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Przejdź do strony domowej biblioteki Pedagogiczna Biblioteka Wojewódzka im. Komisji Edukacji Narodowej w Lublinie Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaPedagogiczna Biblioteka Wojewódzka im. Komisji Edukacji Narodowej w Lublinie
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Wyświetlanie 1-10 z 33
Tytuł:
You will know by its tail: a method for quantification of heterogeneity of bacterial populations using single-cell MIC profiling
Autorzy:
Bogusławski, Jakub
Makuch, Karol
Garstecki, Piotr
Pacocha, Natalia
Zapotoczna, Marta
Wydawca:
Royal Society of Chemistry
Cytata wydawnicza:
Lab Chip, 2022, 22, 4317. https://doi.org/10.1039/D2LC00234E
Opis:
National Science Centre funding based on decision numbers DEC-2014/12/W/NZ6/00454 (Symphony) and Maestro 10 2018/30/A/ST4/00036. National Science Centre, Poland grant no. 2018/31/D/NZ6/02648 (SONATA14), the Foundation for Polish Science within the Team-Net POIR.04.04.00-0016ED/18-00 program, the Foundation for Polish Science within TEAM TECH POIR.04.04.00-00-2159/16-00, International Research Agendas program of the Foundation for Polish Science co-financed by the European Union under the European Regional Development Fund
Severe non-healing infections are often caused by multiple pathogens or by genetic variants of the same pathogen exhibiting different levels of antibiotic resistance. For example, polymicrobial diabetic foot infections double the risk of amputation compared to monomicrobial infections. Although these infections lead to increased morbidity and mortality, standard antimicrobial susceptibility methods are designed for homogenous samples and are impaired in quantifying heteroresistance. Here, we propose a droplet-based label-free method for quantifying the antibiotic response of the entire population at the single-cell level. We used Pseudomonas aeruginosa and Staphylococcus aureus samples to confirm that the shape of the profile informs about the coexistence of diverse bacterial subpopulations, their sizes, and antibiotic heteroresistance. These profiles could therefore indicate the outcome of antibiotic treatment in terms of the size of remaining subpopulations. Moreover, we studied phenotypic variants of a S. aureus strain to confirm that the profile can be used to identify tolerant subpopulations, such as small colony variants, associated with increased risks for the development of persisting infections. Therefore, the profile is a versatile instrument for quantifying the size of each bacterial subpopulation within a specimen as well as their individual and joined heteroresistance.
Dostawca treści:
Repozytorium Centrum Otwartej Nauki
Artykuł
Tytuł:
Applications of Arthrospira platensis as an Alternative Source of Food, Maintaining Nutritional Security and Awareness Creation; there by Reducing Problems of Malnutrition in the Society
Autorzy:
Degnechew, Genene Demisu
Buzayehu, Desisa Benti
Tematy:
Antioxidant
Arthrospira platensis
Cyanobacteria
Malnutrition
Single cell protein
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Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Powiązania:
https://bibliotekanauki.pl/articles/1111949.pdf  Link otwiera się w nowym oknie
Opis:
An increase in population has led to explorations of alternative sources of food, nutrition and energy. This search and the maintenance of nutritional security are essential dimensions of sustainable communal growth and development. This study revises evidences for alternative food production from Arthrospira platensis. The use of Arthrospira platensis for food is increasingly relevant as its components (proteins, lipids, minerals and vitamins) have substantial potential to be competitive with the same components from other food sources, and Arthrospira platensis-based food production is relatively low in price and holds medicinal properties.
Dostawca treści:
Biblioteka Nauki
Artykuł
Autorzy:
Małek, Kamilla
Wiercigroch, Ewelina
Majzner, Katarzyna
Dybaś, Jakub
Pacia, Marta
Barańska, Małgorzata
Zając, Grzegorz
Kaczor, Agnieszka
Jaworska, Aleksandra
Staniszewska-Ślęzak, Emilia
Opis:
Raman microscopy, a label-free method with high spatial resolution, shows growing potential in various fields of medical diagnostics. Several proof-of-concept studies related to the application of Raman microscopy to detect endothelial dysfunction are summarized in this work. Both ex vivo measurements of the tissues in the murine models of endothelial pathologies, as well as in vitro investigations of the cell cultures in the context of cellular transport, drug action and inflammation processes are discussed. The future directions in application of Raman spectroscopy-based methods in such studies are also described.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Autorzy:
Stanek, Ewa
Czamara, Krzysztof
Opis:
Perivascular adipose tissue (PVAT) has emerged as a dynamic organ influencing vascular function and cardiovascular health. In this brief review, an overview of the recent research in the investigation of PVAT is presented, ranging from in vivo studies to single-cell methodologies, in particular those based on Raman spectroscopy. The strengths and limitations of each, emphasizing their contributions to the current understanding of PVAT biology were discussed. Ultimately, the integration of these diverse methodologies promises to uncover new therapeutic targets and diagnostic biomarkers, including those emerging from simple Raman spectroscopy-based measurements of alterations in lipid unsaturation degree, invariably associated with PVAT dysfunction.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Artykuł
Tytuł:
Comparison of the effects of bleomycin and ionizing radiation in two sublines of murine lymphoma L5178Y
Autorzy:
Kruszewski, M.
Zaim, J.
Grądzka, I.
Szumiel, I.
Tematy:
bleomycin
comet assay
DNA damage
gamma radiation
single cell gel electrophoresis
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Wydawca:
Instytut Chemii i Techniki Jądrowej
Powiązania:
https://bibliotekanauki.pl/articles/147561.pdf  Link otwiera się w nowym oknie
Opis:
We compared the effects of bleomycin (BLM) and ionizing radiation on two sublines of murine lymphoma L5178Y (LY): LY-R, radiation resistant and LY-S, radiation sensitive. This radiosensitivity difference is related to the ability to rejoin DNA double strand breaks. LY-S cells were about two times more sensitive to BLM than LY-R, similarly as in the case of sensitivity to X rays. Since there was no difference in the P-glycoprotein-related drug transport system between the sublines, it could be expected that the enhanced sensitivity of LY-S cells to BLM was caused by the DNA repair defect. Growth disturbances in BLM treated cell populations were proportional to the lethal effect and their duration was observed until elimination of dead cells (3-6 days after 50 ěM BLM, 1 h at 37oC). There was no slow growth phase accompanied by normal viability, as previously described for X-irradiated LY-S cells. Initial DNA damage, estimated with the single cell gel electrophoresis method was linearly related to BLM dose in LY-S cells; in LY-R cells - in the low dose range (up to 10 ěM) - there was more damage than in LY-S cells, however, at higher doses the dose - effect curves became identical. The doseeffect relationship for ă rays was linear and identical in both cell sublines. DNA damage distribution in BLM treated cells was much less uniform as compared to that in irradiated cells and indicated the presence of cells with severely damaged DNA, a feature typical for BLM action in vitro.
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 33

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