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Wyświetlanie 1-10 z 139
Tytuł:
The role of benzoate secreted by Desulfotomaculum acetoxidans DSM 771 in sulfate uptake
Autorzy:
Pawłowska-Ćwięk, Lucyna
Pado, Ryszard
Tematy:
sulfonation
benzoate secretion
oxidation
Pokaż więcej
Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1041319.pdf  Link otwiera się w nowym oknie
Opis:
This work was designed to find the cause of the delay in hydrogen sulfide dissimilation in Desulfotomaculum acetoxidans DSM 771, which is dependent on the sulfate uptake. This bacterium grown without addition of any aromatic compound was shown by spectrum analysis with the methylene method to contain hydroxy-benzoate derivatives. The presence of these compounds was confirmed by HPLC in fractions obtained from cell walls after 15 days of culture. The test with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt seemed to indicate the presence of peroxidase, which probably oxidized benzoate to its hydroxy derivatives. The test with 5-sulfo-salicylic acid proved the ability of the investigated strain to utilize arylsulfates and to reduce sulfate group to hydrogen sulfide. On the basis of the above data, we propose the following sequence of reactions: 1, benzoate secretion; 2, benzoate hydroxylation; 3, sulfonation of hydroxy-benzoate derivatives.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Alterations in protein secretion caused by metabolic engineering of glycosylation pathways in fungi
Autorzy:
Kruszewska, Joanna
Perlińska-Lenart, Urszula
Górka-Nieć, Wioletta
Orłowski, Jacek
Zembek, Patrycja
Palamarczyk, Grażyna
Tematy:
glycosylation
protein secretion
Trichoderma
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Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1040697.pdf  Link otwiera się w nowym oknie
Opis:
Due to its natural properties, Trichoderma reesei is commonly used in industry-scale production of secretory proteins. Since almost all secreted proteins are O-glycosylated, modulation of the activity of enzymes of the O-glycosylation pathway are likely to affect protein production and secretion or change the glycosylation pattern of the secreted proteins, altering their stability and biological activity. Understanding how the activation of different components of the O-glycosylation pathway influences the glycosylation pattern of proteins and their production and secretion could help in elucidating the mechanism of the regulation of these processes and should facilitate creation of engineered microorganisms producing high amounts of useful proteins. In this review we focus on data concerning Trichoderma, but also present some background information allowing comparison with other fungal species.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gular gland of the Indian sheath-tailed bat
Autorzy:
Pandey, S. D.
Dominic, C. J.
Powiązania:
Acta Theriologica
Opis:
The morphology, histology and histochemistry of the gular gland of the Indian sheath-tailed bat, Taphozous longimanus Hardwicke, 1823, are described. A gular gland which is presented only in the male is a flattened, pear-shaped structure laying below the skin on the ventral side of neck.
Dostawca treści:
RCIN - Repozytorium Cyfrowe Instytutów Naukowych
Książka
Tytuł:
Analysis of individual azurocidin N-glycosylation sites in regard to its secretion by insect cells, susceptibility to proteolysis and antibacterial activity
Autorzy:
Indyk, Katarzyna
Olczak, Teresa
Ciuraszkiewicz, Justyna
Wątorek, Wiesław
Olczak, Mariusz
Tematy:
glycosylation
protein secretion
azurocidin
antimicrobial activity
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Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1041040.pdf  Link otwiera się w nowym oknie
Opis:
Azurocidin is an inactive serine protease homolog with primary sequence similarity to neutrophil elastase, cathepsin G, and proteinase 3. The aim of this study was to investigate possible consequences of differential glycosylation of azurocidin in regard to its secretion, protein stability as measured by susceptibility to proteolysis, and antibacterial activity. Site-directed mutagenesis was employed to generate mutant azurocidin variants lacking individual N-glycosylation sites. Our results show that N-linked glycans may play a role in proper azurocidin folding and subsequent secretion by insect cells. We also demonstrate that N-linked glycosylation contributes to azurocidin stability by protecting it from proteolysis. The lack of N-glycosylation at individual sites does not significantly influence the azurocidin antibacterial activity.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of the low-frequency magnetic fields of different parameters on the secretion of cortisol in men
Autorzy:
Woldańska-Okońska, Marta
Czernicki, Jan
Karasek, Michał
Tematy:
magnetic fields
cortisol secretion
stressogenic effect
Pokaż więcej
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Powiązania:
https://bibliotekanauki.pl/articles/2179794.pdf  Link otwiera się w nowym oknie
Opis:
Objectives: The aim of this paper is to test the infl uence of long-term application of the low-frequency magnetic fi elds in magnetotherapy and magnetostimulation on cortisol secretion in men. Materials and Methods: Patients were divided into three groups: 16 men underwent magnetotherapy and 20 men (divided into two groups) underwent magnetostimulation. Magnetotherapy – 2 mT induction, 40 Hz, bipolar square wave, was applied for 20 min to lumbar area. Magnetostimulation (Viofor Jaroszyk, Paluszak, Sieroń (JPS) system, M2P2 program) was applied to 10 patients for 12 min each day. The third group (10 patients) underwent magnetostimulation (Viofor JPS system, M3P3) for 12 min each day using a different machine. All groups had 15 rounds of applications at approximately 10:00 a.m. with intermissions on the weekends. Blood serum was taken four times in a 24-hour period, before applications, the day after applications and a month later. Chemiluminescence micromethod was used to indicate hormone concentrations. Data was statistically analyzed with the analysis of variance (ANOVA) method. Results: The statistically signifi cant gains in the circadian cortisol profi le at 4:00 p.m., before and after application, were observed as a decrease in concentration during magnetotherapy. In magnetostimulation, with the M2P2 program, a signifi cant increase in the cortisol concentration was observed in circadian profi le at 12:00 p.m. one month after the last application. After magnetostimulation with the M3P3 program, a signifi cant increase in concentration at 6:00 a.m. and a decrease in concentration at 12:00 p.m. were observed one month later. Statistically signifi cant difference was demonstrated in the participants after the application of magnetotherapy and magnetostimulation with M3P3 program compared to the men submitted to magnetostimulation, with M2P2 program, at 4:00 p.m. after 15 applications. Conclusions: Biological hysteresis one month after magnetostimulation suggests long-term infl uence on the hypothalamohypophysial axis. The circadian curves of cortisol secretion a day after magnetotherapy and magnetostimulation with M3P3 program compared to magnetostimulation with M2P2 progam differs nearly by 100%, which proves that they show varied infl uence on cortisol secretion in men. All changes in the hormone concentration did not exceed the physiological standards of cortisol secretion, which suggests a regulating infl uence of magnetic fi elds on cortisol concentration rather than a strong stressogenic impact of magnetostimulation.
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 139

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