- Tytuł:
- The role of benzoate secreted by Desulfotomaculum acetoxidans DSM 771 in sulfate uptake
- Autorzy:
-
Pawłowska-Ćwięk, Lucyna
Pado, Ryszard - Tematy:
-
sulfonation
benzoate secretion
oxidation - Pokaż więcej
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Powiązania:
- https://bibliotekanauki.pl/articles/1041319.pdf Link otwiera się w nowym oknie
- Opis:
- This work was designed to find the cause of the delay in hydrogen sulfide dissimilation in Desulfotomaculum acetoxidans DSM 771, which is dependent on the sulfate uptake. This bacterium grown without addition of any aromatic compound was shown by spectrum analysis with the methylene method to contain hydroxy-benzoate derivatives. The presence of these compounds was confirmed by HPLC in fractions obtained from cell walls after 15 days of culture. The test with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt seemed to indicate the presence of peroxidase, which probably oxidized benzoate to its hydroxy derivatives. The test with 5-sulfo-salicylic acid proved the ability of the investigated strain to utilize arylsulfates and to reduce sulfate group to hydrogen sulfide. On the basis of the above data, we propose the following sequence of reactions: 1, benzoate secretion; 2, benzoate hydroxylation; 3, sulfonation of hydroxy-benzoate derivatives.
- Dostawca treści:
- Biblioteka Nauki
Artykuł