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Wyświetlanie 1-10 z 93
Tytuł:
Evaluation of the suitability of mitochondrial DNA for species identification of microtraces and forensic traces
Autorzy:
Natonek-Wiśniewska, Małgorzata
Krzyścin, Piotr
Tematy:
biological traces
forensic DNA analysis
species identification of forensic DNA
species identification of biological traces
mtDNA
Pokaż więcej
Wydawca:
Polskie Towarzystwo Biochemiczne
Powiązania:
https://bibliotekanauki.pl/articles/1038564.pdf  Link otwiera się w nowym oknie
Opis:
The objective of the study was to demonstrate how mitochondrial DNA (mtDNA) can be used to determine the species origin of animal microtraces. The study included pieces of cat and dog hair without the root, a fragment of cooked chicken bone (0.1g), three goose down samples (0.028 g), a pork swab, a pork scratching (5×5×5 mm), and pork lard (0.22 g). DNA was isolated from all of these samples using the method appropriate for the particular source material. The extracts had DNA concentration exceeding 5.4 ng/µl with A260/280 purity range of 1.14-1.88. Next, the samples were subjected to PCR and real-time PCR with species-specific primers and primers complementary to mitochondrial DNA (mtDNA). Control reactions based on the amplification of eukaryotic-specific fragment (18S rRNA) were additionally performed. PCR and real-time PCR products for detection of species-specific mtDNA were obtained for all templates, whereas during the detection of eukaryote DNA no product was obtained for dog and cat hair only. The poor quality of the obtained DNA did not prevent the analysis. The results showed that mitochondrial DNA is suitable for identification of small or highly processed samples, in which genomic DNA often cannot be analyzed.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of tin species released in solution during water leaching of solids from the alkaline smelting (NaOH-KOH) of stanniferous ores
Autorzy:
Yuma, Phalaris M.
Kitungwa, Bertin K.
Mubiayi, Pierre Kalenga
Shengo, Michel L.
Moloto, Nosipho
Ngubeni, Grace
Mbayo, Marsi K.
Kyona, Crépin W.
Tematy:
tin ore
alkaline smelting
solids leaching
characterisation
tin species identification
Pokaż więcej
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Powiązania:
https://bibliotekanauki.pl/articles/58971094.pdf  Link otwiera się w nowym oknie
Opis:
Tin species were studied from Makundju ores in DRCongo. The identification of tin species released in aqueous solution during leaching of solids from the alkaline smelting (NaOH-KOH) was done to prepare added-value mining products on tin ores mined in the eastern DR Congo. Prior to alkaline fusion to have the smelting products (SP), the initial sample (IS) was processed for tests and characterization of tin species. Alkaline fusion in molten KOH-NaOH system was used and leached solution was considered for further analyses. Various physicochemical techniques including X-ray fluorescence spectrometry (XRF), scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and FTIR spectroscopy were used to characterize the initial ore and the molten cake. Water leached cake solution at a solid/liquid ratio of 1:10 was used to identify and characterize tin species. ICP-OES analysis of the pH 11.9 solution revealed a content of 4506.3 ppm, which is equivalent to a 95.87% leaching yield. Such yield attests to the stable Sn(OH)2-6(aq) ion as confirmed by UV-Vis. Crystallizations of M2Sn(OH) 6(s) (M = Na and/or K) was studied using XRD, FTIR and TGA. As pH decreases below 9, Sn(OH) )2-6(aq) ion changes to other tin species such as Sn(OH)-5(aq), Sn(OH)4(S), Sn(OH)+3(aq), Sn(OH)2+2(aq), Sn(OH)3+(aq) and Sn4+(aq). The stability of each the tinspecies was determined at different pH values in aqueous solution. Hence, alkaline hexahydroxostannate nanoparticles, used in many electronic applications, can be produced from tin ores.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kod kreskowy DNA – praktyczne narzędzie taksonomii i inwentaryzacji entomofauny
DNA barcoding: A practical tool for taxonomy and species identification of entomofauna
Autorzy:
Szyp-Borowska, I.
Sikora, K.
Tematy:
entomologia
owady
taksonomia
identyfikacja gatunkowa
DNA barcoding
DNA barcodes
entomofauna
species identification
taxonomy
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Wydawca:
Instytut Badawczy Leśnictwa
Powiązania:
https://bibliotekanauki.pl/articles/1291234.pdf  Link otwiera się w nowym oknie
Opis:
DNA barcoding is an innovative system designed to provide rapid, accurate, and automatable species identification by using short, standardized gene regions as internal species codes. The mitochondrial cytochrome C oxidase I (COI) gene was proposed by Paul Hebert as an official marker for animals, because of its small intraspecific but large interspecific variation. Since the launch of the project Barcode of Life, this simple technique has caught the interest of taxonomists, ecologists and plantquarantine officers charged with the control of pests and invasive species. The great diversity of insects and their importance have made this group a major target for DNA barcoding. In most cases, the identification of insect species by traditional methods based on morphological features requires specialist knowledge and is labor-intensive. DNA barcoding aims at meeting the challenge of monitoring and documenting the biodiversity of insects. The utility of DNA barcoding for identifying small insects, cryptic taxa or rare species, as well as many species of forest entomofauna that are impossible to discriminate morphologically throughout all of their life stages, is a subject discussed in this review. Due to its usefulness, also in Poland in the Forestry Research Institute, a method for identifying selected species of saproxylic beetles based on the sequence of the COI region was developed. In the future, this method will be used to assess the state of biodiversity and the naturalness of forest ecosystems. Therefore, this and other future implications of this promising new technique are also discussed here.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kod kreskowy DNA – praktyczne narzędzie taksonomii i inwentaryzacji entomofauny
DNA barcoding: A practical tool for taxonomy and species identification of entomofauna
Autorzy:
Szyp-Borowska, Iwona
Sikora, Katarzyna
Wydawca:
Instytut Badawczy Leśnictwa (Forest Research Institute), Sękocin Stary, Poland
Cytata wydawnicza:
Szyp-Borowska, I., & Sikora, K. (2019). DNA barcoding: A practical tool for the taxonomy and species identification of entomofauna, Forest Research Papers, 80(3), 227-232. doi: https://doi.org/10.2478/frp-2019-0021.
Opis:
DNA barcoding is an innovative system designed to provide rapid, accurate, and automatable species identification by using short, standardized gene regions as internal species codes. The mitochondrial cytochrome C oxidase I (COI) gene was proposed by Paul Hebert as an official marker for animals, because of its small intraspecific but large interspecific variation. Since the launch of the project Barcode of Life, this simple technique has caught the interest of taxonomists, ecologists and plant-quarantine officers charged with the control of pests and invasive species. The great diversity of insects and their importance have made this group a major target for DNA barcoding. In most cases, the identification of insect species by traditional methods based on morphological features requires specialist knowledge and is labor-intensive. DNA barcoding aims at meeting the challenge of monitoring and documenting the biodiversity of insects. The utility of DNA barcoding for identifying small insects, cryptic taxa or rare species, as well as many species of forest entomofauna that are impossible to discriminate morphologically throughout all of their life stages, is a subject discussed in this review. Due to its usefulness, also in Poland in the Forestry Research Institute, a method for identifying selected species of saproxylic beetles based on the sequence of the COI region was developed. In the future, this method will be used to assess the state of biodiversity and the naturalness of forest ecosystems. Therefore, this and other future implications of this promising new technique are also discussed here.
Dostawca treści:
Repozytorium Centrum Otwartej Nauki
Artykuł
Tytuł:
Production of pure tantalum and niobium oxides by hydrometallurgical processing of coltan smelter cake from Tanganyika Province/RD Congo using NH4HF2-KOH flux system
Autorzy:
Kitungwa, Bertin K.
Yuma, Phalaris M.
Kalenga, Mubiayi. Pierre
Shengo, Michel L.
Ngubeni, Grace
Moloto, Nosipho
Mbayo, Marsi K.
Kyona, Crépin W.
Tematy:
Molten Coltan ore
ammonium bifluoride
potassium hydroxide
hydrometallurgical recovery of Ta and Nb
species identification
Pokaż więcej
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Powiązania:
https://bibliotekanauki.pl/articles/58970994.pdf  Link otwiera się w nowym oknie
Opis:
Pure tantalum and niobium oxides were produced from the molten cake after mixing oreNH4HF2-KOH in a respective mass ratio of 1/4/3. This resulted in solubilization yields of over 95% for Nb and around 92% for Ta in sulfuric acid solution 3 molar. Extraction of tantalum and niobium using octan-2-ol yielded over 95% niobium and 98% tantalum when pH is set at 0.5-1.0 and 1.5-2 respectively. The compounds K3NbOF6, K3NbO4 and K2TaF7 were identified after melting. While in aqueous solution ionic species such as NbF6 (OH) 23-, NbF6- and TaF72- are likely to be identified. Precipitation of tantalum and niobium in NH4OH solution (pH=7.50-8.0) as hydrated oxides after stripping with distilled water and crystallization identified hydrated oxides such as Ta2O5.nH2O, Nb2O5.nH2O. These two oxides were associated with a small amount of SiO2.nH2O as an impurity resulting from the extraction and precipitation of tantalum and niobium. SEM-EDS, XRD, TGA-DTG and FTIR analyses identified and characterized these compounds.
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identyfikacja gatunków na podstawie sekwencji DNA
Species identification based on DNA sequences
Autorzy:
Brol, Hubert
Opis:
Protura to stosunkowo późno odkryty takson kryptycznych Hexapoda o kontrowersyjnej pozycji systematycznej. Morfologiczna identyfikacja Protura jest niezwykle trudna ze względu na znaczną liczbę gatunków (ponad 800) i bardzo niewielkie różnice morfologiczne przy jednoczesnych małych rozmiarach. Aby przeanalizować możliwość ich identyfikacji do gatunku, wykorzystano barkoding, bazując na sekwencji podjednostki rybosomalnej 28SrRNA, a następnie porównanie z danymi morfologicznymi. Zastosowano również ilościową metodę delimitacji gatunków ABGD. Z uwagi na znaczną trudność z morfologiczną identyfikacją Protura nie da się jednoznacznie zinterpretować wyników analiz molekularnych. Wydaje się jednak, że wykorzystana długość badanego fragmentu 28SrRNA jest zbyt mała, aby można było na jej podstawie właściwie zastosować analizę ABGD.
Protura are a relatively lately discovered taxon of cryptic Hexapoda, known for their controversial systematic position. Morphological identification of Protura is extraordinarily hard given the significant number of species (over 800), very minor morphological differences and their small size. Barcoding based on 28SrRNA ribosomal subunit sequence was employed in order to analyze the possibility of identifying proturans to species level. Obtained information was then compared with morphological data. ABGD analysis was used as a means of quantitative species delimitation. Due to a significant trouble with morphological identification of Protura, it is impossible to interpret molecular data unambiguously. It is, however, likely that the length of the used fragment of 28SrRNA is too short to properly conduct ABGD analysis.
Dostawca treści:
Repozytorium Uniwersytetu Jagiellońskiego
Inne
Wyświetlanie 1-10 z 93

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