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Artykuł
Tytuł:
Prospective observational study of adenoidal biofilms in a paediatric population and its clinical implications
Autorzy:
Subtil, Joao
Bajanca-Lavado, Maria Paula
Rodrigues, Joao
Duarte, Aida
Reis, Lucia
Nogueira, Isabel
Jordao, Luisa
Tematy:
Adenoids
biofilms
scanning electron microscopy
Haemophilus
child.
Pokaż więcej
Wydawca:
Index Copernicus International
Powiązania:
https://bibliotekanauki.pl/articles/1397787.pdf  Link otwiera się w nowym oknie
Opis:
INTRODUCTION: Adenoids are nasopharyngeal lymphoid tissue with a relevant role in host defence against infection of upper respiratory tract. Nevertheless, adenoids are also a reservoir of microorganisms that can cause infections of upper respiratory tract and otitis particularly in children. OBJECTIVE: Evaluate and compare the association between biofilm assembly on adenoids and the incidence of recurrent infections in a paediatric population submitted to adenoidectomy by either infectious or non-infectious indication. METHODS: Scanning electron microscopy was used to assess biofilms on adenoid surface; biofilm assembly in vitro was monitored by crystal violet assay; antibiotic susceptibility was assessed following EUCAST guidelines; Hinfluenzae capsular typing was performed by PCR. RESULTS: Biofilms were present in 27.4% of adenoid samples and no statistical difference was found between infectious and non-infectious groups. In vitro, the most clinically relevant bacteria, H.influenzae, S.aureus, S.pyogenes, S.pneumoniae and M.catarrhalis, were mostly moderate biofilm assemblers (71.7%). 55.3% of these bacteria were intermediate/resistant to at least one of the tested antibiotics. No association was found between the ability to assemble biofilms in vitro and the presence of biofilms on adenoids nor antibiotic resistance. All H.influenzae were characterized as non-typeable. CONCLUSION: The presence of biofilms on adenoid surface was independent from clinical sample background. Bacterial ability to assemble biofilms in vitro cannot be used to predict biofilm assembly in vivo. The lack of correlation between biofilm formation and infectious respiratory diseases found contributes to question the relevance of biofilms on the pathogenesis of infectious diseases.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Erigeron acris L. subsp. angulosus (Gaudin) Vacc. (Asteraceae), a new taxon in the flora of Poland
Autorzy:
Pliszko, Artur
Opis:
The paper reports for the first time the occurrence of Erigeron acris subsp. angulosus in Poland. This rare European temperate plant was found in August 2014 in a former sand and gravel quarry, close to the Sobolewo reservoir in the town of Suwałki, north-eastern Poland. Species composition of the habitat is characterized by a phytosociological relevé based on the Braun-Blanquet method, diagnostic characters in comparison to the morphologically similar E. acris subsp. droebachiensis are presented using scanning electron microscopy imaging, and an identification key for E. acris s. l. in Poland is given.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Tytuł:
Deformation Mechanisms and Fracture of Ni-Based Metallic Glasses
Autorzy:
Lesz, S.
Griner, S.
Nowosielski, R.
Tematy:
metals
transmission electron microscopy
scanning electron microscopy
fracture
shear bands
Pokaż więcej
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/353790.pdf  Link otwiera się w nowym oknie
Opis:
The cracking of materials and fracture surface is of great practical and academic importance. Over the last few years the development of the fractography of crystalline alloys resulted in a useful tool for the prediction or failure analysis. Many attempts have been made to observe cracks using optical microscopy, X-ray topography and transmission electron microscopy (TEM). Of these techniques, the resolution of optical microscopy and X-ray topography is too poor. By contrast, the resolution of TEM is high enough for detailed information to be obtained. However, in order to apply TEM observations, a thin foil specimen must be prepared, and it is usually extremely difficult to prepare such a specimen from a pre-selected region containing a crack. In the present work, deformation mechanisms fracture surfaces of Ni-based metallic glass samples have been studied by specially designed experiments. In order to study the deformation mechanisms and fracture the Ni-based metallic glasses have been investigated in the tensile test. The structure and fracture surfaces after the decohesion process in tensile tests were observed using transmission electron microscope (TEM) and scanning electron microscope (SEM), respectively. The studies of structure were performed on thin foils. Moreover the investigated tape was subjected to a banding test. Then, the tape was straightened and the thin foil from the area of maximum strain was prepared. This thin foil sample was deformed before the TEM investigation to obtain local tears.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Milling Time on Amorphization of Mg-Zn-Ca Powders Synthesized by Mechanical Alloying Technique
Autorzy:
Lesz, S.
Kremzer, M.
Gołombek, K.
Nowosielski, R.
Tematy:
Mg-based powders
transmission electron microscopy
scanning electron microscopy
mechanical alloying
Pokaż więcej
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:
https://bibliotekanauki.pl/articles/354717.pdf  Link otwiera się w nowym oknie
Opis:
Mg60 Zn35 Ca5 amorphous powder alloys were synthesized by mechanical alloying (MA) technique. The results of the influence of high-energy ball-milling time on amorphization of the Mg60 Zn35 Ca5 elemental blend (intended for biomedical application) were presented in the study. The amorphization process was investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM). Initial elemental powders were mechanically alloyed in a Spex 8000 high-energy ball mill at different milling times (from 3 to 24 h). Observation of the powder morphology after various stages of milling leads to the conclusion that with the increase of the milling time the size of the powder particles as well as the degree of aggregation change. The partially amorphous powders were obtained in the Mg60 Zn35 Ca5 alloy after milling for 13-18h. The results indicate that this technique is a powerful process for preparing Mg60 Zn35 Ca5 alloys with amorphous and nanocrystalline structure.
Dostawca treści:
Biblioteka Nauki
Artykuł

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