Temat: seroprevalence - Prolib Integro

Skocz do pozycji: 1.

Tytuł:: Serodiagnosis of Toxoplasmosis using Lateral Flow Chromatographic Immunoassay among Animals and Humans in Sunsari District of Nepal
Autorzy:: Sah, Ramesh Prasad
Talukder, Md Hasanuzzaman
Rahman, A. K. M. Anisur
Tematy:: Animals
Nepal
human
seroprevalence
toxoplasmosis; Pokaż więcej
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Powiązania:: https://bibliotekanauki.pl/articles/1167051.pdf Link otwiera się w nowym oknie
Opis:: Toxoplasmosis is a cosmopolitan parasitic zoonosis, infecting human and other warm blooded animals worldwide. This disease has economic importance in regard to animal reproduction, and it leads to abortions and neonatal complications in humans. This study was carried out to determine the seroprevalence of Toxoplasma gondii in sheep, cattle, cats and human in Inaruwa and surrounding areas of Sunsari district, Nepal. Altogether 336 blood samples, of which 50 from sheep, 92 from cattle, 44 from cats and 150 from human were collected and tested immediately using lateral flow chromatographic immunoassay (Toxo IgG/IgM Combo Rapid test®). Associated biometric information such as age, sex, pregnancy status, occupation, association with cat was recorded and analyzed to determine the association of risk factors with the disease. Data were analyzed using R 3.2.2 (The R foundation for Statistical Computing, 2015). Seroprevalence of toxoplasmosis was detected 12.00% (95% CI: 4.53- 24.31%) in sheep, 8.70% (95% CI: 3.83- 16.42%) in cattle, 36.36% (95%CI: 22.41- 52.23%) in cats and 12.67% (95% CI: 7.80- 19.07%) in human. In case of human, 31 to 45 years age group were found more susceptible to toxoplasmosis (21.74%, OR: 6.4) in comparison to 21 to 30 years (10.0%) and up to 20 years (4.17%) age groups. Toxoplasmosis was found highly significantly associated with abortion (58.33%, OR= 15.4, P=0.0001) in human in the tested individuals. Regarding occupation, 20.83% butchers were seropositive followed by farmers (15.52%), housewives (10.0%) and diagnostic lab technicians (8.0%). Female and higher age group showed high prevalence of toxoplasmosis in all studied species. Therefore, this assay is a useful method for the serological screening of toxoplasmosis in different animals and humans.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Feline ocular toxoplasmosis: seroprevalence, diagnosis and treatment outcome of 60 clinical cases
Autorzy:: Ali, K.M.
Abu-Seida, A.M.
Abuowarda, M.
Tematy:: cats
chorioretinitis
keratitis
seroprevalence
Toxoplasma gondii
uveitis; Pokaż więcej
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Powiązania:: https://bibliotekanauki.pl/articles/2087214.pdf Link otwiera się w nowym oknie
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Occurrence of emerging ruminant viruses in goats in Poland
Autorzy:: Larska, M.
Socha, W.
Rola, J.
Tematy:: goat
seroprevalence
emerging viruses
BTV
PPRV
SBV
capripoxviruses; Pokaż więcej
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Powiązania:: https://bibliotekanauki.pl/articles/16647511.pdf Link otwiera się w nowym oknie
Opis:: Health status of Polish goat population in regard to the viral diseases remained mostly unknown. In order to determine serological status of Polish goats for selected emerging ruminant viruses, 365 serum samples collected between 2017 and 2019 in 36 districts within 10 of Polish provinces, were tested. No antibodies specific to Peste de Petite Ruminants Virus (PPRSV) and capripoxviruses (CaPV) were found in any of the tested animals. Only single individual (0.27%) was seropositive to Blutongue Virus (BTV). Antibodies directed to Schmallenberg Virus (SBV) were detected in 46 goats which represented 12.6% of the tested population. No association between seropositivity to SBV and year of sampling, province of origin, gender and age was found. In conclusion, among studied viral pathogens, currently only SBV seemed to be important for epidemiological status of Polish goats.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Serological cross-sectional survey of equine infectious anemia in Saudi Arabia
Autorzy:: Kasem, S.
Hashim, O.
Alkarar, A.
Hodhod, A.
Elias, A.
Abdallah, M.
Al-Sahaf, A.
Al-Doweriej, A.
Qasim, I.
Abdel-Moneim, A.S.
Tematy:: equine infectious anaemia
equine
ELISA
Saudi Arabia
seroprevalence; Pokaż więcej
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Powiązania:: https://bibliotekanauki.pl/articles/16539306.pdf Link otwiera się w nowym oknie
Opis:: The equine infectious anaemia virus (EIAV) is one of the most serious equine diseases worldwide. There is scarce information on the epizootiology of equine infectious anaemia (EIA) in Saudi Arabia. Given the importance of the equine industry in Saudi Arabia, this cross- -sectional study aims to provide information about the prevalence of EIAV based on serological surveillance of the equine population in the country. A total of 4728 sera samples were collected (4523 horses and 205 donkeys) between December 2017 and November 2019. All samples were tested using commercially available EIAV ELISA. All tested samples showed negative results for EIAV antibodies with a 95% confidence interval. The results provided evidence that Saudi Arabia’s equine populations (horses and donkeys) are currently free of EIAV. The results also suggest the need for continuous monitoring of EIAV and strict regulation when importing horses from other countries.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
Autorzy:: Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Zając, V.
Cencek, T.
Tematy:: Polska
PCR
seroprevalence
Toxoplasma gondii
IFAT
cats
oocysts
coproscopy; Pokaż więcej
Wydawca:: Instytut Medycyny Wsi
Powiązania:: https://bibliotekanauki.pl/articles/2081746.pdf Link otwiera się w nowym oknie
Opis:: Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Seroepidemiological study of canine Leishmania infantum and Toxoplasma gondii infections in Shanghai, China, and analysis of risk factors
Autorzy:: Jiang, Wei
Wang, Yan
Liu, Yingchun
Li, Tao
Chen, Yongjun
Wang, Shaohui
Han, Xiangan
Wang, Quan
Tematy:: seroprevalence
leishmania infantum
toxoplasma gondii
household dogs
risk factors; Pokaż więcej
Wydawca:: Instytut Medycyny Wsi
Powiązania:: https://bibliotekanauki.pl/articles/990968.pdf Link otwiera się w nowym oknie
Opis:: The aim of this study was to determine the seroprevalence of Leishmania infantum and Toxoplasma gondii among household dogs in Shanghai (the most important industrial and commercial city in China), and to assess the possible risk factors associated with the infection. During 2014–2015, a total of 408 sera were collected from healthy household dogs and tested for L. infantum and T. gondii infection using commercial ELISA kits. The endemic characteristics according to gender, age group and breed were revealed by statistical descriptions and inference. The positive rates of L. infantum infection (24/408, 5.9%) were lower than those of T. gondii infection (37/408, 9.1%), and co-infection with both parasites was detected in seven dogs (7/408, 1.7%). Seropositivity for either parasite was more likely associated with age: the seroprevalence of T. gondii infection ranged from 1.3% (dogs≤1 year) – 18.7% (dogs>6 years), whereas that of L. infantum ranged from 1.3% (dogs≤1 year) – 9.9 % (dogs>6 years). Interestingly, the rates of exposure to both L. infantum and T. gondii were higher in males than in females. Relatively higher exposure rates for L. infantum and T. gondii were also observed in crossbred dogs compared with purebred dogs. However, neither gender nor breed is likely a determining factor for infection with these two parasites (P > 0.05). Identification of the risk factors that underlie these differences may help in the prevention of L. infantum and T. gondii infection in household dogs. To the best of our knowledge, this is the first report of L. infantum and T. gondii infection in household dogs in Shanghai, which shows that these two important parasites are still prevalent in this region. Therefore, it is necessary to take integrated strategies for prevention and control of infection in animals, which could help to reduce human infection in the region.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Seroprevalence and occupational risk survey for Coxiella burnetii among exposed workers in Sicily, Southern Italy
Autorzy:: Fenga, Concettina
Gangemi, Silvia
De Luca, Annamaria
Calimeri, Sebastiano
Lo Giudice, Daniela
Pugliese, Michela
Licitra, Francesca
Alibrandi, Angela
Costa, Chiara
Tematy:: seroprevalence
Coxiella burnetii
occupational hazard
anthropozoonosis
health surveillance
prevention; Pokaż więcej
Wydawca:: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Powiązania:: https://bibliotekanauki.pl/articles/2177166.pdf Link otwiera się w nowym oknie
Opis:: Objectives The aim of this survey was to assess the seroprevalence of antibodies against Coxiella burnetii (C. burnetii) in subjects at risk of exposure in Sicily, Southern Italy. Material and Methods Prevalence of IgG antibodies to C. burnetii phase II antigens was evaluated by ELISA in a group of 140 workers at risk of exposure (38 veterinarians, 38 slaughterhouse workers, 44 livestock handlers, 20 laboratory and technical personnel) included in a medical surveillance program and in 42 control subjects. Positive samples were classified as suggestive of prior exposure to C. burnetii. Results Antibodies against C. burnetii were detected in 88 out of 140 (62.9%) exposed workers and in 6 out of 42 (14.3%) subjects of the control group. The variables evaluated did not seem to have a significant effect on seropositivity to Coxiella with the exception of symptoms in the last 6 months preceding the survey. Conclusions Our study demonstrated a high seroprevalence of C. burnetii in the group of exposed workers in comparison to non-exposed subjects of the control group. Clinical illness appears to be rare; nevertheless, physicians should consider Q fever in patients with compatible symptoms and occupational exposure to animals and their products. As aerosols represent the main route of infection in animals and humans, these workers are strongly advised to wear respiratory masks. In addition, occupational physicians should consider routine serologic evaluation and vaccination of occupationally exposed workers.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Determinants of seropositivity for SARS-CoV-2 in hospital staff in the second wave of the pandemic in Slovenia
Autorzy:: Socan, Maja
Prosenc, Katarina
Kukec, Andreja
Zaletel-Kragelj, Lijana
Remec, Tatjana
Grmek-Kosnik, Irena
Tematy:: seroprevalence
nurse
clinician
healthcare professional
COVID-19
SARS-CoV-2; Pokaż więcej
Wydawca:: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Powiązania:: https://bibliotekanauki.pl/articles/2152978.pdf Link otwiera się w nowym oknie
Opis:: Objectives The pandemic caused by the novel coronavirus (SARS-CoV-2) affected a disproportionately high percentage of healthcare workers (HCWs). The aim of the study was to assess the seroprevalence of SARS-CoV-2-specific IgG antibodies in nurses and clinicians working in 2 Slovenian regional hospitals, and to identify the factors associated with seropositivity. Material and Methods The study was designed as a crosssectional study. Clinicians and nurses were invited to participate in November–December 2020. The respondents (813, 65.8%) completed a questionnaire and consented to provide 10 ml of blood for determining the presence of SARS-CoV-2 IgG antibodies. Results The authors observed a seroprevalence rate of 20.4%. The results of the univariate analysis proved that the age of a nurse or clinician was the factor most strongly associated with seropositivity – in fact, the youngest nurses and clinicians were 8.33 times more likely to be seropositive than those in the oldest age group (p = 0.041). Being in contact with a family/household member who was SARS-CoV-2-positive was also a very important factor. In the work-related factors group, being in the contact with a SARS-CoV-2-positive colleague (OR = 2.35, p = 0.026) or being in contact with a COVID-19 patient (OR = 1.96, p = 0.004) correlated with seropositivity. In the primary work location/department group, the only significant association appeared among those working in surgical, ENT or ophthalmology departments. The results of the multivariate analysis further supported the thesis that the age of nurses and clinicians was the factor most strongly associated with seropositivity. The youngest nurses and clinicians were 12.5 times more likely to be seropositive than those in the oldest age group (p = 0.024). Being in contact with a SARS-CoV-2-positive family/household member remained the second most important factor. Conclusions A significant number of clinicians and nurses working in secondary healthcare were infected in the first 9 months of the pandemic.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Seroprevalence of Bordetella pertussis antibodies among a group of young adults
Autorzy:: Skiba, A.
Raman, S.
Rzucidlo, I.
Sereda, D.
Koziol, M.M.
Tematy:: seroprevalence
Bordetella pertussis
antibody
pertussis
respiratory system
adolescent
young adult; Pokaż więcej
Wydawca:: Instytut Medycyny Wsi
Powiązania:: https://bibliotekanauki.pl/articles/2098551.pdf Link otwiera się w nowym oknie
Opis:: Introduction. Bordetella pertussis is a Gram-negative rod which attacks the respiratory system and causes whooping cough disease. Despite the high percentage of childhood vaccination amongst the population an increasing number of pertussis cases have been observed over time, especially among adolescents and young adults. Objective. The aim of the study was to analyze the concentration of IgG anti-PT Bordetella antibodies in the serum of young adults in the aspect of maintaining immunity. Materials and method. The study group consisted of 30 patients, medical students aged 21–26 years. They were interviewed with a questionnaire, which asked about their general health, medications taken, basic information about whooping cough, or vaccination status against B. pertussis. The anti-PT Bordetella levels were measured in the collected serum by a commercial ELISA kit (enzyme-linked immunosorbent assay). Results. All patients had previously received a mandatory vaccination for pertussis as a child, and 36.6%had received a booster dose after the age of 18. The total average antibody level was 13.977 IU/mL (range: <5.00 – 50.74) and 40% of samples had a very low concentration (<5 IU/mL). Conclusions. Current findings suggest that post-vaccine immunity decreases over time, while infection susceptibility among younger age groups who have not yet been fully immunized has increased. Methods that could prevent and enable risk stratification of whooping cough include the screening test for anti-PT Bordetella antibody, the improvement of diagnostics, and an additional booster dose.
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Seroprevalence of anti-HAV total antibodies among workers in wastewater treatment plants
Autorzy:: Toseva, Elka I.
Atanasova, Mariya V.
Turnovska, Tanya H.
Tematy:: anti-HAV total antibodies
immunization
Hepatitis A
seroprevalence
wastewater
workers; Pokaż więcej
Wydawca:: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Powiązania:: https://bibliotekanauki.pl/articles/2159910.pdf Link otwiera się w nowym oknie
Opis:: Objectives Data on high frequency of hepatitis A virus (HAV) antibodies for wastewater treatment staff is contradictory. Literature lacks data on the seroprevalence of antibodies to HAV (anti-HAV) among workers in wastewater treatment plants (WWTPs) in Bulgaria. The aim of this study is to establish a specific humoral immune response to hepatitis A virus – anti-HAV total antibodies among staff in WWTPs. Material and Methods A complex study of health and working conditions included 110 subjects working in 3 WWTPs in Bulgaria (74% of all workers in the 3 studied WWTPs and 20% of all employees in Bulgaria registered in 2014 under the wastewater collection, discharge and treatment code of economic activity). Workers had been differentiated in 3 groups on the basis of their occupational work: operators, support staff and other workers exposed to biological agents. Venous blood from all 110 subjects was tested once for carriers of HAV antibodies. Results Anti-HAV total antibodies were found for 52.7% of workers in WWTPs. There is a positive association between activity performed in WWTPs (operators, maintenance personnel and others exposed) and a positive one for the presence of anti-HAV (Chi² = 6.882, df = 2, p = 0.032). Odds ratio (OR) for hepatitis A increases 2.9 times in the group of operators vs. others exposed to biological agents in WWTPs (OR = 2.914, 95% confidence interval (CI): 1.149–7.393, Fisher’s p = 0.039). Odds ratio for hepatitis A increases 4.3 times in the group of support staff from WWTPs vs. others exposed to biological agents in WWTP (OR = 4.295, 95% CI: 1.075–17.167, Fisher’s p = 0.049). Conclusions Higher frequency of anti-HAV antibodies among operators and maintenance personnel at WWTPs has been established as compared to other workers exposed to biological agents in WWTPs. There is a positive association between increasing age of the workers and the presence of anti-HAV. Int J Occup Med Environ Health 2018;31(3):307–315
Dostawca treści:: Biblioteka Nauki

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