytut Chemii i Techniki Jądrowej
Powiązania:: https://bibliotekanauki.pl/articles/147396.pdf Link otwiera się w nowym oknie
Opis:: This work presents recombination methods used for secondary radiation measurements at the Facility for Proton Radiotherapy of Eye Cancer at the Institute for Nuclear Physics, IFJ, in Krakow (Poland). The measurements of H*(10) were performed, with REM-2 tissue equivalent chamber in two halls of cyclotrons AIC-144 and Proteus C-235 and in the corridors close to treatment rooms. The measurements were completed by determination of gamma radiation component, using a hydrogen-free recombination chamber. The results were compared with the measurements using rem meter types FHT 762 (WENDI-II) and NM2 FHT 192 gamma probe and with stationary dosimetric system.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Improved dosimetry for BNCT by activation foils, modified thermoluminescent detectors and recombination chambers
Autorzy:: Bilski, P.
Golnik, N.
Olko, P.
Pytel, K.
Tracz, G.
Tulik, P.
Zielczyński, M.
Tematy:: BNCT
recombination chambers
TLD
activation foils; Pokaż więcej
Wydawca:: Instytut Chemii i Techniki Jądrowej
Powiązania:: https://bibliotekanauki.pl/articles/971519.pdf Link otwiera się w nowym oknie
Opis:: Boron neutron capture therapy (BNCT) is an experimental therapy of selected tumours, based on a nuclear reaction initiated by the capture of thermal neutron by the 10B nucleus. After 10B had been delivered selectively to tumour cells, it can be activated by neutrons to deliver locally lethal high-LET radiation. BNCT beams are complex mixed radiation fields, because of broad neutron energy range, presence of gamma contamination and necessity of precise determination of several dose components. The paper presents
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Sp100 interacts with phage ΦC31 integrase to inhibit its recombination activity
Autorzy:: Lin, Yun
Li, Zhi-Hui
Wang, Jing-Jing
Xu, Gua-Lan
Shen, Qi
Tian, Lin
Xue, Jin-Lun
Chen, Jin-Zhong
Tematy:: Sp100
ΦC31 integrase
recombination; Pokaż więcej
Wydawca:: Polskie Towarzystwo Biochemiczne
Powiązania:: https://bibliotekanauki.pl/articles/1039951.pdf Link otwiera się w nowym oknie
Opis:: Phage ΦC31 integrase is a potential vector for the insertion of therapeutic genes into specific sites in the human genome. To understand the mechanism involved in ΦC31 integrase-mediated recombination, it is important to understand the interaction between the integrase and cellular proteins. Using a yeast two-hybrid system with pLexA-ΦC31 integrase as bait, we screened a pB42AD human fetal brain cDNA library for potential interacting cellular proteins. From the 106 independent clones that were screened, 11 potential interacting clones were isolated, of which one encoded C-terminal fragment of Sp100. The interaction between Sp100 and ΦC31 integrase was further confirmed by yeast mating and co-immunoprecipitation assays. The hybridization between a ΦC31 integrase peptide array and an HEK293 cell extract revealed that residues 81RILN84 in the N-terminus of ΦC31 integrase are responsible for the interaction with Sp100. Knocking down endogenous Sp100 with Sp100-specific siRNA increased ΦC31 integrase-mediated recombination but did not impact reporter gene expression. Therefore, endogenous Sp100 may interact with ΦC31 integrase and inhibit the efficiency of ΦC31 integrase-mediated recombination.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Detection of recombinant mitochondrial genomes: Implications for the mechanism of mtDNA inheritance in mussel Mytilus
Autorzy:: Filipowicz, M.
Burzyński, A.
Wenne, R.
Tematy:: DNA mitochondrialne
rekombinacja
Mytilus
mitochondrial DNA
recombination; Pokaż więcej
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Powiązania:: https://bibliotekanauki.pl/articles/363232.pdf Link otwiera się w nowym oknie
Opis:: Recombination plays a fundamental role in the creation of biodiversity. It is the mechanism inducing formation of rearrangements within the genomes which, beside mutations, are the major source of genetic variation. In the process of recombination a single or double DNA strand is broken and rejoined with unassociated DNA fragments. There are several types of recombination: homologous recombination, sitespecific recombination and transposition. Within mitochondrial genomes, inter- and intra-molecular recombination can occur. Except for intramolecular recombination of mtDNA, the other types of recombination always result in the creation of mosaic genomes. However, in the natural populations mtDNA recombination is detected extremely rarely. It is caused by the clonal inheritance of mitochondrial genomes and consequential lack of sufficient divergence between parental mitochondrial molecules. Mussels of the genus Mytilus possess two types of mitochondrial genomes inherited from males and females, respectively, and their mode of mtDNA inheritance is called doubly uniparental inheritance (DUI). The presence of two highly diverged parental molecules gives the opportunity for detection of recombinant variants. This feature of Mytilus mtDNA can be broadly exploited in the search for and characteristics of recombinant sequences. Apart from the high level of sequence divergence, fusion of mitochondria and appropriate enzymatic toolkit are principal requirements for the occurrence of recombination. The majority of phylogenetic and demographic analysis based on mtDNA assumes the lack of recombination. If this assumption turned out to be erroneous, previous analyses would be weakened. Recombination is associated with DUI abnormalities, e.g. masculinization of mitochondrial genomes. It may even lead to the breakdown of DUI system resulting in the new, unidentified mode of mtDNA inheritance in mussel Mytilus that might be regulated by stochastic events.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Calculated neutron energy dependence of the dose-response of large recombination chamber
Autorzy:: Tymińska, Katarzyna
Gryziński, Michał A.
Maciak, Maciej
Tematy:: ambient absorbed dose
Monte Carlo
recombination chamber; Pokaż więcej
Wydawca:: Instytut Chemii i Techniki Jądrowej
Powiązania:: https://bibliotekanauki.pl/articles/146700.pdf Link otwiera się w nowym oknie
Opis:: A model of REM-2-type chamber was modeled with MCNPX code to study the dose-response to monoenergetic neutrons in wide energy range from thermal to 20 MeV for various compositions of gas in the chamber. The energy dependence of the total dose absorbed in the fi lling gas was compared with the energy dependence of ambient absorbed dose D*(10) and with experimental data. The results of the studies will be useful for designing new, improved generation of recombination chambers.
Dostawca treści:: Biblioteka Nauki

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